Rabbit Anti-VCAM1 Polyclonal Antibody – Cat# MBS1750593

Figure 1. Western blot analysis of VCAM1 using anti-VCAM1 antibody (MBS1750593).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human VCAM1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 95KD. The expected band size for VCAM1 is at 74KD.

VCAM1 Antibody – Rabbit Polyclonal

Name: VCAM1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750593
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750593

Description: CD106(cluster of differentiation 106) also known as vascular cell adhesion molecule 1(VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VCAM1/Cd106, Polyclonal Antibody

Full Product Name

Anti-VCAM1/Cd106 Picoband antibody

Product Gene Name

anti-VCAM1 antibody

Product Synonym Names

Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

74,346 Da

OMIM

M30257 mRNA

3D Structure

ModBase 3D Structure for P19320

Immunogen

E Coli-derived human VCAM1 recombinant protein (Position: F25-L270).

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-VCAM1 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-VCAM1 antibody

WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml

Other Notes

Small volumes of anti-VCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCAM1 purchase

MBS1750593 is an antibody targeting VCAM1. Also known as Anti-VCAM1/Cd106 Picoband antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.

NCBI GI #

4507875

NCBI GeneID

7412

NCBI Accession #

NP_001069.1

UniProt Primary Accession #

P19320

UniProt Secondary Accession #

Q6NUP8; A8K6R7; B4DKS4; E9PDD1

UniProt Related Accession #

P19320

GenBank Nucleotide #

NM_001078.3

NCBI Official Full Name

vascular cell adhesion protein 1 isoform a

NCBI Official Synonym Full Names

vascular cell adhesion molecule 1

NCBI Official Symbol

VCAM1

NCBI Official Synonym Symbols

CD106; INCAM-100

UniProt Protein Name

Vascular cell adhesion protein 1

UniProt Synonym Protein Names

INCAM-100; CD_antigen: CD106

Protein Family

Vascular cell adhesion protein

UniProt Gene Name

VCAM1

UniProt Synonym Gene Names

V-CAM 1; VCAM-1

NCBI Protein Information

vascular cell adhesion protein 1

NCBI Summary for VCAM1

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

UniProt Comments for VCAM1

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Research Articles on VCAM1

1. VCAM-1 knockdown reduced TNFalpha-induced HUVEC migration and tube formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VCAM1 antibody

Reference Product	PubMed Publications
CD44 antibody	>12 publications with VCAM1 and CD44
ITGA4 antibody	>7 publications with VCAM1 and ITGA4
IL13 antibody	>5 publications with VCAM1 and IL13
ITGB2 antibody	>5 publications with VCAM1 and ITGB2
ITGB1 antibody	>2 publications with VCAM1 and ITGB1
ITGAD antibody	>1 publications with VCAM1 and ITGAD
SRC antibody	>1 publications with VCAM1 and SRC

Pathways associated with anti-VCAM1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cellular Roles Of Anthrax Toxin Pathway antibodies	Cellular Roles Of Anthrax Toxin Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with anti-VCAM1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>58 publications with VCAM1 and Inflammation
Cardiovascular Diseases Antibodies	>43 publications with VCAM1 and Cardiovascular Diseases
Necrosis Antibodies	>41 publications with VCAM1 and Necrosis
Neoplasms Antibodies	>34 publications with VCAM1 and Neoplasms
Atherosclerosis Antibodies	>29 publications with VCAM1 and Atherosclerosis
Kidney Diseases Antibodies	>10 publications with VCAM1 and Kidney Diseases
Hypertension Antibodies	>10 publications with VCAM1 and Hypertension
Heart Diseases Antibodies	>9 publications with VCAM1 and Heart Diseases
Myocardial Ischemia Antibodies	>7 publications with VCAM1 and Myocardial Ischemia
Myocardial Infarction Antibodies	>6 publications with VCAM1 and Myocardial Infarction

Organs/Tissues associated with anti-VCAM1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>158 publications with VCAM1 and Vascular
Blood Antibodies	>89 publications with VCAM1 and Blood
Kidney Antibodies	>19 publications with VCAM1 and Kidney
Embryonic Tissue Antibodies	>19 publications with VCAM1 and Embryonic Tissue
Brain Antibodies	>14 publications with VCAM1 and Brain
Liver Antibodies	>11 publications with VCAM1 and Liver
Heart Antibodies	>10 publications with VCAM1 and Heart
Placenta Antibodies	>7 publications with VCAM1 and Placenta
Lung Antibodies	>7 publications with VCAM1 and Lung
Skin Antibodies	>6 publications with VCAM1 and Skin