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Tropomyosin(sarcomeric) (TPM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175068

Description: Mouse IgG monoclonal antibody for Tropomyosin (Sarcomeric), tropomyosin 1 (alpha) (TPM1) detection. Tested with WB, IHC-P, IHC-F in Human, rat, chicken. No cross reactivity with other proteins. Uniprot ID: P09493Background: Tropomyosin is an… More details

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Product Overview

Product Name
Tropomyosin(Sarcomeric) (TPM1), Monoclonal Antibody
Full Product Name
Anti-Tropomyosin(Sarcomeric) antibody (monoclonal)
Product Gene Name
anti-TPM1 antibody
Product Synonym Names
Tropomyosin alpha-1 chain; tropomyosin 1(alpha); CMH3; TMSA; CMD1Y; C15orf13; HTM-alpha; Alpha-tropomyosin; Tropomyosin-1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ST-39
Host
Mouse
Species Reactivity
Human, Rat, Chicken
Form/Format
Lyophilized
Purity/Purification
Ascites
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
32,866 Da
OMIM
gene 611878
3D Structure
ModBase 3D Structure for P09493
Immunogen
Chicken muscle tropomyosin.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TPM1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Western Blot (WB) of anti-TPM1 antibody

Figure 1. Western blot analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human Caco-2 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tropomyosin(Sarcomeric) antigen affinity purified monoclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Tropomyosin(Sarcomeric) at approximately 39KD. The expected band size for Tropomyosin(Sarcomeric) is at 33KD.
Mouse Anti-TPM1 Monoclonal Antibody – Cat# MBS175068

Immunohistochemistry (IHC) of anti-TPM1 antibody

Figure 2. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Mouse Anti-TPM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175068

Immunohistochemistry (IHC) of anti-TPM1 antibody

Figure 3. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Mouse Anti-TPM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175068

Immunohistochemistry (IHC) of anti-TPM1 antibody

Figure 4. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Mouse Anti-TPM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175068

Immunohistochemistry (IHC) of anti-TPM1 antibody

Figure 5. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Mouse Anti-TPM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175068

Other Notes

Small volumes of anti-TPM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPM1 purchase

MBS175068 is an antibody targeting TPM1. Also known as Anti-Tropomyosin(Sarcomeric) antibody (monoclonal). Reactive in Human, Rat, Chicken. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPM1. It may not necessarily be applicable to this product.

NCBI GI #
136092
NCBI GeneID
7168
NCBI Accession #
P09493.2
UniProt Primary Accession #
P09493
UniProt Secondary Accession #
P09494; P10469; Q6DV89; Q6DV90; Q7Z6L8; Q86W64; Q96IK2; B7Z5T7; D9YZV2; D9YZV3; D9YZV8
UniProt Related Accession #
P09493
NCBI Official Full Name
Tropomyosin alpha-1 chain
NCBI Official Synonym Full Names
tropomyosin 1 (alpha)
NCBI Official Symbol
TPM1
NCBI Official Synonym Symbols
CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha
UniProt Protein Name
Tropomyosin alpha-1 chain
UniProt Synonym Protein Names
Alpha-tropomyosin; Tropomyosin-1
Protein Family
Tropomyosin
UniProt Gene Name
TPM1
UniProt Synonym Gene Names
C15orf13; TMSA
UniProt Entry Name
TPM1_HUMAN
NCBI Protein Information
tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3

NCBI Summary for TPM1

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
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UniProt Comments for TPM1

TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.

Protein type: Motor; Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.1

Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding

Biological Process: positive regulation of cell adhesion; wound healing; in utero embryonic development; regulation of muscle contraction; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3
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Research Articles on TPM1

1. Vardenafil administration improved erectile functionality in controlled type 2 diabetes mellitus patients with ED, which was associated with reduction of circulating plasma beta-tropomyosin levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPM1 antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-TPM1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >74 publications with TPM1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >73 publications with TPM1 and Heart Diseases
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >27 publications with TPM1 and Cardiomyopathy, Hypertrophic, Familial
 Hypertrophy Antibodies  >26 publications with TPM1 and Hypertrophy
 Breast Neoplasms Antibodies  >16 publications with TPM1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >12 publications with TPM1 and Cell Transformation, Neoplastic
 Fibrosis Antibodies  >10 publications with TPM1 and Fibrosis
 Disease Models, Animal Antibodies  >10 publications with TPM1 and Disease Models, Animal
 Cardiomyopathy, Familial Hypertrophic, 3 Antibodies  >9 publications with TPM1 and Cardiomyopathy, Familial Hypertrophic, 3
 Kidney Diseases Antibodies  >5 publications with TPM1 and Kidney Diseases

Organs/Tissues associated with anti-TPM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >103 publications with TPM1 and Muscle
 Heart Antibodies  >70 publications with TPM1 and Heart
 Blood Antibodies  >21 publications with TPM1 and Blood
 Vascular Antibodies  >15 publications with TPM1 and Vascular
 Embryonic Tissue Antibodies  >9 publications with TPM1 and Embryonic Tissue
 Lung Antibodies  >7 publications with TPM1 and Lung
 Kidney Antibodies  >5 publications with TPM1 and Kidney
 Brain Antibodies  >5 publications with TPM1 and Brain
 Bone Antibodies  >5 publications with TPM1 and Bone
 Liver Antibodies  >4 publications with TPM1 and Liver
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