CD2AP Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of CD2AP using anti-CD2AP antibody (MBS1750710).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human COLO-320 whole cell lysates,
Lane 5: human 22RV1 whole cell lysates,
Lane 6: human SK-OV-3 whole cell lysates,
Lane 7: rat stomach tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2AP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD2AP at approximately 85KD. The expected band size for CD2AP is at 71KD.

Figure 2. Flow Cytometry analysis of A431 cells using anti-CD2AP antibody (MBS1750710).
Overlay histogram showing A431 cells stained with MBS1750710 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD2AP Antibody (MBS1750710,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-CD2AP antibody (MBS1750710).
Overlay histogram showing CACO-2 cells stained with MBS1750710 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD2AP Antibody (MBS1750710,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 4. Flow Cytometry analysis of K562 cells using anti-CD2AP antibody (MBS1750710).
Overlay histogram showing K562 cells stained with MBS1750710 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD2AP Antibody (MBS1750710,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.