Rabbit Anti-TPX2 Polyclonal Antibody – Cat# MBS1750679

Figure 1. Western blot analysis of TPX2 using anti-TPX2 antibody (MBS1750679).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human SGC-7901 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TPX2 at approximately 86KD, 100KD. The expected band size for TPX2 is at 86KD.

TPX2 Antibody – Rabbit Polyclonal

Name: TPX2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750679
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750679

Description: Targeting protein for Xklp2, also known as C20ORF1, is a protein that in humans is encoded by the TPX2 gene. It is mapped to chromosome 20q11.2. It was concluded that even in the presence of duplicated centrosomes, spindle formation requires the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TPX2, Polyclonal Antibody

Full Product Name

Anti-TPX2 antibody

Product Gene Name

anti-TPX2 antibody

Product Synonym Names

Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

89,393 Da

OMIM

AB027467 mRNA

3D Structure

ModBase 3D Structure for Q9ULW0

Immunogen

E Coli-derived human TPX2 recombinant protein (Position: D15-Q93).

Subcellular Localization

Nucleus.

Tissue Specificity

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TPX2 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-TPX2 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-TPX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPX2 purchase

MBS1750679 is an antibody targeting TPX2. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TPX2. It may not necessarily be applicable to this product.

NCBI GI #

20127519

NCBI GeneID

22974

NCBI Accession #

NP_036244.2

UniProt Primary Accession #

Q9ULW0

UniProt Secondary Accession #

Q96RR5; Q9H1R4; Q9NRA3; Q9UFN9; Q9UL00; Q9Y2M1

UniProt Related Accession #

Q9ULW0

GenBank Nucleotide #

NM_012112.4

NCBI Official Full Name

targeting protein for Xklp2

NCBI Official Synonym Full Names

TPX2, microtubule nucleation factor

NCBI Official Symbol

TPX2

NCBI Official Synonym Symbols

DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1

UniProt Protein Name

Targeting protein for Xklp2

UniProt Synonym Protein Names

Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100

Protein Family

Protein

UniProt Gene Name

TPX2

UniProt Synonym Gene Names

C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100

NCBI Protein Information

targeting protein for Xklp2

UniProt Comments for TPX2

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).

Research Articles on TPX2

1. We show TPX2, a regulator of Aurora-A, is associated with high grade and stage of ccRCC, and is an independent predictor of recurrence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TPX2 antibody

Reference Product	PubMed Publications
AURKA antibody	>11 publications with TPX2 and AURKA
HMMR antibody	>2 publications with TPX2 and HMMR

Pathways associated with anti-TPX2 antibody

Products by Pathway	Pathway Diagram
Aurora A Signaling Pathway antibodies	Aurora A Signaling Pathway Diagram
Gastric Cancer Network 1 Pathway antibodies	Gastric Cancer Network 1 Pathway Diagram
PLK1 Signaling Events Pathway antibodies	PLK1 Signaling Events Pathway Diagram

Diseases associated with anti-TPX2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>40 publications with TPX2 and Neoplasms
Carcinoma, Squamous Cell Antibodies	>7 publications with TPX2 and Carcinoma, Squamous Cell
Lung Neoplasms Antibodies	>7 publications with TPX2 and Lung Neoplasms
Cell Transformation, Neoplastic Antibodies	>6 publications with TPX2 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>5 publications with TPX2 and Neoplasms, Experimental
Drug Toxicity Antibodies	>4 publications with TPX2 and Drug Toxicity
Breast Neoplasms Antibodies	>4 publications with TPX2 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>4 publications with TPX2 and Neoplasm Metastasis
Ovarian Diseases Antibodies	>4 publications with TPX2 and Ovarian Diseases
Ovarian Neoplasms Antibodies	>4 publications with TPX2 and Ovarian Neoplasms

Organs/Tissues associated with anti-TPX2 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>11 publications with TPX2 and Lung
Bone Antibodies	>4 publications with TPX2 and Bone
Brain Antibodies	>3 publications with TPX2 and Brain
Lymph Antibodies	>2 publications with TPX2 and Lymph
Prostate Antibodies	>2 publications with TPX2 and Prostate
Blood Antibodies	>2 publications with TPX2 and Blood
Bone Marrow Antibodies	>2 publications with TPX2 and Bone Marrow
Lymph Node Antibodies	>2 publications with TPX2 and Lymph Node
Kidney Antibodies	>1 publications with TPX2 and Kidney
Uterus Antibodies	>1 publications with TPX2 and Uterus