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EPO Receptor (EPOR) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750400

Description: The erythropoietin receptor (EpoR) is a protein that in humans is encoded by the EPOR gene. This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EPO Receptor (Epor), Polyclonal Antibody
Full Product Name
Anti-EPO Receptor Picoband antibody
Product Gene Name
anti-Epor antibody
Product Synonym Names
Erythropoietin receptor; EPO-R; Epor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
28,798 Da
3D Structure
ModBase 3D Structure for P14753
Immunogen
E Coli-derived mouse EPO Receptor recombinant protein (Position: D32-E225).
Subcellular Localization
Isoform EPOR-F: Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in relatively mature erythroid progenitor cells and in EPO-responsive erythroleukemia cells.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Epor antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-Epor antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-Epor antibody

Figure 1. Western blot analysis of EPO Receptor using anti-EPO Receptor antibody (MBS1750400).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EPO Receptor at approximately 55KD. The expected band size for EPO Receptor is at 55KD.
Rabbit Anti-EPOR Polyclonal Antibody – Cat# MBS1750400

Other Notes

Small volumes of anti-Epor antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Epor purchase

MBS1750400 is an antibody targeting Epor. Also known as Anti-EPO Receptor Picoband antibody. Reactive in Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Epor. It may not necessarily be applicable to this product.

NCBI GI #
116292186
NCBI GeneID
13857
NCBI Accession #
NP_034279.3
UniProt Primary Accession #
P14753
UniProt Secondary Accession #
Q63852
UniProt Related Accession #
P14753
GenBank Nucleotide #
NM_010149.3
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
Epor
UniProt Protein Name
Erythropoietin receptor
Protein Family
Erythropoietin receptor
UniProt Gene Name
Epor
UniProt Synonym Gene Names
EPO-R
NCBI Protein Information
erythropoietin receptor

UniProt Comments for Epor

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Research Articles on Epor

1. CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Epor antibody

 Reference Product  PubMed Publications
 EPO antibody  >616 publications with Epor and EPO
 JAK2 antibody  >162 publications with Epor and JAK2
 KITLG antibody  >93 publications with Epor and KITLG
 KIT antibody  >45 publications with Epor and KIT
 MAPK3 antibody  >15 publications with Epor and MAPK3
 PTPN6 antibody  >13 publications with Epor and PTPN6
 MAPK1 antibody  >13 publications with Epor and MAPK1
 STAT5A antibody  >12 publications with Epor and STAT5A
 PTPN11 antibody  >10 publications with Epor and PTPN11
 GRB2 antibody  >9 publications with Epor and GRB2

Pathways associated with anti-Epor antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram

Diseases associated with anti-Epor antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >164 publications with Epor and Anemia
 Polycythemia Antibodies  >78 publications with Epor and Polycythemia
 Cardiovascular Diseases Antibodies  >70 publications with Epor and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >59 publications with Epor and Disease Models, Animal
 Kidney Diseases Antibodies  >36 publications with Epor and Kidney Diseases
 Breast Neoplasms Antibodies  >32 publications with Epor and Breast Neoplasms
 Inflammation Antibodies  >28 publications with Epor and Inflammation
 Necrosis Antibodies  >28 publications with Epor and Necrosis
 Adenocarcinoma Antibodies  >28 publications with Epor and Adenocarcinoma
 Heart Diseases Antibodies  >19 publications with Epor and Heart Diseases

Organs/Tissues associated with anti-Epor antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >295 publications with Epor and Blood
 Bone Antibodies  >112 publications with Epor and Bone
 Brain Antibodies  >92 publications with Epor and Brain
 Kidney Antibodies  >61 publications with Epor and Kidney
 Liver Antibodies  >60 publications with Epor and Liver
 Heart Antibodies  >36 publications with Epor and Heart
 Embryonic Tissue Antibodies  >28 publications with Epor and Embryonic Tissue
 Lung Antibodies  >21 publications with Epor and Lung
 Eye Antibodies  >20 publications with Epor and Eye
 Skin Antibodies  >10 publications with Epor and Skin
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