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PERK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750736

Description: Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates… More details

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Product Overview

Online Datasheet in PDF
Product Name
PERK, Polyclonal Antibody
Full Product Name
Anti-PERK Picoband antibody
Product Gene Name
anti-PERK antibody
Product Synonym Gene Name
EIF2AK3
Product Synonym Names
Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
125,216 Da
OMIM
AF110146 mRNA
3D Structure
ModBase 3D Structure for Q9NZJ5
Immunogen
E Coli-derived human PERK recombinant protein (Position: R222-Q334).
Subcellular Localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity
Ubiquitous. A high level expression is seen in secretory tissues.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PERK antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-PERK antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-PERK antibody

Figure 1. Western blot analysis of PERK using anti-PERK antibody (MBS1750736).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human SK-OV-3 whole cell lysates,
Lane 5: Human A431 whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PERK at approximately 140KD. The expected band size for PERK is at 125KD.
Rabbit Anti-PERK Polyclonal Antibody – Cat# MBS1750736

Other Notes

Small volumes of anti-PERK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PERK purchase

MBS1750736 is an antibody targeting PERK. Also known as Anti-PERK Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PERK. It may not necessarily be applicable to this product.

NCBI GI #
134304838
NCBI GeneID
9451
NCBI Accession #
NP_004827.4
UniProt Primary Accession #
Q9NZJ5
UniProt Secondary Accession #
O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9
UniProt Related Accession #
Q9NZJ5
GenBank Nucleotide #
NM_004836.6
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 3
NCBI Official Symbol
EIF2AK3
NCBI Official Synonym Symbols
PEK; WRS; PERK
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Synonym Protein Names
PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
Protein Family
Eukaryotic translation initiation factor 2-alpha kinase
UniProt Gene Name
EIF2AK3
UniProt Synonym Gene Names
PEK; PERK; HsPEK
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 3

NCBI Summary for PERK

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
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UniProt Comments for PERK

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
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Research Articles on PERK

1. miR-204 Targets PERK and Regulates UPR Signaling and beta-Cell Apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PERK antibody

 Reference Product  PubMed Publications
 HSPA5 antibody  >2 publications with PERK and HSPA5
 EIF2S1 antibody  >1 publications with PERK and EIF2S1
 DNAJC3 antibody  >1 publications with PERK and DNAJC3

Pathways associated with anti-PERK antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram

Diseases associated with anti-PERK antibody

 Disease Name  Pubmed Publications
 Wolcott-Rallison syndrome Antibodies  >28 publications with PERK and Wolcott-Rallison syndrome
 Nervous System Diseases Antibodies  >6 publications with PERK and Nervous System Diseases
 Liver Diseases Antibodies  >5 publications with PERK and Liver Diseases
 Brain Diseases Antibodies  >5 publications with PERK and Brain Diseases
 Supranuclear Palsy, Progressive Antibodies  >3 publications with PERK and Supranuclear Palsy, Progressive
 Drug Toxicity Antibodies  >2 publications with PERK and Drug Toxicity
 Renal Insufficiency Antibodies  >2 publications with PERK and Renal Insufficiency
 Hepatitis Antibodies  >2 publications with PERK and Hepatitis
 Atrophy Antibodies  >1 publications with PERK and Atrophy
 Cognition Disorders Antibodies  >1 publications with PERK and Cognition Disorders

Organs/Tissues associated with anti-PERK antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >16 publications with PERK and Pancreas
 Bone Antibodies  >13 publications with PERK and Bone
 Liver Antibodies  >11 publications with PERK and Liver
 Brain Antibodies  >8 publications with PERK and Brain
 Blood Antibodies  >5 publications with PERK and Blood
 Heart Antibodies  >2 publications with PERK and Heart
 Intestine Antibodies  >1 publications with PERK and Intestine
 Skin Antibodies  >1 publications with PERK and Skin
 Muscle Antibodies  >1 publications with PERK and Muscle
 Thyroid Antibodies  >1 publications with PERK and Thyroid
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