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BNIP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750650

Description: The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain… More details

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Product Overview

Online Datasheet in PDF
Product Name
BNIP3, Polyclonal Antibody
Full Product Name
Anti-BNIP3 Picoband antibody
Product Gene Name
anti-BNIP3 antibody
Product Synonym Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3; NIP3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
27,832 Da
OMIM
AY886764 Genomic DNA
3D Structure
ModBase 3D Structure for Q12983
Immunogen
A synthetic peptide corresponding to a sequence of human BNIP3 (KEFLFKHPKRTATLSMRNTSVMKK).
Subcellular Localization
Mitochondrion. Mitochondrion outer membrane; Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BNIP3 antibody
Western Blot (WB)
Application Notes for anti-BNIP3 antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-BNIP3 antibody

Figure 1. Western blot analysis of BNIP3 using anti-BNIP3 antibody (MBS1750650).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BNIP3 at approximately 21KD. The expected band size for BNIP3 is at 21KD.
Rabbit Anti-BNIP3 Polyclonal Antibody – Cat# MBS1750650

Western Blot (WB) of anti-BNIP3 antibody

Figure 2. Western blot analysis of BNIP3 using anti-BNIP3 antibody (MBS1750650).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BNIP3 at approximately 21KD. The expected band size for BNIP3 is at 21KD.
Rabbit Anti-BNIP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1750650

Other Notes

Small volumes of anti-BNIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BNIP3 purchase

MBS1750650 is an antibody targeting BNIP3. Also known as Anti-BNIP3 Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BNIP3. It may not necessarily be applicable to this product.

NCBI GI #
734703979
NCBI GeneID
664
NCBI Accession #
NP_004043.3
UniProt Primary Accession #
Q12983
UniProt Secondary Accession #
O14620; Q96GP0
UniProt Related Accession #
Q12983
GenBank Nucleotide #
NM_004052.3
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
NCBI Official Synonym Full Names
BCL2 interacting protein 3
NCBI Official Symbol
BNIP3
NCBI Official Synonym Symbols
NIP3
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Protein Family
BCL2/adenovirus E1B 19 kDa protein-interacting protein
UniProt Gene Name
BNIP3
UniProt Synonym Gene Names
NIP3
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

NCBI Summary for BNIP3

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

UniProt Comments for BNIP3

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
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Research Articles on BNIP3

1. The authors find that KDM3A promotes anoikis through transcriptional activation of BNIP3 and BNIP3L, which encode pro-apoptotic proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BNIP3 antibody

 Reference Product  PubMed Publications
 HIF1A antibody  >102 publications with BNIP3 and HIF1A
 BNIP3L antibody  >94 publications with BNIP3 and BNIP3L
 BCL2 antibody  >85 publications with BNIP3 and BCL2
 TNF antibody  >20 publications with BNIP3 and TNF
 AKT1 antibody  >4 publications with BNIP3 and AKT1
 MAP1LC3B antibody  >3 publications with BNIP3 and MAP1LC3B
 GABARAP antibody  >3 publications with BNIP3 and GABARAP
 AIFM1 antibody  >3 publications with BNIP3 and AIFM1

Pathways associated with anti-BNIP3 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-BNIP3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >75 publications with BNIP3 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >62 publications with BNIP3 and Nervous System Diseases
 Necrosis Antibodies  >57 publications with BNIP3 and Necrosis
 Heart Diseases Antibodies  >43 publications with BNIP3 and Heart Diseases
 Brain Diseases Antibodies  >41 publications with BNIP3 and Brain Diseases
 Breast Neoplasms Antibodies  >33 publications with BNIP3 and Breast Neoplasms
 Disease Models, Animal Antibodies  >30 publications with BNIP3 and Disease Models, Animal
 Inflammation Antibodies  >18 publications with BNIP3 and Inflammation
 Neoplasms, Experimental Antibodies  >17 publications with BNIP3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >15 publications with BNIP3 and Kidney Diseases

Organs/Tissues associated with anti-BNIP3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >113 publications with BNIP3 and Muscle
 Heart Antibodies  >88 publications with BNIP3 and Heart
 Brain Antibodies  >64 publications with BNIP3 and Brain
 Vascular Antibodies  >61 publications with BNIP3 and Vascular
 Blood Antibodies  >61 publications with BNIP3 and Blood
 Lung Antibodies  >29 publications with BNIP3 and Lung
 Liver Antibodies  >29 publications with BNIP3 and Liver
 Bone Antibodies  >22 publications with BNIP3 and Bone
 Kidney Antibodies  >20 publications with BNIP3 and Kidney
 Prostate Antibodies  >10 publications with BNIP3 and Prostate
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