Rabbit Anti-CYBB Polyclonal Antibody – Cat# MBS1750372

Figure 1. Western blot analysis of NOX2 using anti-NOX2 antibody (MBS1750372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG cell lysate,
Lane 2: human Hela cell lysate,
Lane 3: human HepG2 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.

NOX2/Gp91phox (CYBB) Antibody – Rabbit Polyclonal

Name: CYBB Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750372
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750372

Description: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NOX2/gp91phox (CYBB), Polyclonal Antibody

Full Product Name

Anti-NOX2/gp91phox antibody

Product Gene Name

anti-CYBB antibody

Product Synonym Names

Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome; CYBB; NOX2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

65,336 Da

OMIM

X04011 mRNA

3D Structure

ModBase 3D Structure for P04839

Immunogen

E Coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

Subcellular Localization

Cell membrane; Multi-pass membrane protein.

Tissue Specificity

Detected in neutrophils (at protein level).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CYBB antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-CYBB antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-CYBB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYBB purchase

MBS1750372 is an antibody targeting CYBB. Also known as Anti-NOX2/gp91phox antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYBB. It may not necessarily be applicable to this product.

NCBI GI #

6996021

NCBI GeneID

1536

NCBI Accession #

NP_000388.2

UniProt Primary Accession #

P04839

UniProt Secondary Accession #

Q2PP16; A8K138

UniProt Related Accession #

P04839

GenBank Nucleotide #

NM_000397.3

NCBI Official Full Name

cytochrome b-245 heavy chain

NCBI Official Synonym Full Names

cytochrome b-245 beta chain

NCBI Official Symbol

CYBB

NCBI Official Synonym Symbols

CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX

UniProt Protein Name

Cytochrome b-245 heavy chain

UniProt Synonym Protein Names

CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome

Protein Family

Cytochrome

UniProt Gene Name

CYBB

UniProt Synonym Gene Names

NOX2; Cytochrome b558 subunit beta

NCBI Protein Information

cytochrome b-245 heavy chain

NCBI Summary for CYBB

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

UniProt Comments for CYBB

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

Research Articles on CYBB

1. Nox2 activity efficiently contributes to the mechanism of oxidative stress-induced increase in premature aging conferred by doxorubicin. The importance of modulation of Nox2 in human EPCs could reveal a useful tool to restore endothelial progenitor cells physiological function and properties.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CYBB antibody

Reference Product	PubMed Publications
CYBA antibody	>119 publications with CYBB and CYBA
RAC1 antibody	>83 publications with CYBB and RAC1
NCF1 antibody	>40 publications with CYBB and NCF1
NCF2 antibody	>31 publications with CYBB and NCF2
NCF4 antibody	>9 publications with CYBB and NCF4
MMP9 antibody	>4 publications with CYBB and MMP9
VCAM1 antibody	>1 publications with CYBB and VCAM1

Pathways associated with anti-CYBB antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing-Cross Presentation Pathway antibodies	Antigen Processing-Cross Presentation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway antibodies	Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway antibodies	Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-CYBB antibody

Disease Name	Pubmed Publications
Granulomatous Disease, Chronic Antibodies	>298 publications with CYBB and Granulomatous Disease, Chronic
Cardiovascular Diseases Antibodies	>288 publications with CYBB and Cardiovascular Diseases
Inflammation Antibodies	>225 publications with CYBB and Inflammation
Disease Models, Animal Antibodies	>172 publications with CYBB and Disease Models, Animal
Heart Diseases Antibodies	>118 publications with CYBB and Heart Diseases
Hypertension Antibodies	>117 publications with CYBB and Hypertension
Necrosis Antibodies	>107 publications with CYBB and Necrosis
Reperfusion Injury Antibodies	>61 publications with CYBB and Reperfusion Injury
Fibrosis Antibodies	>57 publications with CYBB and Fibrosis
Atherosclerosis Antibodies	>56 publications with CYBB and Atherosclerosis

Organs/Tissues associated with anti-CYBB antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>608 publications with CYBB and Blood
Vascular Antibodies	>324 publications with CYBB and Vascular
Brain Antibodies	>119 publications with CYBB and Brain
Lung Antibodies	>110 publications with CYBB and Lung
Kidney Antibodies	>92 publications with CYBB and Kidney
Bone Antibodies	>78 publications with CYBB and Bone
Bone Marrow Antibodies	>71 publications with CYBB and Bone Marrow
Liver Antibodies	>44 publications with CYBB and Liver
Eye Antibodies	>25 publications with CYBB and Eye
Connective Tissue Antibodies	>21 publications with CYBB and Connective Tissue