Rabbit Anti-CCL20 Polyclonal Antibody – Cat# MBS1750473

Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (MBS1750473).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.

MIP-3 Alpha/CCL20 (CCL20) Antibody – Rabbit Polyclonal

Name: CCL20 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750473
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750473

Description: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MIP-3 Alpha/CCL20, Polyclonal Antibody

Full Product Name

Anti-MIP-3 Alpha/CCL20 Picoband Antibody

Product Gene Name

anti-Ccl20 antibody

Product Synonym Names

C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20; Ccl20; Larc; Scya20

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

10,755 Da

3D Structure

ModBase 3D Structure for O89093

Immunogen

E Coli-derived mouse MIP-3 Alpha/CCL20 recombinant protein (Position: A28-M97).

Subcellular Localization

Secreted

Tissue Specificity

Thymic medulla (at protein level). Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues. Expressed by IL17 producing helper T-cells (Th17).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-Ccl20 antibody

Immunology; Innate Immunity; Macrophage/Inflamm.; Immunology; Chemokines; beta Chemokines (cc); Signal Transduction; Signaling Pathway; G Protein Signaling; gpcr; Kits/Lysates/Other; ELISA Kits; cytokines and cytokine Receptors ELISA Kits

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Ccl20 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-Ccl20 antibody

WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml

Other Notes

Small volumes of anti-Ccl20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ccl20 purchase

MBS1750473 is an antibody targeting Ccl20. Also known as Anti-MIP-3 Alpha/CCL20 Picoband Antibody. Reactive in Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ccl20. It may not necessarily be applicable to this product.

NCBI GI #

229092240

NCBI GeneID

20297

NCBI Accession #

NP_001153210.1

UniProt Primary Accession #

O89093

UniProt Secondary Accession #

Q9Z1X3

UniProt Related Accession #

O89093

GenBank Nucleotide #

NM_001159738.1

NCBI Official Full Name

C-C motif chemokine 20 isoform 2

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 20

NCBI Official Symbol

Ccl20

NCBI Official Synonym Symbols

CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1

UniProt Protein Name

C-C motif chemokine 20

UniProt Synonym Protein Names

Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20

Protein Family

C-C motif chemokine

UniProt Gene Name

Ccl20

UniProt Synonym Gene Names

Larc; Scya20; MIP-3-alpha

NCBI Protein Information

C-C motif chemokine 20

UniProt Comments for Ccl20

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9862452, PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases (PubMed:21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (). Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation (PubMed:19050256). Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (PubMed:24638065). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (PubMed:10064080).

Research Articles on Ccl20

1. these data indicate that CCL20/CCR6 signaling may play an important role in regulating bone mass accrual, potentially by modulating osteoblast maturation, survival, and the recruitment of osteoblast-supporting cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Ccl20 antibody

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
EBV LMP1 Signaling Pathway antibodies	EBV LMP1 Signaling Pathway Diagram

Diseases associated with anti-Ccl20 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>382 publications with Ccl20 and Inflammation
Necrosis Antibodies	>208 publications with Ccl20 and Necrosis
Skin Diseases Antibodies	>102 publications with Ccl20 and Skin Diseases
Carcinoma Antibodies	>88 publications with Ccl20 and Carcinoma
Lung Diseases Antibodies	>77 publications with Ccl20 and Lung Diseases
Hypersensitivity Antibodies	>57 publications with Ccl20 and Hypersensitivity
Liver Diseases Antibodies	>45 publications with Ccl20 and Liver Diseases
Cardiovascular Diseases Antibodies	>36 publications with Ccl20 and Cardiovascular Diseases
Neoplasms, Experimental Antibodies	>35 publications with Ccl20 and Neoplasms, Experimental
Fibrosis Antibodies	>26 publications with Ccl20 and Fibrosis

Organs/Tissues associated with anti-Ccl20 antibody

Organ/Tissue Name	Pubmed Publications
Thymus Antibodies	>13 publications with Ccl20 and Thymus
Mammary Gland Antibodies	>4 publications with Ccl20 and Mammary Gland
Testis Antibodies	>1 publications with Ccl20 and Testis