Rabbit Anti-KRT6A Monoclonal Antibody – Cat# MBS1750157

Western blot analysis of Cytokeratin 6 expression in A431 cell lysate (MBS1750157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT6A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT6A

Cytokeratin 6 (KRT6A) Antibody – Rabbit Monoclonal

Name: KRT6A Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750157
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750157

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 6 (KRT6A), Monoclonal Antibody

Full Product Name

Anti-Cytokeratin 6 Rabbit Monoclonal Antibody

Product Gene Name

anti-KRT6A antibody

Product Synonym Names

Keratin; type II cytoskeletal 6A; Cytokeratin-6A; CK-6A; Cytokeratin-6D; CK-6D; Keratin-6A; K6A; Type-II keratin Kb6; Hom s 5; KRT6A; K6A; KRT6D

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

60045 MW

3D Structure

ModBase 3D Structure for P02538

Immunogen

A synthesized peptide derived from human Cytokeratin 6

Tissue Specificity

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-KRT6A antibody

Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; cytokeratin; Signal Transduction; Structures; Cytoskeleton; Intermediate Filaments; class i; microtubules

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-KRT6A antibody

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-KRT6A antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-KRT6A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT6A purchase

MBS1750157 is an antibody targeting KRT6A. Also known as Anti-Cytokeratin 6 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT6A. It may not necessarily be applicable to this product.

NCBI GI #

5031839

NCBI GeneID

3853

NCBI Accession #

NP_005545.1

UniProt Primary Accession #

P02538

UniProt Secondary Accession #

P48667; Q08AR4; Q6NT67; Q96CL4; A4QPC1

UniProt Related Accession #

P48668

GenBank Nucleotide #

NM_005554.3

NCBI Official Full Name

keratin, type II cytoskeletal 6C

NCBI Official Synonym Full Names

keratin 6A

NCBI Official Symbol

KRT6A

NCBI Official Synonym Symbols

K6A; K6C; K6D; PC3; CK6A; CK6C; CK6D; CK-6C; CK-6E; KRT6C; KRT6D

UniProt Protein Name

Keratin, type II cytoskeletal 6A

UniProt Synonym Protein Names

Cytokeratin-6A; CK-6A; Cytokeratin-6D; CK-6D; Keratin-6A; K6A; Type-II keratin Kb6; Allergen: Hom s 5

Protein Family

Keratin

UniProt Gene Name

KRT6A

UniProt Synonym Gene Names

K6A; KRT6D; CK-6A; CK-6D; K6A

NCBI Protein Information

keratin, type II cytoskeletal 6C

NCBI Summary for KRT6A

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]

Research Articles on KRT6A

1. KRT6A genetic mutation is associate with the development of Pachyonychia Congenita in patients in Australia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT6A antibody

Reference Product	PubMed Publications
KRT6B antibody	>33 publications with KRT6A and KRT6B
KRT17 antibody	>21 publications with KRT6A and KRT17

Diseases associated with anti-KRT6A antibody

Disease Name	Pubmed Publications
Pachyonychia Congenita Antibodies	>36 publications with KRT6A and Pachyonychia Congenita
Carcinoma Antibodies	>12 publications with KRT6A and Carcinoma
Breast Neoplasms Antibodies	>8 publications with KRT6A and Breast Neoplasms
Lung Neoplasms Antibodies	>5 publications with KRT6A and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>4 publications with KRT6A and Carcinoma, Squamous Cell
Neoplasm Invasiveness Antibodies	>4 publications with KRT6A and Neoplasm Invasiveness
Lung Diseases Antibodies	>4 publications with KRT6A and Lung Diseases
Neoplasm Metastasis Antibodies	>3 publications with KRT6A and Neoplasm Metastasis
Inflammation Antibodies	>2 publications with KRT6A and Inflammation
Precancerous Conditions Antibodies	>2 publications with KRT6A and Precancerous Conditions

Organs/Tissues associated with anti-KRT6A antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>23 publications with KRT6A and Skin
Lung Antibodies	>5 publications with KRT6A and Lung
Muscle Antibodies	>2 publications with KRT6A and Muscle
Kidney Antibodies	>2 publications with KRT6A and Kidney
Mouth Antibodies	>2 publications with KRT6A and Mouth
Lymph Node Antibodies	>2 publications with KRT6A and Lymph Node
Vascular Antibodies	>1 publications with KRT6A and Vascular
Mammary Gland Antibodies	>1 publications with KRT6A and Mammary Gland
Thyroid Antibodies	>1 publications with KRT6A and Thyroid
Prostate Antibodies	>1 publications with KRT6A and Prostate