Rabbit Anti-Il12B Polyclonal Antibody – Cat# MBS1750584

Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (MBS1750584).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse NIH3T3 cell lysates,
Lane 2: human A549 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.

Il12B Antibody – Rabbit Polyclonal

Name: Il12B Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750584
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750584

Description: Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential… More details

$210 / 0.01 mg +1 FREE 8GB USB

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Testing Data View PDF

Certificate of Analysis (COA) View PDF

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL12B/Il 12, Polyclonal Antibody

Full Product Name

Anti-IL12B/Il 12 Picoband antibody

Product Gene Name

anti-Il12B antibody

Product Synonym Names

Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2; IL12B; NKSF2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

37,169 Da

OMIM

M65272 mRNA

3D Structure

ModBase 3D Structure for P29460

Immunogen

E Coli-derived human IL12B recombinant protein (Position: I23-E253).

Subcellular Localization

Secreted.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-Il12B antibody

Immunology; Innate Immunity; Macrophage/Inflamm.; Immunology; Cytokines; interleukins

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-Il12B antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-Il12B antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-Il12B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Il12B purchase

MBS1750584 is an antibody targeting Il12B. Also known as Anti-IL12B/Il 12 Picoband antibody. Reactive in Human, Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Il12B. It may not necessarily be applicable to this product.

NCBI GI #

24497438

NCBI GeneID

3593

NCBI Accession #

NP_002178.2

UniProt Primary Accession #

P29460

UniProt Related Accession #

P29460

GenBank Nucleotide #

NP_002178.2

NCBI Official Full Name

interleukin-12 subunit beta

NCBI Official Synonym Full Names

interleukin 12B

NCBI Official Symbol

IL12B

NCBI Official Synonym Symbols

CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B

UniProt Protein Name

Interleukin-12 subunit beta

UniProt Synonym Protein Names

Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2

Protein Family

Interleukin

UniProt Gene Name

IL12B

UniProt Synonym Gene Names

NKSF2; IL-12B; CLMF p40; NKSF2

NCBI Protein Information

interleukin-12 subunit beta

NCBI Summary for Il12B

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]

UniProt Comments for Il12B

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Research Articles on Il12B

1. baseline serum IL-12 levels were significantly higher in those pediatric hematopoietic stem cell transplantation recipients who did not develop acute graft versus host disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Il12B antibody

Reference Product	PubMed Publications
IL23R antibody	>65 publications with Il12B and IL23R
IL12A antibody	>43 publications with Il12B and IL12A
IL4 antibody	>37 publications with Il12B and IL4
IL12RB1 antibody	>32 publications with Il12B and IL12RB1
IL6 antibody	>30 publications with Il12B and IL6
IFNG antibody	>27 publications with Il12B and IFNG
IL23A antibody	>19 publications with Il12B and IL23A
CD4 antibody	>14 publications with Il12B and CD4
IL12RB2 antibody	>10 publications with Il12B and IL12RB2
NFKB1 antibody	>2 publications with Il12B and NFKB1

Pathways associated with anti-Il12B antibody

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-Il12B antibody

Disease Name	Pubmed Publications
Psoriasis Antibodies	>55 publications with Il12B and Psoriasis
Inflammation Antibodies	>44 publications with Il12B and Inflammation
Necrosis Antibodies	>34 publications with Il12B and Necrosis
Crohn Disease Antibodies	>26 publications with Il12B and Crohn Disease
Lung Diseases Antibodies	>24 publications with Il12B and Lung Diseases
Arthritis, Psoriatic Antibodies	>19 publications with Il12B and Arthritis, Psoriatic
Colitis, Ulcerative Antibodies	>14 publications with Il12B and Colitis, Ulcerative
Asthma Antibodies	>13 publications with Il12B and Asthma
Liver Diseases Antibodies	>12 publications with Il12B and Liver Diseases
Disease Models, Animal Antibodies	>11 publications with Il12B and Disease Models, Animal

Organs/Tissues associated with anti-Il12B antibody

Organ/Tissue Name	Pubmed Publications
Mixed Antibodies	>2 publications with Il12B and Mixed