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MMP13 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750395

Description: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
MMP13, Polyclonal Antibody
Full Product Name
Anti-MMP13 Picoband antibody
Product Gene Name
anti-MMP13 antibody
Product Synonym Names
Collagenase 3; Matrix metalloproteinase-13; MMP-13; UMRCASE; Mmp13
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
53,375 Da
3D Structure
ModBase 3D Structure for P23097
Immunogen
E Coli-derived rat MMP13 recombinant protein (Position: Y99-H335).
Subcellular Localization
Secreted, extracellular space, extracellular matrix. Secreted.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-MMP13 antibody

Cardiovascular; Angiogenesis; Adhesion/ECM; Matrix Metalloproteinases; MMP; Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Enzymes; Cancer; Invasion/Microenvironment; MMPS; Cell Biology; Proteolysis/Ubiquitin; Proteolytic Enzymes; Metall
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MMP13 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MMP13 antibody
WB: 0.1-0.5mug/ml
Immunohistochemistry (Paraffin-embedded Section): 0.5-1mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-MMP13 antibody

Figure 1. Western blot analysis of MMP13 using anti-MMP13 antibody (MBS1750395).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat RH35 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54KD. The expected band size for MMP13 is at 54KD.
Rabbit Anti-MMP13 Polyclonal Antibody – Cat# MBS1750395

Immunohistochemistry (IHC) of anti-MMP13 antibody

Figure 2. IHC analysis of MMP13 using anti-MMP13 antibody (MBS1750395).
MMP13 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP13 Antibody (MBS1750395) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-MMP13 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1750395

Immunohistochemistry (IHC) of anti-MMP13 antibody

Figure 3. IHC analysis of MMP13 using anti-MMP13 antibody (MBS1750395).
MMP13 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP13 Antibody (MBS1750395) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-MMP13 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1750395

Immunohistochemistry (IHC) of anti-MMP13 antibody

Figure 4. IHC analysis of MMP13 using anti-MMP13 antibody (MBS1750395).
MMP13 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP13 Antibody (MBS1750395) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-MMP13 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1750395

Immunohistochemistry (IHC) of anti-MMP13 antibody

Figure 5. IHC analysis of MMP13 using anti-MMP13 antibody (MBS1750395).
MMP13 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP13 Antibody (MBS1750395) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-MMP13 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1750395

Other Notes

Small volumes of anti-MMP13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP13 purchase

MBS1750395 is an antibody targeting MMP13. Also known as Anti-MMP13 Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP13. It may not necessarily be applicable to this product.

NCBI GI #
116872
NCBI GeneID
171052
NCBI Accession #
P23097.1
UniProt Primary Accession #
P23097
UniProt Related Accession #
P23097
NCBI Official Full Name
Collagenase 3
NCBI Official Synonym Full Names
matrix metallopeptidase 13
NCBI Official Symbol
Mmp13
UniProt Protein Name
Collagenase 3
UniProt Synonym Protein Names
Matrix metalloproteinase-13; MMP-13; UMRCASE
Protein Family
Collagenase
UniProt Gene Name
Mmp13
UniProt Synonym Gene Names
MMP-13
NCBI Protein Information
collagenase 3

NCBI Summary for MMP13

enzyme that degrades collagen type I [RGD, Feb 2006]

UniProt Comments for MMP13

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().
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Research Articles on MMP13

1. TGF-beta1 stimulates the phosphorylation of Runx2 at three serine amino acids, and this event is required for MMP-13 expression in osteoblastic cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP13 antibody

 Reference Product  PubMed Publications
 Mmp9 antibody  >196 publications with MMP13 and Mmp9
 Timp1 antibody  >75 publications with MMP13 and Timp1
 Mmp14 antibody  >58 publications with MMP13 and Mmp14
 Timp2 antibody  >32 publications with MMP13 and Timp2
 Mmp11 antibody  >14 publications with MMP13 and Mmp11
 Ctsk antibody  >13 publications with MMP13 and Ctsk
 Ctsl1 antibody  >3 publications with MMP13 and Ctsl1
 Col9a2 antibody  >2 publications with MMP13 and Col9a2
 Col18a1 antibody  >2 publications with MMP13 and Col18a1

Pathways associated with anti-MMP13 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram

Organs/Tissues associated with anti-MMP13 antibody

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >246 publications with MMP13 and Connective Tissue
 Brain Antibodies  >26 publications with MMP13 and Brain
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