Rabbit Anti-DPYD Polyclonal Antibody – Cat# MBS1750709

Figure 1. Western blot analysis of DPYD using anti-DPYD antibody (MBS1750709).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DPYD at approximately 111KD. The expected band size for DPYD is at 111KD.

DPYD Antibody – Rabbit Polyclonal

Name: DPYD Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750709
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750709

Description: DPYD (Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DPYD, Polyclonal Antibody

Full Product Name

Anti-DPYD antibody

Product Gene Name

anti-DPYD antibody

Product Synonym Names

Dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPYD

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

18,936 Da

OMIM

U09178 mRNA

3D Structure

ModBase 3D Structure for Q12882

Immunogen

E Coli-derived human DPYD recombinant protein (Position: A356-Y511).

Subcellular Localization

Cytoplasm.

Tissue Specificity

Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-DPYD antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-DPYD antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-DPYD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPYD purchase

MBS1750709 is an antibody targeting DPYD. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DPYD. It may not necessarily be applicable to this product.

NCBI GI #

119943098

NCBI GeneID

1806

NCBI Accession #

NP_000101.2

UniProt Primary Accession #

Q12882

UniProt Secondary Accession #

Q16694; Q16761; Q32NB0; Q96HL6; Q96TH1; A2RRQ2; A2RRQ3; A8K5A2; A8MWG9; B1AN21; E9PFN1

UniProt Related Accession #

Q12882

GenBank Nucleotide #

NM_000110.3

NCBI Official Full Name

dihydropyrimidine dehydrogenase

NCBI Official Synonym Full Names

dihydropyrimidine dehydrogenase

NCBI Official Symbol

DPYD

NCBI Official Synonym Symbols

DHP; DPD; DHPDHASE

UniProt Protein Name

Dihydropyrimidine dehydrogenase [NADP(+)]

UniProt Synonym Protein Names

Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

Protein Family

Dihydropyrimidine dehydrogenase

UniProt Gene Name

DPYD

UniProt Synonym Gene Names

DHPDHase; DPD

NCBI Protein Information

dihydropyrimidine dehydrogenase [NADP(+)]

NCBI Summary for DPYD

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

UniProt Comments for DPYD

Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.

Research Articles on DPYD

1. Low DPYD expression is associated with colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DPYD antibody

Reference Product	PubMed Publications
TYMP antibody	>8 publications with DPYD and TYMP
CYP2A6 antibody	>3 publications with DPYD and CYP2A6
UPB1 antibody	>2 publications with DPYD and UPB1
DPYS antibody	>2 publications with DPYD and DPYS
UPP1 antibody	>1 publications with DPYD and UPP1
UMPS antibody	>1 publications with DPYD and UMPS

Pathways associated with anti-DPYD antibody

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram
Pantothenate And CoA Biosynthesis Pathway antibodies	Pantothenate And CoA Biosynthesis Pathway Diagram
Pyrimidine Catabolism Pathway antibodies	Pyrimidine Catabolism Pathway Diagram
Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies	Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram

Diseases associated with anti-DPYD antibody

Disease Name	Pubmed Publications
Dihydropyrimidine Dehydrogenase Deficiency Antibodies	>74 publications with DPYD and Dihydropyrimidine Dehydrogenase Deficiency
Gastrointestinal Diseases Antibodies	>66 publications with DPYD and Gastrointestinal Diseases
Gastrointestinal Neoplasms Antibodies	>65 publications with DPYD and Gastrointestinal Neoplasms
Colorectal Neoplasms Antibodies	>56 publications with DPYD and Colorectal Neoplasms
Carcinoma Antibodies	>36 publications with DPYD and Carcinoma
Purine-Pyrimidine Metabolism, Inborn Errors Antibodies	>36 publications with DPYD and Purine-Pyrimidine Metabolism, Inborn Errors
Breast Neoplasms Antibodies	>21 publications with DPYD and Breast Neoplasms
Diarrhea Antibodies	>21 publications with DPYD and Diarrhea
Adenocarcinoma Antibodies	>19 publications with DPYD and Adenocarcinoma
Neutropenia Antibodies	>18 publications with DPYD and Neutropenia

Organs/Tissues associated with anti-DPYD antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>65 publications with DPYD and Blood
Bone Antibodies	>30 publications with DPYD and Bone
Liver Antibodies	>16 publications with DPYD and Liver
Connective Tissue Antibodies	>10 publications with DPYD and Connective Tissue
Lung Antibodies	>8 publications with DPYD and Lung
Prostate Antibodies	>6 publications with DPYD and Prostate
Skin Antibodies	>5 publications with DPYD and Skin
Brain Antibodies	>5 publications with DPYD and Brain
Kidney Antibodies	>4 publications with DPYD and Kidney
Nerve Antibodies	>4 publications with DPYD and Nerve