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SCRIBBLE Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750687

Description: SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and… More details

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Product Overview

Online Datasheet in PDF
Product Name
SCRIBBLE, Polyclonal Antibody
Full Product Name
Anti-SCRIBBLE Picoband antibody
Product Gene Name
anti-SCRIBBLE antibody
Product Synonym Gene Name
SCRIB
Product Synonym Names
Protein scribble homolog; Scribble; hScrib; Protein LAP4; SCRIB; CRIB1; KIAA0147; LAP4; SCRB1; VARTUL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
177,694 Da
OMIM
AF240677 mRNA
3D Structure
ModBase 3D Structure for Q14160
Immunogen
E Coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
Subcellular Localization
Cell membrane.
Tissue Specificity
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SCRIBBLE antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-SCRIBBLE antibody
WB: 0.1-0.5mug/ml
Immunohistochemistry(Frozen Section): 0.5-1mug/ml
ICC: 0.5-1mug/ml
FC/FACS: 1-3g/1x10 6 cells
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-SCRIBBLE antibody

Figure 1. Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (MBS1750687).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human 22RV1 whole cell lysates,
Lane 4: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD.
Rabbit Anti-SCRIBBLE Polyclonal Antibody – Cat# MBS1750687

Flow Cytometry (FC/FACS) of anti-SCRIBBLE antibody

Figure 2. Flow Cytometry analysis of A549 cells using anti-SCR1B antibody (MBS1750687).
Overlay histogram showing A549 cells stained with MBS1750687 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCR1B Antibody (MBS1750687,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-SCRIBBLE Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1750687

Flow Cytometry (FC/FACS) of anti-SCRIBBLE antibody

Figure 3. Flow Cytometry analysis of HepG2 cells using anti-SCR1B antibody (MBS1750687).
Overlay histogram showing HepG2 cells stained with MBS1750687 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCR1B Antibody (MBS1750687,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-SCRIBBLE Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1750687

Other Notes

Small volumes of anti-SCRIBBLE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCRIBBLE purchase

MBS1750687 is an antibody targeting SCRIBBLE. Also known as Anti-SCRIBBLE Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SCRIBBLE. It may not necessarily be applicable to this product.

NCBI GI #
355390315
NCBI GeneID
23513
NCBI Accession #
NP_056171.3
UniProt Primary Accession #
Q14160
UniProt Secondary Accession #
Q6P496; Q7Z5D1; Q8WWV8; Q96C69; Q96GG1
UniProt Related Accession #
Q14160
GenBank Nucleotide #
NM_015356.4
NCBI Official Full Name
protein scribble homolog isoform b
NCBI Official Synonym Full Names
scribbled planar cell polarity protein
NCBI Official Symbol
SCRIB
NCBI Official Synonym Symbols
CRIB1; SCRB1; SCRIB1; Vartul
UniProt Protein Name
Protein scribble homolog
UniProt Synonym Protein Names
Protein LAP4
Protein Family
Protein scribble
UniProt Gene Name
SCRIB
UniProt Synonym Gene Names
CRIB1; KIAA0147; LAP4; SCRB1; VARTUL; Scribble; hScrib
NCBI Protein Information
protein scribble homolog

NCBI Summary for SCRIBBLE

This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for SCRIBBLE

Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
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Research Articles on SCRIBBLE

1. In this study the authors show that human Scrib expression is required for maintaining high levels of human papillomavirus type 18 E6 protein in HeLa cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SCRIBBLE antibody

 Reference Product  PubMed Publications
 ARHGEF7 antibody  >4 publications with SCRIBBLE and ARHGEF7
 GIT1 antibody  >3 publications with SCRIBBLE and GIT1
 CRTAM antibody  >1 publications with SCRIBBLE and CRTAM
 PHLPP1 antibody  >1 publications with SCRIBBLE and PHLPP1

Pathways associated with anti-SCRIBBLE antibody

 Products by Pathway  Pathway Diagram
 Asymmetric Localization Of PCP Proteins Pathway antibodies  Asymmetric Localization Of PCP Proteins Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 PCP/CE Pathway antibodies  PCP/CE Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TSH Signaling Pathway antibodies  TSH Signaling Pathway Diagram
 Viral Carcinogenesis Pathway antibodies  Viral Carcinogenesis Pathway Diagram
 Viral Carcinogenesis Pathway antibodies  Viral Carcinogenesis Pathway Diagram
 Beta-catenin Independent WNT Signaling Pathway antibodies  Beta-catenin Independent WNT Signaling Pathway Diagram

Diseases associated with anti-SCRIBBLE antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >16 publications with SCRIBBLE and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >12 publications with SCRIBBLE and Cell Transformation, Neoplastic
 Congenital Abnormalities Antibodies  >8 publications with SCRIBBLE and Congenital Abnormalities
 Nervous System Malformations Antibodies  >7 publications with SCRIBBLE and Nervous System Malformations
 Neoplasms, Experimental Antibodies  >6 publications with SCRIBBLE and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >4 publications with SCRIBBLE and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >4 publications with SCRIBBLE and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >4 publications with SCRIBBLE and Neoplasm Invasiveness
 Kidney Diseases Antibodies  >3 publications with SCRIBBLE and Kidney Diseases
 Lung Neoplasms Antibodies  >3 publications with SCRIBBLE and Lung Neoplasms

Organs/Tissues associated with anti-SCRIBBLE antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >14 publications with SCRIBBLE and Eye
 Kidney Antibodies  >14 publications with SCRIBBLE and Kidney
 Embryonic Tissue Antibodies  >9 publications with SCRIBBLE and Embryonic Tissue
 Nerve Antibodies  >9 publications with SCRIBBLE and Nerve
 Ovary Antibodies  >8 publications with SCRIBBLE and Ovary
 Brain Antibodies  >8 publications with SCRIBBLE and Brain
 Lung Antibodies  >7 publications with SCRIBBLE and Lung
 Liver Antibodies  >7 publications with SCRIBBLE and Liver
 Intestine Antibodies  >6 publications with SCRIBBLE and Intestine
 Blood Antibodies  >6 publications with SCRIBBLE and Blood
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