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IL1RA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750455

Description: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IL1RA, Polyclonal Antibody
Full Product Name
Anti-IL1RA Picoband Antibody
Product Gene Name
anti-IL1RA antibody
Product Synonym Gene Name
Il1rn
Product Synonym Names
Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; IL1 inhibitor; Il1rn; Il-1ra
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
20274 MW
3D Structure
ModBase 3D Structure for P25085
Immunogen
E Coli-derived mouse IL1RA recombinant protein (Position: R27-Q178). Mouse IL1RA shares 77% and 90.7% amino acid (aa) sequence identity with human and rat IL1RA, respectively.
Subcellular Localization
Isoform 1: Secreted.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-IL1RA antibody

Immunology; Innate Immunity; Cytokines; Interleukins; Kits/Lysates/Other; ELISA Kits; interleukin ELISA Kits; cytokines and cytokine Receptors ELISA Kits
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IL1RA antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL1RA antibody
ELISA: 0.1-0.5mug/ml
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-IL1RA antibody

Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (MBS1750455).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL1RA protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA at approximately 17KD. The expected band size for IL1RA is at 17KD.
Rabbit Anti-IL1RA Polyclonal Antibody – Cat# MBS1750455

Other Notes

Small volumes of anti-IL1RA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL1RA purchase

MBS1750455 is an antibody targeting IL1RA. Also known as Anti-IL1RA Picoband Antibody. Reactive in Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL1RA. It may not necessarily be applicable to this product.

NCBI GI #
89257344
NCBI GeneID
16181
NCBI Accession #
NP_001034790.1
UniProt Primary Accession #
P25085
UniProt Secondary Accession #
O70207
UniProt Related Accession #
P25085
GenBank Nucleotide #
NM_001039701.3
NCBI Official Full Name
interleukin-1 receptor antagonist protein isoform 2
NCBI Official Synonym Full Names
interleukin 1 receptor antagonist
NCBI Official Symbol
Il1rn
NCBI Official Synonym Symbols
IL-1ra; F630041P17Rik
UniProt Protein Name
Interleukin-1 receptor antagonist protein
UniProt Synonym Protein Names
IL1 inhibitor
UniProt Gene Name
Il1rn
UniProt Synonym Gene Names
Il-1ra; IL-1RN; IL-1ra; IRAP
NCBI Protein Information
interleukin-1 receptor antagonist protein

UniProt Comments for IL1RA

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.

Research Articles on IL1RA

1. Findings identify a critical role for IL1Ra in maintaining the natural diversity and composition of intestinal microbiota, and suggest a role for TLR4 in mucosal Th17 cell induction associated with the development of autoimmune disease in mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL1RA antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 IL-1 Signaling Pathway antibodies  IL-1 Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-1 Signaling Pathway antibodies  Interleukin-1 Signaling Pathway Diagram
 Signaling By Interleukins Pathway antibodies  Signaling By Interleukins Pathway Diagram

Diseases associated with anti-IL1RA antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >778 publications with IL1RA and Necrosis
 Inflammation Antibodies  >697 publications with IL1RA and Inflammation
 Arthritis, Rheumatoid Antibodies  >302 publications with IL1RA and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >190 publications with IL1RA and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >165 publications with IL1RA and Disease Models, Animal
 Skin Diseases Antibodies  >126 publications with IL1RA and Skin Diseases
 Lung Diseases Antibodies  >111 publications with IL1RA and Lung Diseases
 Pain Antibodies  >82 publications with IL1RA and Pain
 Kidney Diseases Antibodies  >80 publications with IL1RA and Kidney Diseases
 Heart Diseases Antibodies  >76 publications with IL1RA and Heart Diseases

Organs/Tissues associated with anti-IL1RA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1072 publications with IL1RA and Blood
 Skin Antibodies  >127 publications with IL1RA and Skin
 Bone Marrow Antibodies  >67 publications with IL1RA and Bone Marrow
 Stomach Antibodies  >51 publications with IL1RA and Stomach
 Connective Tissue Antibodies  >50 publications with IL1RA and Connective Tissue
 Spleen Antibodies  >43 publications with IL1RA and Spleen
 Intestine Antibodies  >40 publications with IL1RA and Intestine
 Eye Antibodies  >38 publications with IL1RA and Eye
 Vagina Antibodies  >11 publications with IL1RA and Vagina
 Thymus Antibodies  >7 publications with IL1RA and Thymus
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