Rabbit Anti-PLAUR Polyclonal Antibody – Cat# MBS1750534

Figure 1. Western blot analysis of PLAUR using anti- PLAUR antibody (MBS1750534).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PLAUR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLAUR at approximately 39KD, 55KD. The expected band size for PLAUR is at 37KD.

PLAUR Antibody – Rabbit Polyclonal

Name: PLAUR Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750534
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750534

Description: PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PLAUR/Upar, Polyclonal Antibody

Full Product Name

Anti-PLAUR/Upar Picoband Antibody

Product Gene Name

anti-PLAUR antibody

Product Synonym Names

Urokinase plasminogen activator surface receptor; U-PAR; uPAR; Monocyte activation antigen Mo3; CD87; PLAUR; MO3; UPAR

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

36978 MW

OMIM

X51675 mRNA

3D Structure

ModBase 3D Structure for Q03405

Immunogen

E Coli-derived human PLAUR recombinant protein (Position: E230-S286). Human PLAUR shares 63.2% amino acid (aa) sequence identity with both mouse and rat PLAUR.

Subcellular Localization

Cell membrane. Cell projection, invadopodium membrane. Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner.

Tissue Specificity

Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-PLAUR antibody

Cardiovascular; Blood; Fibrinolysis/Thrombolysis; Immunology; Cell Type Markers; Myeloid Cells; Cancer; Invasion/Microenvironment; ECM; Extracellular Matrix; Tumor Biomarkers; Receptors

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PLAUR antibody

Western Blot (WB)

Application Notes for anti-PLAUR antibody

WB: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-PLAUR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLAUR purchase

MBS1750534 is an antibody targeting PLAUR. Also known as Anti-PLAUR/Upar Picoband Antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PLAUR. It may not necessarily be applicable to this product.

NCBI GI #

53829379

NCBI GeneID

5329

NCBI Accession #

NP_001005376.1

UniProt Primary Accession #

Q03405

UniProt Secondary Accession #

Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409

UniProt Related Accession #

Q03405

GenBank Nucleotide #

NM_001005376.2

NCBI Official Full Name

urokinase plasminogen activator surface receptor isoform 2

NCBI Official Synonym Full Names

plasminogen activator, urokinase receptor

NCBI Official Symbol

PLAUR

NCBI Official Synonym Symbols

CD87; UPAR; URKR; U-PAR

UniProt Protein Name

Urokinase plasminogen activator surface receptor

UniProt Synonym Protein Names

Monocyte activation antigen Mo3; CD_antigen: CD87

Protein Family

Urokinase plasminogen activator surface receptor

UniProt Gene Name

PLAUR

UniProt Synonym Gene Names

MO3; UPAR; U-PAR; uPAR

NCBI Protein Information

urokinase plasminogen activator surface receptor

NCBI Summary for PLAUR

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

UniProt Comments for PLAUR

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Research Articles on PLAUR

1. Two interacting partners of urokinase-type plasminogen activator (uPA) receptor, the cysteine-rich angiogenic inducer 61 (Cyr61) and the Y-box-binding protein 1 (YB-1) were identified and their differential expression demonstrated in the triple-negative breast cancer (TNBC) cells as well as in tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PLAUR antibody

Products by Pathway	Pathway Diagram
Arf6 Downstream Pathway antibodies	Arf6 Downstream Pathway Diagram
Attachment Of GPI Anchor To UPAR Pathway antibodies	Attachment Of GPI Anchor To UPAR Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Dissolution Of Fibrin Clot Pathway antibodies	Dissolution Of Fibrin Clot Pathway Diagram
FGF Signaling Pathway antibodies	FGF Signaling Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway antibodies	Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway Diagram

Diseases associated with anti-PLAUR antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>314 publications with PLAUR and Carcinoma
Neoplasm Invasiveness Antibodies	>262 publications with PLAUR and Neoplasm Invasiveness
Neoplasm Metastasis Antibodies	>240 publications with PLAUR and Neoplasm Metastasis
Breast Neoplasms Antibodies	>165 publications with PLAUR and Breast Neoplasms
Inflammation Antibodies	>116 publications with PLAUR and Inflammation
Lung Diseases Antibodies	>91 publications with PLAUR and Lung Diseases
Cardiovascular Diseases Antibodies	>83 publications with PLAUR and Cardiovascular Diseases
Lung Neoplasms Antibodies	>76 publications with PLAUR and Lung Neoplasms
Neoplasms, Experimental Antibodies	>75 publications with PLAUR and Neoplasms, Experimental
Disease Models, Animal Antibodies	>68 publications with PLAUR and Disease Models, Animal

Organs/Tissues associated with anti-PLAUR antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>508 publications with PLAUR and Blood
Vascular Antibodies	>304 publications with PLAUR and Vascular
Lung Antibodies	>148 publications with PLAUR and Lung
Muscle Antibodies	>99 publications with PLAUR and Muscle
Brain Antibodies	>86 publications with PLAUR and Brain
Liver Antibodies	>84 publications with PLAUR and Liver
Bone Antibodies	>81 publications with PLAUR and Bone
Embryonic Tissue Antibodies	>80 publications with PLAUR and Embryonic Tissue
Kidney Antibodies	>77 publications with PLAUR and Kidney
Skin Antibodies	>68 publications with PLAUR and Skin