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CCR4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750475

CCR4, C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene. CCR4 has also recently been designated CD194 (cluster of differentiation 194). The protein encoded by this gene belongs to the G protein-coupled receptor family. It… More details

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Product Overview

Online Datasheet in PDF
Product Name
CCR4, Polyclonal Antibody
Full Product Name
Anti-CCR4 Picoband Antibody
Product Gene Name
anti-CCR4 antibody
Product Synonym Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194; CCR4; CMKBR4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
X85740 mRNA
3D Structure
ModBase 3D Structure for P51679
Immunogen
A synthetic peptide corresponding to a sequence of human CCR4 (LVELEVLQDCTFERYLDYAIQATETLAFVH).
Subcellular Localization
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CCR4 antibody
Western Blot (WB)
Application Notes for anti-CCR4 antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-CCR4 antibody

Figure 1. Western blot analysis of CCR4 using anti-CCR4 antibody (MBS1750475).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR4 at approximately 41KD. The expected band size for CCR4 is at 41KD.
Rabbit Anti-CCR4 Polyclonal Antibody – Cat# MBS1750475

Other Notes

Small volumes of anti-CCR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCR4 purchase

MBS1750475 is an antibody targeting CCR4. Also known as Anti-CCR4 Picoband Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CCR4. It may not necessarily be applicable to this product.

NCBI GI #
5031627
NCBI GeneID
1233
NCBI Accession #
NP_005499.1
UniProt Primary Accession #
P51679
UniProt Secondary Accession #
Q9ULY6; Q9ULY7
UniProt Related Accession #
P51679
GenBank Nucleotide #
NM_005508.4
NCBI Official Full Name
C-C chemokine receptor type 4
NCBI Official Synonym Full Names
C-C motif chemokine receptor 4
NCBI Official Symbol
CCR4
NCBI Official Synonym Symbols
CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
UniProt Protein Name
C-C chemokine receptor type 4
UniProt Synonym Protein Names
K5-5; CD_antigen: CD194
Protein Family
C-C chemokine receptor
UniProt Gene Name
CCR4
UniProt Synonym Gene Names
CMKBR4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4
NCBI Protein Information
C-C chemokine receptor type 4

NCBI Summary for CCR4

The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]
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UniProt Comments for CCR4

High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
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Research Articles on CCR4

1. monocyte chemoattractant protein-1 (MCP-1), an endogenous CCR4-binding ligand, was specifically upregulated in the head and neck squamous cell carcinomamicroenvironment compared to the other four CCR4-binding ligands

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCR4 antibody

 Reference Product  PubMed Publications
 CCL17 antibody  >226 publications with CCR4 and CCL17
 CCL22 antibody  >198 publications with CCR4 and CCL22
 CCL5 antibody  >83 publications with CCR4 and CCL5
 CCL3 antibody  >43 publications with CCR4 and CCL3
 ADRBK1 antibody  >2 publications with CCR4 and ADRBK1
 ADRBK2 antibody  >1 publications with CCR4 and ADRBK2

Pathways associated with anti-CCR4 antibody

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-CCR4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >239 publications with CCR4 and Inflammation
 Neoplasms Antibodies  >187 publications with CCR4 and Neoplasms
 Lung Diseases Antibodies  >119 publications with CCR4 and Lung Diseases
 Necrosis Antibodies  >77 publications with CCR4 and Necrosis
 Drug Hypersensitivity Antibodies  >33 publications with CCR4 and Drug Hypersensitivity
 Carcinoma Antibodies  >32 publications with CCR4 and Carcinoma
 Fibrosis Antibodies  >26 publications with CCR4 and Fibrosis
 Heart Diseases Antibodies  >16 publications with CCR4 and Heart Diseases
 Neoplasms, Experimental Antibodies  >16 publications with CCR4 and Neoplasms, Experimental
 Liver Diseases Antibodies  >16 publications with CCR4 and Liver Diseases

Organs/Tissues associated with anti-CCR4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >759 publications with CCR4 and Blood
 Thymus Antibodies  >148 publications with CCR4 and Thymus
 Lung Antibodies  >128 publications with CCR4 and Lung
 Brain Antibodies  >25 publications with CCR4 and Brain
 Adipose Tissue Antibodies  >7 publications with CCR4 and Adipose Tissue
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