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CDC45L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750628

Description: CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC45L, Polyclonal Antibody
Full Product Name
Anti-CDC45L Picoband antibody
Product Gene Name
anti-CDC45L antibody
Product Synonym Gene Name
CDC45
Product Synonym Names
Cell division control protein 45 homolog; PORC-PI-1; CDC45; CDC45L; CDC45L2; UNQ374/PRO710
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
68,768 Da
OMIM
AF062495 mRNA
3D Structure
ModBase 3D Structure for O75419
Immunogen
E Coli-derived human CDC45L recombinant protein (Position: E166-A431).
Subcellular Localization
Cytoplasm. Nucleus.
Tissue Specificity
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CDC45L antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CDC45L antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-CDC45L antibody

Figure 1. Western blot analysis of CDC45L using anti-CDC45L antibody (MBS1750628).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC45L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
Rabbit Anti-CDC45L Polyclonal Antibody – Cat# MBS1750628

Other Notes

Small volumes of anti-CDC45L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC45L purchase

MBS1750628 is an antibody targeting CDC45L. Also known as Anti-CDC45L Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC45L. It may not necessarily be applicable to this product.

NCBI GI #
295821205
NCBI GeneID
8318
NCBI Accession #
NP_001171481.1
UniProt Primary Accession #
O75419
UniProt Secondary Accession #
O60856; Q20WK8; Q6UW54; Q9UP68; B4DDB4; B4DDU3; E9PDH7
UniProt Related Accession #
O75419
GenBank Nucleotide #
NM_001178010.2
NCBI Official Full Name
cell division control protein 45 homolog isoform 1
NCBI Official Synonym Full Names
cell division cycle 45
NCBI Official Symbol
CDC45
NCBI Official Synonym Symbols
CDC45L; MGORS7; CDC45L2; PORC-PI-1
UniProt Protein Name
Cell division control protein 45 homolog
UniProt Synonym Protein Names
PORC-PI-1
UniProt Gene Name
CDC45
UniProt Synonym Gene Names
CDC45L; CDC45L2
NCBI Protein Information
cell division control protein 45 homolog

NCBI Summary for CDC45L

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for CDC45L

Required for initiation of chromosomal DNA replication.

Research Articles on CDC45L

1. strong evidence that Cdc45 directly loads RPA on the emerging nascent ssDNA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CDC45L antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-CDC45L antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with CDC45L and Cardiovascular Diseases
 Intestinal Neoplasms Antibodies  >2 publications with CDC45L and Intestinal Neoplasms
 Nervous System Diseases Antibodies  >2 publications with CDC45L and Nervous System Diseases
 Adenocarcinoma Antibodies  >2 publications with CDC45L and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with CDC45L and Carcinoma, Squamous Cell
 Inflammation Antibodies  >1 publications with CDC45L and Inflammation
 Nervous System Malformations Antibodies  >1 publications with CDC45L and Nervous System Malformations
 Precancerous Conditions Antibodies  >1 publications with CDC45L and Precancerous Conditions
 Breast Neoplasms Antibodies  >1 publications with CDC45L and Breast Neoplasms

Organs/Tissues associated with anti-CDC45L antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with CDC45L and Lung
 Embryonic Tissue Antibodies  >4 publications with CDC45L and Embryonic Tissue
 Blood Antibodies  >3 publications with CDC45L and Blood
 Heart Antibodies  >3 publications with CDC45L and Heart
 Brain Antibodies  >2 publications with CDC45L and Brain
 Ovary Antibodies  >1 publications with CDC45L and Ovary
 Bone Marrow Antibodies  >1 publications with CDC45L and Bone Marrow
 Placenta Antibodies  >1 publications with CDC45L and Placenta
 Thymus Antibodies  >1 publications with CDC45L and Thymus
 Lymph Antibodies  >1 publications with CDC45L and Lymph
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