Rabbit Anti-BIRC5 Polyclonal Antibody – Cat# MBS1750383

Figure 1. Western blot analysis of Survivin using anti- Survivin antibody (MBS1750383).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.

Survivin (BIRC5) Antibody – Rabbit Polyclonal

Name: BIRC5 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750383
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750383

Description: Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Survivin (BIRC5), Polyclonal Antibody

Full Product Name

Anti-Survivin Picoband Antibody

Product Gene Name

anti-BIRC5 antibody

Product Synonym Names

Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; API4; IAP4

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

16389 MW

OMIM

U75285 Genomic DNA

3D Structure

ModBase 3D Structure for O15392

Immunogen

E Coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively.

Subcellular Localization

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export.

Tissue Specificity

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-BIRC5 antibody

Cell Biology; Apoptosis; Intracellular; Survivin/IAPS; Epigenetics and Nuclear Signaling; Chromosome Structure; Chromosome; Scaffold Proteins; Cell Cycle; Nuclear; Transcription; Transcription Factors; Cell Biology; Cell Division; Other Cell Division Anti

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-BIRC5 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-BIRC5 antibody

ELISA: 0.1-0.5mug/ml
WB: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-BIRC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BIRC5 purchase

MBS1750383 is an antibody targeting BIRC5. Also known as Anti-Survivin Picoband Antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BIRC5. It may not necessarily be applicable to this product.

NCBI GI #

59859880

NCBI GeneID

332

NCBI Accession #

NP_001012270.1

UniProt Primary Accession #

O15392

UniProt Secondary Accession #

Q2I3N8; Q4VGX0; Q53F61; Q5MGC6; Q6FHL2; Q75SP2; Q9P2W8; A2SUH6; B2R4R1

UniProt Related Accession #

O15392

GenBank Nucleotide #

NM_001012270.1

NCBI Official Full Name

baculoviral IAP repeat-containing protein 5 isoform 2

NCBI Official Synonym Full Names

baculoviral IAP repeat containing 5

NCBI Official Symbol

BIRC5

NCBI Official Synonym Symbols

API4; EPR-1

UniProt Protein Name

Baculoviral IAP repeat-containing protein 5

UniProt Synonym Protein Names

Apoptosis inhibitor 4; Apoptosis inhibitor survivin

Protein Family

Baculoviral IAP repeat-containing protein

UniProt Gene Name

BIRC5

UniProt Synonym Gene Names

API4; IAP4

NCBI Protein Information

baculoviral IAP repeat-containing protein 5

NCBI Summary for BIRC5

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

UniProt Comments for BIRC5

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Research Articles on BIRC5

1. increased expression of both DNA methytransferase I (DNMT1) and Survivin was observed and significantly correlated with the reduced miR-203 in NSCLC

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BIRC5 antibody

Reference Product	PubMed Publications
CASP3 antibody	>419 publications with BIRC5 and CASP3
XIAP antibody	>167 publications with BIRC5 and XIAP
CASP9 antibody	>94 publications with BIRC5 and CASP9
DIABLO antibody	>52 publications with BIRC5 and DIABLO
INCENP antibody	>45 publications with BIRC5 and INCENP
CASP7 antibody	>41 publications with BIRC5 and CASP7
CDK4 antibody	>23 publications with BIRC5 and CDK4
CDK1 antibody	>23 publications with BIRC5 and CDK1
CDCA8 antibody	>22 publications with BIRC5 and CDCA8
E2F1 antibody	>20 publications with BIRC5 and E2F1

Pathways associated with anti-BIRC5 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Aurora A Signaling Pathway antibodies	Aurora A Signaling Pathway Diagram
Aurora B Signaling Pathway antibodies	Aurora B Signaling Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Colorectal Cancer Pathway antibodies	Colorectal Cancer Pathway Diagram
Colorectal Cancer Pathway antibodies	Colorectal Cancer Pathway Diagram
FOXM1 Transcription Factor Network Pathway antibodies	FOXM1 Transcription Factor Network Pathway Diagram
Hepatitis B Pathway antibodies	Hepatitis B Pathway Diagram

Diseases associated with anti-BIRC5 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2403 publications with BIRC5 and Neoplasms
Adenocarcinoma Antibodies	>390 publications with BIRC5 and Adenocarcinoma
Gastrointestinal Diseases Antibodies	>363 publications with BIRC5 and Gastrointestinal Diseases
Breast Neoplasms Antibodies	>264 publications with BIRC5 and Breast Neoplasms
Lung Neoplasms Antibodies	>245 publications with BIRC5 and Lung Neoplasms
Neoplasm Metastasis Antibodies	>230 publications with BIRC5 and Neoplasm Metastasis
Colorectal Neoplasms Antibodies	>211 publications with BIRC5 and Colorectal Neoplasms
Carcinoma, Small Cell Antibodies	>207 publications with BIRC5 and Carcinoma, Small Cell
Carcinoma, Squamous Cell Antibodies	>202 publications with BIRC5 and Carcinoma, Squamous Cell
Neoplasms, Experimental Antibodies	>173 publications with BIRC5 and Neoplasms, Experimental

Organs/Tissues associated with anti-BIRC5 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>288 publications with BIRC5 and Lung
Liver Antibodies	>198 publications with BIRC5 and Liver
Lymph Node Antibodies	>158 publications with BIRC5 and Lymph Node
Prostate Antibodies	>137 publications with BIRC5 and Prostate
Brain Antibodies	>125 publications with BIRC5 and Brain
Stomach Antibodies	>119 publications with BIRC5 and Stomach
Bone Antibodies	>116 publications with BIRC5 and Bone
Skin Antibodies	>101 publications with BIRC5 and Skin
Embryonic Tissue Antibodies	>60 publications with BIRC5 and Embryonic Tissue
Heart Antibodies	>41 publications with BIRC5 and Heart