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EGR1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750465

Description: Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2.… More details

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Product Overview

Online Datasheet in PDF
Product Name
Egr1, Polyclonal Antibody
Full Product Name
Anti-Egr1 Picoband antibody
Product Gene Name
anti-Egr1 antibody
Product Synonym Names
Early growth response protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
57,507 Da
OMIM
X52541 mRNA
3D Structure
ModBase 3D Structure for P18146
Immunogen
E Coli-derived human Egr1 recombinant protein (Position: A239-R327).
Subcellular Localization
Nucleus.
Tissue Specificity
Detected in neutrophils (at protein level).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Egr1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-Egr1 antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-Egr1 antibody

Figure 1. Western blot analysis of Egr1 using anti-Egr1 antibody (MBS1750465).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human 22RV1 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Egr1 at approximately 75KD. The expected band size for Egr1 is at 58KD.
Rabbit Anti-EGR1 Polyclonal Antibody – Cat# MBS1750465

Other Notes

Small volumes of anti-Egr1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Egr1 purchase

MBS1750465 is an antibody targeting Egr1. Also known as Anti-Egr1 Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Egr1. It may not necessarily be applicable to this product.

NCBI GI #
4503493
NCBI GeneID
1958
NCBI Accession #
NP_001955.1
UniProt Primary Accession #
P18146
UniProt Related Accession #
P18146
GenBank Nucleotide #
NM_001964.2
NCBI Official Full Name
early growth response protein 1
NCBI Official Synonym Full Names
early growth response 1
NCBI Official Symbol
EGR1
NCBI Official Synonym Symbols
TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
UniProt Protein Name
Early growth response protein 1
UniProt Synonym Protein Names
AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225
Protein Family
Early growth response protein
UniProt Gene Name
EGR1
UniProt Synonym Gene Names
; NGFI-A
NCBI Protein Information
early growth response protein 1

NCBI Summary for Egr1

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]
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UniProt Comments for Egr1

Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary ().
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Research Articles on Egr1

1. Egr-1 upregulation is characteristic of the cardiovascular disease pathogenesis. (Review)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Egr1 antibody

 Reference Product  PubMed Publications
 FOS antibody  >574 publications with Egr1 and FOS
 JUN antibody  >501 publications with Egr1 and JUN
 NAB2 antibody  >46 publications with Egr1 and NAB2
 ELK1 antibody  >44 publications with Egr1 and ELK1
 PTEN antibody  >28 publications with Egr1 and PTEN
 NAB1 antibody  >26 publications with Egr1 and NAB1
 TP53 antibody  >16 publications with Egr1 and TP53
 SNAI1 antibody  >1 publications with Egr1 and SNAI1
 NFATC1 antibody  >1 publications with Egr1 and NFATC1
 NFATC2 antibody  >1 publications with Egr1 and NFATC2

Pathways associated with anti-Egr1 antibody

 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-Egr1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >515 publications with Egr1 and Neoplasms
 Nervous System Diseases Antibodies  >159 publications with Egr1 and Nervous System Diseases
 Inflammation Antibodies  >141 publications with Egr1 and Inflammation
 Cardiovascular Diseases Antibodies  >129 publications with Egr1 and Cardiovascular Diseases
 Necrosis Antibodies  >125 publications with Egr1 and Necrosis
 Brain Diseases Antibodies  >104 publications with Egr1 and Brain Diseases
 Drug Toxicity Antibodies  >79 publications with Egr1 and Drug Toxicity
 Ischemia Antibodies  >67 publications with Egr1 and Ischemia
 Prostatic Neoplasms Antibodies  >57 publications with Egr1 and Prostatic Neoplasms
 Liver Diseases Antibodies  >49 publications with Egr1 and Liver Diseases

Organs/Tissues associated with anti-Egr1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >720 publications with Egr1 and Brain
 Blood Antibodies  >376 publications with Egr1 and Blood
 Nerve Antibodies  >259 publications with Egr1 and Nerve
 Vascular Antibodies  >229 publications with Egr1 and Vascular
 Muscle Antibodies  >187 publications with Egr1 and Muscle
 Lung Antibodies  >122 publications with Egr1 and Lung
 Kidney Antibodies  >120 publications with Egr1 and Kidney
 Liver Antibodies  >114 publications with Egr1 and Liver
 Heart Antibodies  >93 publications with Egr1 and Heart
 Bone Antibodies  >90 publications with Egr1 and Bone
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