Rabbit Anti-CDC6 Monoclonal Antibody – Cat# MBS1750256

Western blot analysis of Phospho-Cdc6 (S54) expression in (1) Raji cell lysate; (2) Raji + FBS cell lysate (MBS1750256).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC6

CDC6 Antibody – Rabbit Monoclonal

Name: CDC6 Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750256
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750256

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cdc6, Monoclonal Antibody

Full Product Name

Anti-Phospho-Cdc6 (S54) Rabbit Monoclonal Antibody

Product Gene Name

anti-Cdc6 antibody

Product Synonym Names

Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6; CDC6; CDC18L

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

62720 MW

OMIM

U77949 mRNA

3D Structure

ModBase 3D Structure for Q99741

Immunogen

A synthesized peptide derived from human Phospho-Cdc6 (S54)

Subcellular Localization

Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-Cdc6 antibody

Epigenetics and Nuclear Signaling; DNA/RNA; dna Synthesis; Cell Biology; Cell Cycle; Cell Division; Other cell division Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Cdc6 antibody

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-Cdc6 antibody

WB: 1:500-1:1000
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-Cdc6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdc6 purchase

MBS1750256 is an antibody targeting Cdc6. Also known as Anti-Phospho-Cdc6 (S54) Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cdc6. It may not necessarily be applicable to this product.

NCBI GI #

4502703

NCBI GeneID

990

NCBI Accession #

NP_001245.1

UniProt Primary Accession #

Q99741

UniProt Secondary Accession #

Q8TB30

UniProt Related Accession #

Q99741

GenBank Nucleotide #

NM_001254.3

NCBI Official Full Name

cell division control protein 6 homolog

NCBI Official Synonym Full Names

cell division cycle 6

NCBI Official Symbol

CDC6

NCBI Official Synonym Symbols

CDC18L; HsCDC6; MGORS5; HsCDC18

UniProt Protein Name

Cell division control protein 6 homolog

UniProt Synonym Protein Names

CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6

Protein Family

Cell division control protein

UniProt Gene Name

CDC6

UniProt Synonym Gene Names

CDC18L; HsCdc18; HsCDC6

NCBI Protein Information

cell division control protein 6 homolog

NCBI Summary for Cdc6

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Research Articles on Cdc6

1. WHSC1L1 and H3K36me2 are enriched in the gene bodies of the cell cycle-related genes CDC6 and CDK2, implying that WHSC1L1 directly regulates the transcription of these gene

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cdc6 antibody

Reference Product	PubMed Publications
MCM2 antibody	>106 publications with Cdc6 and MCM2
CDT1 antibody	>105 publications with Cdc6 and CDT1
ORC1 antibody	>84 publications with Cdc6 and ORC1
CDK2 antibody	>70 publications with Cdc6 and CDK2
PCNA antibody	>31 publications with Cdc6 and PCNA
MCM7 antibody	>31 publications with Cdc6 and MCM7
ORC2 antibody	>30 publications with Cdc6 and ORC2
ORC6 antibody	>23 publications with Cdc6 and ORC6
MCM3 antibody	>23 publications with Cdc6 and MCM3
E2F1 antibody	>18 publications with Cdc6 and E2F1

Pathways associated with anti-Cdc6 antibody

Products by Pathway	Pathway Diagram
Activation Of ATR In Response To Replication Stress Pathway antibodies	Activation Of ATR In Response To Replication Stress Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Assembly Of The Pre-replicative Complex Pathway antibodies	Assembly Of The Pre-replicative Complex Pathway Diagram
Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies	Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
CDC6 Association With The ORC:origin Complex Pathway antibodies	CDC6 Association With The ORC:origin Complex Pathway Diagram
CDK-mediated Phosphorylation And Removal Of Cdc6 Pathway antibodies	CDK-mediated Phosphorylation And Removal Of Cdc6 Pathway Diagram
CDT1 Association With The CDC6:ORC:origin Complex Pathway antibodies	CDT1 Association With The CDC6:ORC:origin Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram

Diseases associated with anti-Cdc6 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>86 publications with Cdc6 and Neoplasms
Cell Transformation, Neoplastic Antibodies	>12 publications with Cdc6 and Cell Transformation, Neoplastic
Adenocarcinoma Antibodies	>12 publications with Cdc6 and Adenocarcinoma
Breast Neoplasms Antibodies	>11 publications with Cdc6 and Breast Neoplasms
Prostatic Neoplasms Antibodies	>10 publications with Cdc6 and Prostatic Neoplasms
Carcinoma, Hepatocellular Antibodies	>8 publications with Cdc6 and Carcinoma, Hepatocellular
Lung Neoplasms Antibodies	>7 publications with Cdc6 and Lung Neoplasms
Liver Diseases Antibodies	>6 publications with Cdc6 and Liver Diseases
Neoplasm Metastasis Antibodies	>6 publications with Cdc6 and Neoplasm Metastasis
Meier-Gorlin syndrome Antibodies	>5 publications with Cdc6 and Meier-Gorlin syndrome

Organs/Tissues associated with anti-Cdc6 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>16 publications with Cdc6 and Blood
Prostate Antibodies	>16 publications with Cdc6 and Prostate
Lung Antibodies	>13 publications with Cdc6 and Lung
Bone Antibodies	>10 publications with Cdc6 and Bone
Muscle Antibodies	>9 publications with Cdc6 and Muscle
Liver Antibodies	>8 publications with Cdc6 and Liver
Lymph Node Antibodies	>8 publications with Cdc6 and Lymph Node
Lymph Antibodies	>8 publications with Cdc6 and Lymph
Vascular Antibodies	>6 publications with Cdc6 and Vascular
Cervix Antibodies	>6 publications with Cdc6 and Cervix