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HSL Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750287

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSL, Monoclonal Antibody
Full Product Name
Anti-Phospho-HSL (S853) Rabbit Monoclonal Antibody
Product Gene Name
anti-HSL antibody
Product Synonym Gene Name
LIPE
Product Synonym Names
Hormone-sensitive lipase; HSL; 3.1.1.79; LIPE
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
116598 MW
OMIM
L11706 Genomic DNA
3D Structure
ModBase 3D Structure for Q05469
Immunogen
A synthesized peptide derived from human Phospho-HSL (S853)
Subcellular Localization
Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HSL antibody
Western Blot (WB)
Application Notes for anti-HSL antibody
WB: 1:5000-1:10000

Western Blot (WB) of anti-HSL antibody

Western blot analysis of Phospho-HSL (S853) expression in (1) Mouse muscle lysate; (2) Mouse muscle lysate treated with AP (MBS1750287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIPE monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LIPE
Rabbit Anti-HSL Monoclonal Antibody – Cat# MBS1750287

Other Notes

Small volumes of anti-HSL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSL purchase

MBS1750287 is an antibody targeting HSL. Also known as Anti-Phospho-HSL (S853) Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSL. It may not necessarily be applicable to this product.

NCBI GI #
21328446
NCBI GeneID
3991
NCBI Accession #
NP_005348.2
UniProt Primary Accession #
Q05469
UniProt Secondary Accession #
Q3LRT2; Q6NSL7
UniProt Related Accession #
Q05469
GenBank Nucleotide #
NM_005357.3
NCBI Official Full Name
hormone-sensitive lipase
NCBI Official Synonym Full Names
lipase E, hormone sensitive type
NCBI Official Symbol
LIPE
NCBI Official Synonym Symbols
HSL; LHS; AOMS4; FPLD6
UniProt Protein Name
Hormone-sensitive lipase
Protein Family
HTH-type quorum sensing-dependent transcriptional regulator
UniProt Gene Name
LIPE
UniProt Synonym Gene Names
HSL
NCBI Protein Information
hormone-sensitive lipase

NCBI Summary for HSL

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
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Research Articles on HSL

1. The homozygous null LIPE mutation could result in marked inhibition of lipolysis from some adipose tissue depots and thus may induce an extremely rare phenotype of MSL and partial lipodystrophy in adulthood associated with complications of insulin resistance, such as diabetes, hypertriglyceridemia and hepatic steatosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HSL antibody

 Reference Product  PubMed Publications
 FABP4 antibody  >2 publications with HSL and FABP4
 PRKACA antibody  >1 publications with HSL and PRKACA

Pathways associated with anti-HSL antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies  Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-HSL antibody

 Disease Name  Pubmed Publications
 Obesity Antibodies  >19 publications with HSL and Obesity
 Insulin Resistance Antibodies  >11 publications with HSL and Insulin Resistance
 Cardiovascular Diseases Antibodies  >11 publications with HSL and Cardiovascular Diseases
 Drug Toxicity Antibodies  >8 publications with HSL and Drug Toxicity
 Atherosclerosis Antibodies  >6 publications with HSL and Atherosclerosis
 Muscular Diseases Antibodies  >6 publications with HSL and Muscular Diseases
 Hypertension Antibodies  >4 publications with HSL and Hypertension
 Weight Gain Antibodies  >4 publications with HSL and Weight Gain
 Fatty Liver Antibodies  >3 publications with HSL and Fatty Liver
 Diabetes Mellitus Antibodies  >3 publications with HSL and Diabetes Mellitus

Organs/Tissues associated with anti-HSL antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with HSL and Blood
 Brain Antibodies  >29 publications with HSL and Brain
 Adipose Tissue Antibodies  >19 publications with HSL and Adipose Tissue
 Kidney Antibodies  >6 publications with HSL and Kidney
 Lung Antibodies  >5 publications with HSL and Lung
 Placenta Antibodies  >4 publications with HSL and Placenta
 Embryonic Tissue Antibodies  >4 publications with HSL and Embryonic Tissue
 Eye Antibodies  >3 publications with HSL and Eye
 Adrenal Gland Antibodies  >3 publications with HSL and Adrenal Gland
 Bone Antibodies  >3 publications with HSL and Bone
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