Rabbit Anti-TNFRSF18 Polyclonal Antibody – Cat# MBS1750814

Figure 1. Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (MBS1750814).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 26KD. The expected band size for TNFRSF18 is at 26KD.

TNFRSF18 Antibody – Rabbit Polyclonal

Name: TNFRSF18 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750814
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750814

Description: Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TNFRSF18, Polyclonal Antibody

Full Product Name

Anti-TNFRSF18 Picoband antibody

Product Gene Name

anti-TNFRSF18 antibody

Product Synonym Names

Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD357; TNFRSF18; AITR; GITR; UNQ319/PRO364

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

25,110 Da

OMIM

AF125304 mRNA

3D Structure

ModBase 3D Structure for Q9Y5U5

Immunogen

E Coli-derived human TNFRSF18 recombinant protein (Position: Q26-P162).

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TNFRSF18 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-TNFRSF18 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-TNFRSF18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFRSF18 purchase

MBS1750814 is an antibody targeting TNFRSF18. Also known as Anti-TNFRSF18 Picoband antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFRSF18. It may not necessarily be applicable to this product.

NCBI GI #

4759246

NCBI GeneID

8784

NCBI Accession #

NP_004186.1

UniProt Primary Accession #

Q9Y5U5

UniProt Secondary Accession #

O95851; Q5U0I4; Q9NYJ9; B1AME1

UniProt Related Accession #

Q9Y5U5

GenBank Nucleotide #

NP_004186.1

NCBI Official Full Name

tumor necrosis factor receptor superfamily member 18 isoform 1

NCBI Official Synonym Full Names

TNF receptor superfamily member 18

NCBI Official Symbol

TNFRSF18

NCBI Official Synonym Symbols

AITR; GITR; CD357; GITR-D

UniProt Protein Name

Tumor necrosis factor receptor superfamily member 18

UniProt Synonym Protein Names

Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD_antigen: CD357

Protein Family

Tumor necrosis factor receptor superfamily

UniProt Gene Name

TNFRSF18

UniProt Synonym Gene Names

AITR; GITR

NCBI Protein Information

tumor necrosis factor receptor superfamily member 18

NCBI Summary for TNFRSF18

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

UniProt Comments for TNFRSF18

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Research Articles on TNFRSF18

1. HTLV-1 infection can modify the expression of main functional transcription factors, FOXP3 and GITR

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNFRSF18 antibody

Reference Product	PubMed Publications
FOXP3 antibody	>149 publications with TNFRSF18 and FOXP3
CTLA4 antibody	>71 publications with TNFRSF18 and CTLA4
TNFSF18 antibody	>55 publications with TNFRSF18 and TNFSF18
IL2RA antibody	>22 publications with TNFRSF18 and IL2RA
TRAF2 antibody	>3 publications with TNFRSF18 and TRAF2
TRAF1 antibody	>2 publications with TNFRSF18 and TRAF1
IL2RG antibody	>1 publications with TNFRSF18 and IL2RG

Pathways associated with anti-TNFRSF18 antibody

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
TNFR2 Non-canonical NF-kB Pathway antibodies	TNFR2 Non-canonical NF-kB Pathway Diagram
TNFs Bind Their Physiological Receptors Pathway antibodies	TNFs Bind Their Physiological Receptors Pathway Diagram

Diseases associated with anti-TNFRSF18 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>268 publications with TNFRSF18 and Necrosis
Inflammation Antibodies	>55 publications with TNFRSF18 and Inflammation
Disease Models, Animal Antibodies	>43 publications with TNFRSF18 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>16 publications with TNFRSF18 and Neoplasms, Experimental
Lung Diseases Antibodies	>16 publications with TNFRSF18 and Lung Diseases
Carcinoma Antibodies	>13 publications with TNFRSF18 and Carcinoma
Liver Diseases Antibodies	>10 publications with TNFRSF18 and Liver Diseases
Cardiovascular Diseases Antibodies	>8 publications with TNFRSF18 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>5 publications with TNFRSF18 and Breast Neoplasms
Edema Antibodies	>5 publications with TNFRSF18 and Edema

Organs/Tissues associated with anti-TNFRSF18 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>279 publications with TNFRSF18 and Blood
Lymph Node Antibodies	>33 publications with TNFRSF18 and Lymph Node
Lung Antibodies	>17 publications with TNFRSF18 and Lung
Intestine Antibodies	>7 publications with TNFRSF18 and Intestine
Brain Antibodies	>6 publications with TNFRSF18 and Brain
Kidney Antibodies	>5 publications with TNFRSF18 and Kidney