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NFAT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750526

Description: NFATC2(Nuclear factor of activated T-cells, cytoplasmic 2), also known as NFATP or the 'preexisting component' of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T… More details

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Product Overview

Online Datasheet in PDF
Product Name
NFAT1, Polyclonal Antibody
Full Product Name
Anti-NFAT1 Picoband Antibody
Product Gene Name
anti-NFAT1 antibody
Product Synonym Gene Name
NFATC2
Product Synonym Names
Nuclear factor of activated T-cells; cytoplasmic 2; NF-ATc2; NFATc2; NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1; NFATC2; NFAT1; NFATP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
76,700 Da
OMIM
U43341 mRNA
3D Structure
ModBase 3D Structure for Q13469
Immunogen
E Coli-derived human NFAT1 recombinant protein (Position: Q594-H676).
Subcellular Localization
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Tissue Specificity
Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-NFAT1 antibody

Signal Transduction; Signaling Pathway; Nuclear Signaling; nfats; Immunology; Innate Immunity; Cytokines; Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; Adaptive Immunity; Regulatory T Cells
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NFAT1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NFAT1 antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-NFAT1 antibody

Figure 1. Western blot analysis of NFAT1 using anti-NFAT1 antibody (MBS1750526).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT1 at approximately 100KD. The expected band size for NFAT1 is at 100KD.
Rabbit Anti-NFAT1 Polyclonal Antibody – Cat# MBS1750526

Other Notes

Small volumes of anti-NFAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFAT1 purchase

MBS1750526 is an antibody targeting NFAT1. Also known as Anti-NFAT1 Picoband Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFAT1. It may not necessarily be applicable to this product.

NCBI GI #
209862843
NCBI GeneID
4773
NCBI Accession #
NP_001129493.1
UniProt Primary Accession #
Q13469
UniProt Secondary Accession #
Q13468; Q5TFW7; Q5TFW8; Q9NPX6; Q9NQH3; Q9UJR2; B5B2N8; B5B2N9; B5B2P0; B5B2P2; B5B2P3
UniProt Related Accession #
Q13469
GenBank Nucleotide #
NM_001136021.2
NCBI Official Full Name
nuclear factor of activated T-cells, cytoplasmic 2 isoform D
NCBI Official Synonym Full Names
nuclear factor of activated T cells 2
NCBI Official Symbol
NFATC2
NCBI Official Synonym Symbols
NFAT1; NFATP
UniProt Protein Name
Nuclear factor of activated T-cells, cytoplasmic 2
UniProt Synonym Protein Names
NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1
Protein Family
Nuclear factor of activated T-cells
UniProt Gene Name
NFATC2
UniProt Synonym Gene Names
NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
NCBI Protein Information
nuclear factor of activated T-cells, cytoplasmic 2

NCBI Summary for NFAT1

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
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UniProt Comments for NFAT1

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

Research Articles on NFAT1

1. An interaction of NFAT1 and the beta-catenin pathway, validate lysophosphatidic acid as an in vivo activator of beta-catenin-dependent transcription during allograft fibrogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NFAT1 antibody

 Reference Product  PubMed Publications
 JUN antibody  >49 publications with NFAT1 and JUN
 FOS antibody  >21 publications with NFAT1 and FOS
 FOXP3 antibody  >20 publications with NFAT1 and FOXP3
 IL2 antibody  >6 publications with NFAT1 and IL2
 EGR1 antibody  >5 publications with NFAT1 and EGR1
 IL4 antibody  >4 publications with NFAT1 and IL4
 IRF4 antibody  >3 publications with NFAT1 and IRF4
 MAPK8 antibody  >2 publications with NFAT1 and MAPK8
 MEF2D antibody  >1 publications with NFAT1 and MEF2D

Pathways associated with anti-NFAT1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 CLEC7A (Dectin-1) Induces NFAT Activation Pathway antibodies  CLEC7A (Dectin-1) Induces NFAT Activation Pathway Diagram
 CLEC7A (Dectin-1) Signaling Pathway antibodies  CLEC7A (Dectin-1) Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram

Diseases associated with anti-NFAT1 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >49 publications with NFAT1 and Immune System Diseases
 Necrosis Antibodies  >29 publications with NFAT1 and Necrosis
 Inflammation Antibodies  >29 publications with NFAT1 and Inflammation
 Disease Models, Animal Antibodies  >23 publications with NFAT1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >18 publications with NFAT1 and Nervous System Diseases
 Lung Diseases Antibodies  >15 publications with NFAT1 and Lung Diseases
 Hypertrophy Antibodies  >15 publications with NFAT1 and Hypertrophy
 Brain Diseases Antibodies  >12 publications with NFAT1 and Brain Diseases
 Hypertension Antibodies  >11 publications with NFAT1 and Hypertension
 Cardiovascular Diseases Antibodies  >10 publications with NFAT1 and Cardiovascular Diseases

Organs/Tissues associated with anti-NFAT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >211 publications with NFAT1 and Blood
 Lung Antibodies  >19 publications with NFAT1 and Lung
 Heart Antibodies  >18 publications with NFAT1 and Heart
 Spleen Antibodies  >17 publications with NFAT1 and Spleen
 Brain Antibodies  >16 publications with NFAT1 and Brain
 Skin Antibodies  >13 publications with NFAT1 and Skin
 Lymph Node Antibodies  >11 publications with NFAT1 and Lymph Node
 Lymph Antibodies  >10 publications with NFAT1 and Lymph
 Thymus Antibodies  >8 publications with NFAT1 and Thymus
 Embryonic Tissue Antibodies  >4 publications with NFAT1 and Embryonic Tissue
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