Rabbit Anti-IL10 Polyclonal Antibody – Cat# MBS1750306

Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (MBS1750306).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.

IL10 Antibody – Rabbit Polyclonal

Name: IL10 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750306
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750306

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL10, Polyclonal Antibody

Full Product Name

Anti-IL10 Picoband Antibody

Product Gene Name

anti-IL10 antibody

Product Synonym Names

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

20,641 Da

3D Structure

ModBase 3D Structure for P18893

Immunogen

E Coli-derived mouse IL10 recombinant protein (Position: S19-S178).

Subcellular Localization

Secreted.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-IL10 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-IL10 antibody

WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml

Other Notes

Small volumes of anti-IL10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL10 purchase

MBS1750306 is an antibody targeting IL10. Also known as Anti-IL10 Picoband Antibody. Reactive in Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL10. It may not necessarily be applicable to this product.

NCBI GI #

6754318

NCBI GeneID

16153

NCBI Accession #

NP_034678.1

UniProt Primary Accession #

P18893

UniProt Secondary Accession #

Q0VBJ1

UniProt Related Accession #

P18893

GenBank Nucleotide #

NM_010548.2

NCBI Official Full Name

interleukin-10

NCBI Official Synonym Full Names

interleukin 10

NCBI Official Symbol

Il10

NCBI Official Synonym Symbols

CSIF; Il-10

UniProt Protein Name

Interleukin-10

UniProt Synonym Protein Names

Cytokine synthesis inhibitory factor; CSIF

Protein Family

Interleukin

UniProt Gene Name

Il10

UniProt Synonym Gene Names

Il-10; IL-10; CSIF

NCBI Protein Information

interleukin-10

NCBI Summary for IL10

This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]

Research Articles on IL10

1. Mechanistic studies suggest a PKC-Syk-mediated signaling pathway, to which IL-10 conversely inhibits, is required for activating macrophage self-targeting, followed by phagocytosis independent of calreticulin Moreover, we identified spleen red pulp to be one specific tissue that provides stimuli constantly activating macrophage phagocytosis albeit lacking in Cd47(-/-) or Sirpalpha(-/-) mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL10 antibody

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram

Diseases associated with anti-IL10 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>8111 publications with IL10 and Necrosis
Inflammation Antibodies	>6630 publications with IL10 and Inflammation
Sepsis Antibodies	>1199 publications with IL10 and Sepsis
Colitis Antibodies	>944 publications with IL10 and Colitis
Genetic Predisposition to Disease Antibodies	>929 publications with IL10 and Genetic Predisposition to Disease
Hypersensitivity, Immediate Antibodies	>839 publications with IL10 and Hypersensitivity, Immediate
Inflammatory Bowel Diseases Antibodies	>791 publications with IL10 and Inflammatory Bowel Diseases
Arthritis, Rheumatoid Antibodies	>592 publications with IL10 and Arthritis, Rheumatoid
Kidney Diseases Antibodies	>490 publications with IL10 and Kidney Diseases
HIV Infections Antibodies	>433 publications with IL10 and HIV Infections

Organs/Tissues associated with anti-IL10 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>16353 publications with IL10 and Blood
Bone Marrow Antibodies	>1079 publications with IL10 and Bone Marrow