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HDAC4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750527

Description: Histone deacetylase 4, also known as HDAC4, is a protein that in humans is encoded by the HDAC4 gene. It is mapped to 2q37.3. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
HDAC4/Histone Deacetylase 4, Polyclonal Antibody
Full Product Name
Anti-HDAC4/Histone Deacetylase 4 Picoband Antibody
Product Gene Name
anti-HDAC4 antibody
Product Synonym Names
Histone deacetylase 4; HD4; 3.5.1.98; HDAC4; KIAA0288
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
119040 MW
OMIM
AF132607 mRNA
3D Structure
ModBase 3D Structure for P56524
Immunogen
E Coli-derived human HDAC4 recombinant protein (Position: Q4-Q213). Human HDAC4 shares 98.6% and 98.1% amino acid (aa) sequence identity with mouse and rat HDAC4, respectively.
Subcellular Localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.
Tissue Specificity
Ubiquitous.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-HDAC4 antibody

Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; Acetylation; Stem Cells; Signaling Pathways; wnt; HDACS; Epigenetics and Nuclear Signaling; class ii/hda1 class; Cardiovascular; Heart; Hypertrophy
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HDAC4 antibody
Western Blot (WB)
Application Notes for anti-HDAC4 antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-HDAC4 antibody

Figure 1. Western blot analysis of HDAC4 using anti-HDAC4 antibody (MBS1750527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC4 at approximately 106KD. The expected band size for HDAC4 is at 106KD.
Rabbit Anti-HDAC4 Polyclonal Antibody – Cat# MBS1750527

Other Notes

Small volumes of anti-HDAC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HDAC4 purchase

MBS1750527 is an antibody targeting HDAC4. Also known as Anti-HDAC4/Histone Deacetylase 4 Picoband Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HDAC4. It may not necessarily be applicable to this product.

NCBI GI #
153085395
NCBI GeneID
9759
NCBI Accession #
NP_006028.2
UniProt Primary Accession #
P56524
UniProt Secondary Accession #
Q86YH7; Q9UND6; E9PGB9; F5GX36
UniProt Related Accession #
P56524
GenBank Nucleotide #
NM_006037.3
NCBI Official Full Name
histone deacetylase 4
NCBI Official Synonym Full Names
histone deacetylase 4
NCBI Official Symbol
HDAC4
NCBI Official Synonym Symbols
HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
UniProt Protein Name
Histone deacetylase 4
UniProt Gene Name
HDAC4
UniProt Synonym Gene Names
KIAA0288; HD4
NCBI Protein Information
histone deacetylase 4

NCBI Summary for HDAC4

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
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UniProt Comments for HDAC4

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256).
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Research Articles on HDAC4

1. HDAC4 regulates thimerosal-induced cell death in neurons and that treatment with MC1568 prevents thimerosal-induced activation of caspase-3 in the rat prefrontal cortex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HDAC4 antibody

 Reference Product  PubMed Publications
 HDAC3 antibody  >53 publications with HDAC4 and HDAC3
 HDAC9 antibody  >28 publications with HDAC4 and HDAC9
 MEF2C antibody  >21 publications with HDAC4 and MEF2C
 RUNX2 antibody  >18 publications with HDAC4 and RUNX2
 MEF2A antibody  >14 publications with HDAC4 and MEF2A
 MEF2D antibody  >10 publications with HDAC4 and MEF2D
 NCOR2 antibody  >5 publications with HDAC4 and NCOR2
 ANKRA2 antibody  >5 publications with HDAC4 and ANKRA2
 SUMO2 antibody  >4 publications with HDAC4 and SUMO2
 CIITA antibody  >3 publications with HDAC4 and CIITA

Pathways associated with anti-HDAC4 antibody

 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram

Diseases associated with anti-HDAC4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >65 publications with HDAC4 and Neoplasms
 Cardiovascular Diseases Antibodies  >26 publications with HDAC4 and Cardiovascular Diseases
 Carcinoma Antibodies  >24 publications with HDAC4 and Carcinoma
 Nervous System Diseases Antibodies  >23 publications with HDAC4 and Nervous System Diseases
 Heart Diseases Antibodies  >17 publications with HDAC4 and Heart Diseases
 Vascular Diseases Antibodies  >15 publications with HDAC4 and Vascular Diseases
 Adenocarcinoma Antibodies  >10 publications with HDAC4 and Adenocarcinoma
 Breast Neoplasms Antibodies  >8 publications with HDAC4 and Breast Neoplasms
 Lymphoma Antibodies  >8 publications with HDAC4 and Lymphoma
 Necrosis Antibodies  >8 publications with HDAC4 and Necrosis

Organs/Tissues associated with anti-HDAC4 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >91 publications with HDAC4 and Muscle
 Brain Antibodies  >41 publications with HDAC4 and Brain
 Heart Antibodies  >38 publications with HDAC4 and Heart
 Bone Antibodies  >29 publications with HDAC4 and Bone
 Blood Antibodies  >23 publications with HDAC4 and Blood
 Embryonic Tissue Antibodies  >18 publications with HDAC4 and Embryonic Tissue
 Liver Antibodies  >12 publications with HDAC4 and Liver
 Lung Antibodies  >11 publications with HDAC4 and Lung
 Prostate Antibodies  >10 publications with HDAC4 and Prostate
 Thyroid Antibodies  >10 publications with HDAC4 and Thyroid
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