Rabbit Anti-TPMT Polyclonal Antibody – Cat# MBS1750463

Figure 1. Western blot analysis of TPMT using anti-TPMT antibody (MBS1750463).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPMT antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TPMT at approximately 28KD. The expected band size for TPMT is at 28KD.

TPMT Antibody – Rabbit Polyclonal

Name: TPMT Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750463
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750463

Description: Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme that in humans is encoded by the TPMT gene. This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TPMT, Polyclonal Antibody

Full Product Name

Anti-TPMT antibody

Product Gene Name

anti-TPMT antibody

Product Synonym Names

Thiopurine S-methyltransferase; Thiopurine methyltransferase; TPMT

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

28,180 Da

OMIM

S62904 mRNA

3D Structure

ModBase 3D Structure for P51580

Immunogen

E Coli-derived human TPMT recombinant protein (Position: M1-K245).

Subcellular Localization

Cytoplasm.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TPMT antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-TPMT antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-TPMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPMT purchase

MBS1750463 is an antibody targeting TPMT. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TPMT. It may not necessarily be applicable to this product.

NCBI GI #

4507653

NCBI GeneID

7172

NCBI Accession #

NP_000358.1

UniProt Primary Accession #

P51580

UniProt Secondary Accession #

O14806; O15423; O15424; O15425; O15426; O15515; O15548; O43213; Q5VUK6; Q9UBE6; Q9UBT8

UniProt Related Accession #

P51580

GenBank Nucleotide #

NM_000367.4

NCBI Official Full Name

thiopurine S-methyltransferase isoform 1

NCBI Official Synonym Full Names

thiopurine S-methyltransferase

NCBI Official Symbol

TPMT

NCBI Official Synonym Symbols

TPMTD

UniProt Protein Name

Thiopurine S-methyltransferase

UniProt Synonym Protein Names

Thiopurine methyltransferase

UniProt Gene Name

TPMT

NCBI Protein Information

thiopurine S-methyltransferase

NCBI Summary for TPMT

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

UniProt Comments for TPMT

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

Research Articles on TPMT

1. This article reviews the thiopurine pharmacogenetics and the methods applied in common practice to evaluate patient's TPMT status. [review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPMT antibody

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway antibodies	Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway Diagram
Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway antibodies	Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway Diagram
Defective GGT1 Causes Glutathionuria (GLUTH) Pathway antibodies	Defective GGT1 Causes Glutathionuria (GLUTH) Pathway Diagram
Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway antibodies	Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway Diagram
Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway antibodies	Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway Diagram
Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway antibodies	Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway Diagram
Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway antibodies	Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway Diagram
Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway antibodies	Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway Diagram
Defective UGT1A1 Causes Hyperbilirubinemia Pathway antibodies	Defective UGT1A1 Causes Hyperbilirubinemia Pathway Diagram

Diseases associated with anti-TPMT antibody

Disease Name	Pubmed Publications
Neutropenia Antibodies	>87 publications with TPMT and Neutropenia
Leukopenia Antibodies	>69 publications with TPMT and Leukopenia
Liver Diseases Antibodies	>43 publications with TPMT and Liver Diseases
Drug-Related Side Effects and Adverse Reactions Antibodies	>37 publications with TPMT and Drug-Related Side Effects and Adverse Reactions
Thrombocytopenia Antibodies	>13 publications with TPMT and Thrombocytopenia
Drug-Induced Liver Injury Antibodies	>11 publications with TPMT and Drug-Induced Liver Injury
Kidney Diseases Antibodies	>10 publications with TPMT and Kidney Diseases
Necrosis Antibodies	>10 publications with TPMT and Necrosis
Drug Hypersensitivity Antibodies	>10 publications with TPMT and Drug Hypersensitivity
Anemia Antibodies	>10 publications with TPMT and Anemia

Organs/Tissues associated with anti-TPMT antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>326 publications with TPMT and Blood
Bone Antibodies	>75 publications with TPMT and Bone
Bone Marrow Antibodies	>74 publications with TPMT and Bone Marrow
Liver Antibodies	>68 publications with TPMT and Liver
Kidney Antibodies	>43 publications with TPMT and Kidney
Skin Antibodies	>10 publications with TPMT and Skin
Heart Antibodies	>9 publications with TPMT and Heart
Lung Antibodies	>4 publications with TPMT and Lung
Intestine Antibodies	>4 publications with TPMT and Intestine
Embryonic Tissue Antibodies	>3 publications with TPMT and Embryonic Tissue