Rabbit Anti-THPO Polyclonal Antibody – Cat# MBS1750820

Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (MBS1750820).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,
Lane 2: rat spleen tissue lysate,
Lane 3: rat brain tissue lysate,
Lane 4: rat kidney tissue lysate,
Lane 5: rat thymus tissue lysate,
Lane 6: rat PC-12 cell lysate,
Lane 7: rat RH35 cell lysate,
Lane 8: mouse SP20 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.

Thrombopoietin (THPO) Antibody – Rabbit Polyclonal

Name: THPO Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750820
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750820

Description: Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Thrombopoietin (THPO), Polyclonal Antibody

Full Product Name

Anti-Thrombopoietin Picoband antibody

Product Gene Name

anti-THPO antibody

Product Synonym Names

Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

33,604 Da

OMIM

L33410 mRNA

3D Structure

ModBase 3D Structure for P40225

Immunogen

E Coli-derived rat Thrombopoietin recombinant protein (Position: A27-H259).

Subcellular Localization

Secreted.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-THPO antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-THPO antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-THPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THPO purchase

MBS1750820 is an antibody targeting THPO. Also known as Anti-Thrombopoietin Picoband antibody. Reactive in Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for THPO. It may not necessarily be applicable to this product.

NCBI GI #

4507493

NCBI GeneID

7066

NCBI Accession #

NP_000451.1

UniProt Primary Accession #

P40225

UniProt Secondary Accession #

Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8

UniProt Related Accession #

P40225

GenBank Nucleotide #

NM_000460.3

NCBI Official Full Name

thrombopoietin isoform 1

NCBI Official Synonym Full Names

thrombopoietin

NCBI Official Symbol

THPO

NCBI Official Synonym Symbols

ML; TPO; MGDF; MKCSF; MPLLG; THCYT1

UniProt Protein Name

Thrombopoietin

UniProt Synonym Protein Names

C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

Protein Family

Thrombopoietin

UniProt Gene Name

THPO

UniProt Synonym Gene Names

MGDF; ML; MGDF

NCBI Protein Information

thrombopoietin

NCBI Summary for THPO

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

UniProt Comments for THPO

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Research Articles on THPO

1. These studies demonstrate that biallelic loss-of-function mutations in THPO cause bone marrow failure, which is unresponsive to transplant due to a hematopoietic cell-extrinsic mechanism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-THPO antibody

Reference Product	PubMed Publications
MPL antibody	>34 publications with THPO and MPL
JAK2 antibody	>4 publications with THPO and JAK2
STAT3 antibody	>1 publications with THPO and STAT3

Pathways associated with anti-THPO antibody

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Platelet Aggregation (Plug Formation) Pathway antibodies	Platelet Aggregation (Plug Formation) Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with anti-THPO antibody

Disease Name	Pubmed Publications
Thrombocytosis Antibodies	>11 publications with THPO and Thrombocytosis
Anemia Antibodies	>8 publications with THPO and Anemia
Hemorrhage Antibodies	>3 publications with THPO and Hemorrhage
Lung Neoplasms Antibodies	>3 publications with THPO and Lung Neoplasms
Disease Models, Animal Antibodies	>3 publications with THPO and Disease Models, Animal
Neurobehavioral Manifestations Antibodies	>2 publications with THPO and Neurobehavioral Manifestations
Growth Disorders Antibodies	>2 publications with THPO and Growth Disorders
Thrombocythemia, Essential Antibodies	>2 publications with THPO and Thrombocythemia, Essential
Precancerous Conditions Antibodies	>1 publications with THPO and Precancerous Conditions
Prenatal Injuries Antibodies	>1 publications with THPO and Prenatal Injuries

Organs/Tissues associated with anti-THPO antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>7 publications with THPO and Liver
Connective Tissue Antibodies	>1 publications with THPO and Connective Tissue