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ID2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750394

Description: DNA-binding protein inhibitor ID-2 is a protein that in humans is encoded by the ID2 gene. The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a… More details

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Product Overview

Online Datasheet in PDF
Product Name
ID2, Polyclonal Antibody
Full Product Name
Anti-ID2 Picoband Antibody
Product Gene Name
anti-ID2 antibody
Product Synonym Names
DNA-binding protein inhibitor ID-2; Class B basic helix-loop-helix protein 26; bHLHb26; Inhibitor of DNA binding 2; Inhibitor of differentiation 2; ID2; BHLHB26
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
14917 MW
OMIM
D13891 mRNA
3D Structure
ModBase 3D Structure for Q02363
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ID2 (86-120aa VSLHHQRPGQNQASRTPLTTLNTDISILSLQASEF), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Subcellular Localization
Cytoplasm. Nucleus.
Tissue Specificity
Highly expressed in early fetal tissues, including those of the central nervous system.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-ID2 antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; HLH/Leucine Zipper; HLH; Stem Cells; Signaling Pathways; TGF Beta; nuclear
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ID2 antibody
Western Blot (WB)
Application Notes for anti-ID2 antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-ID2 antibody

Figure 1. Western blot analysis of ID2 using anti- ID2 antibody (MBS1750394).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ID2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ID2 at approximately 15KD. The expected band size for ID2 is at 15KD.
Rabbit Anti-ID2 Polyclonal Antibody – Cat# MBS1750394

Other Notes

Small volumes of anti-ID2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ID2 purchase

MBS1750394 is an antibody targeting ID2. Also known as Anti-ID2 Picoband Antibody. Reactive in Human, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ID2. It may not necessarily be applicable to this product.

NCBI GI #
31982933
NCBI GeneID
3398
NCBI Accession #
NP_002157.2
UniProt Primary Accession #
Q02363
UniProt Related Accession #
Q02363
GenBank Nucleotide #
NM_002166.4
NCBI Official Full Name
DNA-binding protein inhibitor ID-2
NCBI Official Synonym Full Names
inhibitor of DNA binding 2
NCBI Official Symbol
ID2
NCBI Official Synonym Symbols
GIG8; ID2A; ID2H; bHLHb26
UniProt Protein Name
DNA-binding protein inhibitor ID-2
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 26; bHLHb26; Inhibitor of DNA binding 2; Inhibitor of differentiation 2
Protein Family
DNA-binding protein inhibitor
UniProt Gene Name
ID2
UniProt Synonym Gene Names
BHLHB26; bHLHb26
NCBI Protein Information
DNA-binding protein inhibitor ID-2

NCBI Summary for ID2

The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]
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UniProt Comments for ID2

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
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Research Articles on ID2

1. ID2 is localized to peritoneal mesothelial and stromal cells of women with and without endometriosis. ID2 mRNA expression is lower in peritoneum adjacent to the endometriosis lesions compared to distal sites

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ID2 antibody

 Reference Product  PubMed Publications
 MYC antibody  >58 publications with ID2 and MYC
 TCF3 antibody  >35 publications with ID2 and TCF3
 BMP4 antibody  >31 publications with ID2 and BMP4
 CTNNB1 antibody  >19 publications with ID2 and CTNNB1
 TCF4 antibody  >12 publications with ID2 and TCF4
 CDK2 antibody  >8 publications with ID2 and CDK2
 RB1 antibody  >3 publications with ID2 and RB1
 PKD2 antibody  >2 publications with ID2 and PKD2

Pathways associated with anti-ID2 antibody

 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-ID2 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >47 publications with ID2 and Carcinoma
 Nervous System Diseases Antibodies  >41 publications with ID2 and Nervous System Diseases
 Skin Diseases Antibodies  >29 publications with ID2 and Skin Diseases
 Cell Transformation, Neoplastic Antibodies  >27 publications with ID2 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >26 publications with ID2 and Adenocarcinoma
 Inflammation Antibodies  >20 publications with ID2 and Inflammation
 Breast Neoplasms Antibodies  >20 publications with ID2 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >19 publications with ID2 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >18 publications with ID2 and Cardiovascular Diseases
 Liver Diseases Antibodies  >16 publications with ID2 and Liver Diseases

Organs/Tissues associated with anti-ID2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >190 publications with ID2 and Blood
 Bone Antibodies  >146 publications with ID2 and Bone
 Brain Antibodies  >79 publications with ID2 and Brain
 Embryonic Tissue Antibodies  >64 publications with ID2 and Embryonic Tissue
 Nerve Antibodies  >61 publications with ID2 and Nerve
 Liver Antibodies  >56 publications with ID2 and Liver
 Bone Marrow Antibodies  >54 publications with ID2 and Bone Marrow
 Vascular Antibodies  >44 publications with ID2 and Vascular
 Heart Antibodies  >33 publications with ID2 and Heart
 Kidney Antibodies  >30 publications with ID2 and Kidney
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