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SMC1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750272

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous… More details

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Product Overview

Product Name
SMC1, Monoclonal Antibody
Full Product Name
Anti-Phospho-SMC1 (S957) Rabbit Monoclonal Antibody
Product Gene Name
anti-SMC1 antibody
Product Synonym Gene Name
SMC1A
Product Synonym Names
Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8; SMC1A; DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
143233 MW
OMIM
S78271 mRNA
3D Structure
ModBase 3D Structure for Q14683
Immunogen
A synthesized peptide derived from human Phospho-SMC1 (S957)
Subcellular Localization
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SMC1 antibody

Cell Biology; Cell Cycle; Cell Division; chromatid cohesion
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SMC1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-SMC1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-SMC1 antibody

Western blot analysis of Phospho-SMC1 (S957) expression in (1) HeLa cell lysate; (2) Hela cells lysate treated with AP (MBS1750272).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMC1A
Rabbit Anti-SMC1 Monoclonal Antibody – Cat# MBS1750272

Other Notes

Small volumes of anti-SMC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMC1 purchase

MBS1750272 is an antibody targeting SMC1. Also known as Anti-Phospho-SMC1 (S957) Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMC1. It may not necessarily be applicable to this product.

NCBI GI #
30581135
NCBI GeneID
8243
NCBI Accession #
NP_006297.2
UniProt Primary Accession #
Q14683
UniProt Secondary Accession #
O14995; Q16351; Q2M228
UniProt Related Accession #
Q14683
GenBank Nucleotide #
NM_006306.3
NCBI Official Full Name
structural maintenance of chromosomes protein 1A isoform 1
NCBI Official Synonym Full Names
structural maintenance of chromosomes 1A
NCBI Official Symbol
SMC1A
NCBI Official Synonym Symbols
SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
UniProt Protein Name
Structural maintenance of chromosomes protein 1A
UniProt Synonym Protein Names
Sb1.8
Protein Family
Structural maintenance of chromosomes protein
UniProt Gene Name
SMC1A
UniProt Synonym Gene Names
DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
NCBI Protein Information
structural maintenance of chromosomes protein 1A

NCBI Summary for SMC1

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
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Research Articles on SMC1

1. We conclude that SMC1A variants can result in a phenotype resembling CdLS and a phenotype resembling Rett syndrome. Resemblances between the SMC1A group and the NIPBL group suggest that a disturbed cohesin function contributes to the phenotype, but differences between these groups may also be explained by other underlying mechanisms such as moonlighting of the cohesin genes.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMC1 antibody

 Reference Product  PubMed Publications
 SMC3 antibody  >28 publications with SMC1 and SMC3
 RAD21 antibody  >9 publications with SMC1 and RAD21
 ATM antibody  >1 publications with SMC1 and ATM

Pathways associated with anti-SMC1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram

Diseases associated with anti-SMC1 antibody

 Disease Name  Pubmed Publications
 CdLS, X-Linked Antibodies  >37 publications with SMC1 and CdLS, X-Linked
 Neoplasms Antibodies  >5 publications with SMC1 and Neoplasms
 Heart Diseases Antibodies  >4 publications with SMC1 and Heart Diseases
 Death Antibodies  >4 publications with SMC1 and Death
 Cardiomyopathies Antibodies  >1 publications with SMC1 and Cardiomyopathies
 Precancerous Conditions Antibodies  >1 publications with SMC1 and Precancerous Conditions
 Immune System Diseases Antibodies  >1 publications with SMC1 and Immune System Diseases
 Hypertrophy Antibodies  >1 publications with SMC1 and Hypertrophy

Organs/Tissues associated with anti-SMC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >4 publications with SMC1 and Bone
 Heart Antibodies  >4 publications with SMC1 and Heart
 Blood Antibodies  >3 publications with SMC1 and Blood
 Muscle Antibodies  >2 publications with SMC1 and Muscle
 Mouth Antibodies  >2 publications with SMC1 and Mouth
 Lung Antibodies  >2 publications with SMC1 and Lung
 Bone Marrow Antibodies  >2 publications with SMC1 and Bone Marrow
 Eye Antibodies  >2 publications with SMC1 and Eye
 Esophagus Antibodies  >1 publications with SMC1 and Esophagus
 Embryonic Tissue Antibodies  >1 publications with SMC1 and Embryonic Tissue
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