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IGFBP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750516

Description: IGFBP1, Insulin-like growth factor-binding protein 1, also known as placental protein 12 (PP12), is a protein that in humans is encoded by the IGFBP1 gene. The IGFBP1 gene has 4 exons and spans 5.9 kb. And the IGFBP1 gene is localized to 7p13-p12… More details

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Product Overview

Online Datasheet in PDF
Product Name
IGFBP1, Polyclonal Antibody
Full Product Name
Anti-IGFBP1 Picoband Antibody
Product Gene Name
anti-IGFBP1 antibody
Product Synonym Names
Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; Placental protein 12; PP12; IGFBP1; IBP1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
27904 MW
OMIM
Y00856 mRNA
3D Structure
ModBase 3D Structure for P08833
Immunogen
E Coli-derived human IGFBP1 recombinant protein (Position: A69-N259). Human IGFBP1 shares 67.8% and 69.3% amino acid (aa) sequence identity with mouse and rat IGFBP1, respectively.
Subcellular Localization
Secreted.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-IGFBP1 antibody

Signal Transduction; Growth Factors/Hormones; insulin/insulin-like; Cancer; Growth Factors; insulin and insulin-like; Cardiovascular; Atherosclerosis; Diabetes Associated; Kits/Lysates/Other; ELISA Kits; growth factors and Hormones ELISA Kits
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IGFBP1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-IGFBP1 antibody
Immunohistochemistry (Paraffin-embedded Section): 0.5-1mug/ml (By Heat)
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-IGFBP1 antibody

Figure 1. Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (MBS1750516).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30KD. The expected band size for IGFBP1 is at 30KD.
Rabbit Anti-IGFBP1 Polyclonal Antibody – Cat# MBS1750516

Immunohistochemistry (IHC) of anti-IGFBP1 antibody

Figure 2. IHC analysis of IGFBP1 using anti- IGFBP1 antibody (MBS1750516).
IGFBP1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- IGFBP1 Antibody (MBS1750516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-IGFBP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1750516

Other Notes

Small volumes of anti-IGFBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGFBP1 purchase

MBS1750516 is an antibody targeting IGFBP1. Also known as Anti-IGFBP1 Picoband Antibody. Reactive in Human
No cross reactivity with other proteins. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGFBP1. It may not necessarily be applicable to this product.

NCBI GI #
4504615
NCBI GeneID
3484
NCBI Accession #
NP_000587.1
UniProt Primary Accession #
P08833
UniProt Secondary Accession #
Q8IYP5; A4D2F4; D3DVL9
UniProt Related Accession #
P08833
GenBank Nucleotide #
NM_000596.3
NCBI Official Full Name
insulin-like growth factor-binding protein 1
NCBI Official Synonym Full Names
insulin like growth factor binding protein 1
NCBI Official Symbol
IGFBP1
NCBI Official Synonym Symbols
AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
UniProt Protein Name
Insulin-like growth factor-binding protein 1
UniProt Synonym Protein Names
Placental protein 12; PP12
Protein Family
Insulin-like growth factor-binding protein
UniProt Gene Name
IGFBP1
UniProt Synonym Gene Names
IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
NCBI Protein Information
insulin-like growth factor-binding protein 1

NCBI Summary for IGFBP1

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients. [provided by RefSeq, Aug 2017]
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UniProt Comments for IGFBP1

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Research Articles on IGFBP1

1. NR4A modulates the decidualization of hESCs by upregulating prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1) expression and transformation in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IGFBP1 antibody

 Reference Product  PubMed Publications
 IGFBP4 antibody  >146 publications with IGFBP1 and IGFBP4
 IGF1 antibody  >72 publications with IGFBP1 and IGF1
 IGF2 antibody  >33 publications with IGFBP1 and IGF2
 IL6 antibody  >10 publications with IGFBP1 and IL6
 FOXA2 antibody  >8 publications with IGFBP1 and FOXA2
 INS antibody  >6 publications with IGFBP1 and INS
 ATF4 antibody  >3 publications with IGFBP1 and ATF4
 HIF1A antibody  >3 publications with IGFBP1 and HIF1A
 NR3C1 antibody  >1 publications with IGFBP1 and NR3C1

Pathways associated with anti-IGFBP1 antibody

 Products by Pathway  Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram

Diseases associated with anti-IGFBP1 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >131 publications with IGFBP1 and Liver Diseases
 Cardiovascular Diseases Antibodies  >124 publications with IGFBP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >91 publications with IGFBP1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >86 publications with IGFBP1 and Breast Neoplasms
 Kidney Diseases Antibodies  >83 publications with IGFBP1 and Kidney Diseases
 Liver Neoplasms Antibodies  >81 publications with IGFBP1 and Liver Neoplasms
 Adenocarcinoma Antibodies  >80 publications with IGFBP1 and Adenocarcinoma
 Brain Diseases Antibodies  >76 publications with IGFBP1 and Brain Diseases
 Necrosis Antibodies  >65 publications with IGFBP1 and Necrosis
 Inflammation Antibodies  >63 publications with IGFBP1 and Inflammation

Organs/Tissues associated with anti-IGFBP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1333 publications with IGFBP1 and Blood
 Liver Antibodies  >453 publications with IGFBP1 and Liver
 Uterus Antibodies  >330 publications with IGFBP1 and Uterus
 Placenta Antibodies  >241 publications with IGFBP1 and Placenta
 Embryonic Tissue Antibodies  >132 publications with IGFBP1 and Embryonic Tissue
 Bone Antibodies  >98 publications with IGFBP1 and Bone
 Brain Antibodies  >87 publications with IGFBP1 and Brain
 Lung Antibodies  >46 publications with IGFBP1 and Lung
 Skin Antibodies  >32 publications with IGFBP1 and Skin
 Pancreas Antibodies  >31 publications with IGFBP1 and Pancreas
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