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Kallikrein 1 (KLK1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750789

Description: KLK1(KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Kallikrein 1 (KLK1), Polyclonal Antibody
Full Product Name
Anti-Kallikrein 1 antibody
Product Gene Name
anti-KLK1 antibody
Product Synonym Names
Kallikrein-1; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein; KLK1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
23,835 Da
OMIM
M25629 mRNA
3D Structure
ModBase 3D Structure for P06870
Immunogen
E Coli-derived human Kallikrein 1 recombinant protein (Position: I25-S262).
Tissue Specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KLK1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-KLK1 antibody
WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml

Western Blot (WB) of anti-KLK1 antibody

Figure 1. Western blot analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (MBS1750789).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human KLK1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Kallikrein 1 at approximately 32KD. The expected band size for Kallikrein 1 is at 29KD.
Rabbit Anti-KLK1 Polyclonal Antibody – Cat# MBS1750789

Other Notes

Small volumes of anti-KLK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLK1 purchase

MBS1750789 is an antibody targeting KLK1. Also known as Anti-Kallikrein 1 antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK1. It may not necessarily be applicable to this product.

NCBI GI #
4504875
NCBI GeneID
3816
NCBI Accession #
NP_002248.1
UniProt Primary Accession #
P06870
UniProt Secondary Accession #
Q66US9; Q86U61; Q8TCV8; Q9BS53; Q9NQU4; Q9UD19; Q9UMJ1
UniProt Related Accession #
P06870
GenBank Nucleotide #
NM_002257.3
NCBI Official Full Name
kallikrein-1 preproprotein
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
KLK1
NCBI Official Synonym Symbols
hK1; KLKR; Klk6
UniProt Protein Name
Kallikrein-1
UniProt Synonym Protein Names
Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein
Protein Family
Blarinasin
UniProt Gene Name
KLK1
NCBI Protein Information
kallikrein-1

NCBI Summary for KLK1

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]
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UniProt Comments for KLK1

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Research Articles on KLK1

1. Recognition of anti-tumor necrosis factor-alpha (TNF-alpha) or Kallikrein Inhibitor may lead to therapeutics to enhance existing treatments for patients who do not respond to anti-vascular endothelial growth factor (VEGF) therapies.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KLK1 antibody

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram

Diseases associated with anti-KLK1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >25 publications with KLK1 and Neoplasms
 Vascular Diseases Antibodies  >16 publications with KLK1 and Vascular Diseases
 Kidney Diseases Antibodies  >14 publications with KLK1 and Kidney Diseases
 Hypertension Antibodies  >13 publications with KLK1 and Hypertension
 Brain Diseases Antibodies  >8 publications with KLK1 and Brain Diseases
 Inflammation Antibodies  >7 publications with KLK1 and Inflammation
 Lung Diseases Antibodies  >6 publications with KLK1 and Lung Diseases
 Fibrosis Antibodies  >5 publications with KLK1 and Fibrosis
 Renal Insufficiency Antibodies  >3 publications with KLK1 and Renal Insufficiency
 Heart Diseases Antibodies  >3 publications with KLK1 and Heart Diseases

Organs/Tissues associated with anti-KLK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >23 publications with KLK1 and Kidney
 Skin Antibodies  >18 publications with KLK1 and Skin
 Heart Antibodies  >5 publications with KLK1 and Heart
 Salivary Gland Antibodies  >5 publications with KLK1 and Salivary Gland
 Pancreas Antibodies  >4 publications with KLK1 and Pancreas
 Ovary Antibodies  >2 publications with KLK1 and Ovary
 Thyroid Antibodies  >2 publications with KLK1 and Thyroid
 Bone Antibodies  >1 publications with KLK1 and Bone
 Uncharacterized Tissue Antibodies  >1 publications with KLK1 and Uncharacterized Tissue
 Intestine Antibodies  >1 publications with KLK1 and Intestine
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