Rabbit Anti-KLK6 Polyclonal Antibody – Cat# MBS1750724

Figure 1. Western blot analysis of Kallikrein 6 using anti-Kallikrein 6 antibody (MBS1750724).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 cell lysates,
Lane 2: human COLO-320 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 6 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Kallikrein 6 at approximately 27KD. The expected band size for Kallikrein 6 is at 27KD.

Kallikrein 6 (KLK6) Antibody – Rabbit Polyclonal

Name: KLK6 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750724
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750724

Description: KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Kallikrein 6 (KLK6), Polyclonal Antibody

Full Product Name

Anti-Kallikrein 6 Picoband antibody

Product Gene Name

anti-KLK6 antibody

Product Synonym Names

Kallikrein-6; Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme; KLK6; PRSS18; PRSS9

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

4,333 Da

OMIM

U62801 mRNA

3D Structure

ModBase 3D Structure for Q92876

Immunogen

E Coli-derived human Kallikrein 6 recombinant protein (Position: L22-K244).

Subcellular Localization

Secreted. Nucleus, nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.

Tissue Specificity

In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-KLK6 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-KLK6 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-KLK6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLK6 purchase

MBS1750724 is an antibody targeting KLK6. Also known as Anti-Kallikrein 6 Picoband antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK6. It may not necessarily be applicable to this product.

NCBI GI #

61744424

NCBI GeneID

5653

NCBI Accession #

NP_001012982.1

UniProt Primary Accession #

Q92876

UniProt Secondary Accession #

Q6H301; A6NJA1; A8MW09

UniProt Related Accession #

Q92876

GenBank Nucleotide #

NM_001012964.2

NCBI Official Full Name

kallikrein-6 isoform A preproprotein

NCBI Official Synonym Full Names

kallikrein related peptidase 6

NCBI Official Symbol

KLK6

NCBI Official Synonym Symbols

hK6; Bssp; Klk7; SP59; PRSS9; PRSS18

UniProt Protein Name

Kallikrein-6

UniProt Synonym Protein Names

Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme

Protein Family

Kallikrein

UniProt Gene Name

KLK6

UniProt Synonym Gene Names

PRSS18; PRSS9

NCBI Protein Information

kallikrein-6

NCBI Summary for KLK6

This gene encodes a member of the kallikrein subfamily of the peptidase S1 family of serine proteases. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The encoded preproprotein is proteolytically processed to generate the mature protease. Expression of this protease is regulated by steroid hormones and may be elevated in multiple human cancers and in serum from psoriasis patients. The encoded protease may participate in the cleavage of amyloid precursor protein and alpha-synuclein, thus implicating this protease in Alzheimer's and Parkinson's disease, respectively. This gene is located in a gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

UniProt Comments for KLK6

Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.

Research Articles on KLK6

1. FST and KLK6 may have significance in breast cancer detection

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLK6 antibody

Reference Product	PubMed Publications
SPINK5 antibody	>3 publications with KLK6 and SPINK5
APP antibody	>1 publications with KLK6 and APP
SERPINC1 antibody	>1 publications with KLK6 and SERPINC1
SNCA antibody	>1 publications with KLK6 and SNCA

Pathways associated with anti-KLK6 antibody

Products by Pathway	Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram

Diseases associated with anti-KLK6 antibody

Disease Name	Pubmed Publications
Ovarian Neoplasms Antibodies	>41 publications with KLK6 and Ovarian Neoplasms
Carcinoma Antibodies	>40 publications with KLK6 and Carcinoma
Nervous System Diseases Antibodies	>33 publications with KLK6 and Nervous System Diseases
Breast Neoplasms Antibodies	>18 publications with KLK6 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>13 publications with KLK6 and Neoplasm Metastasis
Disease Models, Animal Antibodies	>13 publications with KLK6 and Disease Models, Animal
Inflammation Antibodies	>13 publications with KLK6 and Inflammation
Neoplasms, Experimental Antibodies	>8 publications with KLK6 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>6 publications with KLK6 and Lung Neoplasms
Lung Diseases Antibodies	>5 publications with KLK6 and Lung Diseases

Organs/Tissues associated with anti-KLK6 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>38 publications with KLK6 and Brain
Ascites Antibodies	>13 publications with KLK6 and Ascites
Spinal Cord Antibodies	>10 publications with KLK6 and Spinal Cord
Ovary Antibodies	>8 publications with KLK6 and Ovary
Lymph Node Antibodies	>8 publications with KLK6 and Lymph Node
Stomach Antibodies	>7 publications with KLK6 and Stomach
Lung Antibodies	>6 publications with KLK6 and Lung
Kidney Antibodies	>5 publications with KLK6 and Kidney
Mammary Gland Antibodies	>4 publications with KLK6 and Mammary Gland
Intestine Antibodies	>4 publications with KLK6 and Intestine