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ICOS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750366

Description: Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important… More details

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Product Overview

Online Datasheet in PDF
Product Name
ICOS, Polyclonal Antibody
Full Product Name
Anti-ICOS Picoband antibody
Product Gene Name
anti-ICOS antibody
Product Synonym Names
Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
19,127 Da
OMIM
AJ277832 mRNA
3D Structure
ModBase 3D Structure for Q9Y6W8
Immunogen
E Coli-derived human ICOS recombinant protein (Position: E21-K140).
Subcellular Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ICOS antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-ICOS antibody
Immunohistochemistry (Paraffin-embedded Section): 0.5-1mug/ml
Immunohistochemistry(Frozen Section): 0.5-1mug/ml
ICC: 0.5-1mug/ml
FC/FACS: 1-3g/1x10 6 cells
Direct ELISA: 0.1-0.5mug/ml

Immunohistochemistry (IHC) of anti-ICOS antibody

Figure 1. IHC analysis of ICOS using anti-ICOS antibody (MBS1750366).
ICOS was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ICOS Antibody (MBS1750366) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-ICOS Polyclonal Antibody – Cat# MBS1750366

Flow Cytometry (FC/FACS) of anti-ICOS antibody

Figure 2. Flow Cytometry analysis of Raji cells using anti-ICOS antibody (MBS1750366).
Overlay histogram showing Raji cells stained with MBS1750366 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICOS Antibody (MBS1750366,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-ICOS Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1750366

Other Notes

Small volumes of anti-ICOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICOS purchase

MBS1750366 is an antibody targeting ICOS. Also known as Anti-ICOS Picoband antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ICOS. It may not necessarily be applicable to this product.

NCBI GI #
15029518
NCBI GeneID
29851
NCBI Accession #
NP_036224.1
UniProt Primary Accession #
Q9Y6W8
UniProt Secondary Accession #
Q8N6W8
UniProt Related Accession #
Q9Y6W8
GenBank Nucleotide #
NM_012092.3
NCBI Official Full Name
inducible T-cell costimulator
NCBI Official Synonym Full Names
inducible T cell costimulator
NCBI Official Symbol
ICOS
NCBI Official Synonym Symbols
AILIM; CD278; CVID1
UniProt Protein Name
Inducible T-cell costimulator
UniProt Synonym Protein Names
Activation-inducible lymphocyte immunomediatory molecule; CD_antigen: CD278
Protein Family
Inducible T-cell costimulator
UniProt Gene Name
ICOS
UniProt Synonym Gene Names
AILIM
NCBI Protein Information
inducible T-cell costimulator

NCBI Summary for ICOS

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

UniProt Comments for ICOS

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes ().
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Research Articles on ICOS

1. OX40 and ICOS act in a cooperative, nonredundant manner to maximize and prolong the T follicular helper cell response that is generated after acute virus infection

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ICOS antibody

 Reference Product  PubMed Publications
 CTLA4 antibody  >202 publications with ICOS and CTLA4
 ICOSLG antibody  >60 publications with ICOS and ICOSLG
 RC3H1 antibody  >17 publications with ICOS and RC3H1
 PIK3CD antibody  >5 publications with ICOS and PIK3CD
 CD40LG antibody  >5 publications with ICOS and CD40LG
 PIK3CB antibody  >2 publications with ICOS and PIK3CB
 PIK3CG antibody  >1 publications with ICOS and PIK3CG

Pathways associated with anti-ICOS antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-ICOS antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >150 publications with ICOS and Inflammation
 Death Antibodies  >109 publications with ICOS and Death
 Disease Models, Animal Antibodies  >108 publications with ICOS and Disease Models, Animal
 Necrosis Antibodies  >87 publications with ICOS and Necrosis
 Respiratory Tract Diseases Antibodies  >78 publications with ICOS and Respiratory Tract Diseases
 Hypersensitivity Antibodies  >68 publications with ICOS and Hypersensitivity
 Lung Diseases Antibodies  >59 publications with ICOS and Lung Diseases
 Common Variable Immunodeficiency Antibodies  >45 publications with ICOS and Common Variable Immunodeficiency
 Respiratory Hypersensitivity Antibodies  >44 publications with ICOS and Respiratory Hypersensitivity
 Liver Diseases Antibodies  >20 publications with ICOS and Liver Diseases

Organs/Tissues associated with anti-ICOS antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >817 publications with ICOS and Blood
 Lung Antibodies  >62 publications with ICOS and Lung
 Heart Antibodies  >41 publications with ICOS and Heart
 Brain Antibodies  >29 publications with ICOS and Brain
 Thymus Antibodies  >21 publications with ICOS and Thymus
 Pharynx Antibodies  >9 publications with ICOS and Pharynx
 Thyroid Antibodies  >8 publications with ICOS and Thyroid
 Uterus Antibodies  >2 publications with ICOS and Uterus
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