Rabbit Anti-PDK2 Polyclonal Antibody – Cat# MBS1750824

Figure 1. Western blot analysis of PDK2 using anti-PDK2 antibody (MBS1750824).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDK2 at approximately 46KD. The expected band size for PDK2 is at 46KD.

PDK2 Antibody – Rabbit Polyclonal

Name: PDK2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750824
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750824

Description: PDK2 (Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDK2, Polyclonal Antibody

Full Product Name

Anti-PDK2 Picoband antibody

Product Gene Name

anti-PDK2 antibody

Product Synonym Names

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII; PDK2; PDHK2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

38,864 Da

OMIM

L42451 mRNA

3D Structure

ModBase 3D Structure for Q15119

Immunogen

E Coli-derived human PDK2 recombinant protein (Position: V81-D177).

Subcellular Localization

Mitochondrion matrix.

Tissue Specificity

Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-PDK2 antibody

Signal Transduction; Metabolism; Energy Metabolism; Signal Transduction; Mitochondrial; Cancer; Cancer Metabolism; Metabolic Signaling Pathway; Integration of Energy Metabolism; Metabolism; Pathways and Processes; Mitochondrial Metabolism; Mitochondrial M

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PDK2 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-PDK2 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-PDK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDK2 purchase

MBS1750824 is an antibody targeting PDK2. Also known as Anti-PDK2 Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDK2. It may not necessarily be applicable to this product.

NCBI GI #

315630349

NCBI GeneID

5164

NCBI Accession #

NP_001186827.1

UniProt Primary Accession #

Q15119

UniProt Secondary Accession #

Q6P515; Q9BS05; A8K3A7; B3KNW0

UniProt Related Accession #

Q15119

GenBank Nucleotide #

NM_001199898.1

NCBI Official Full Name

pyruvate dehydrogenase kinase, isozyme 2 isoform 2

NCBI Official Synonym Full Names

pyruvate dehydrogenase kinase 2

NCBI Official Symbol

PDK2

NCBI Official Synonym Symbols

PDHK2; PDKII

UniProt Protein Name

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial

UniProt Synonym Protein Names

Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII

UniProt Gene Name

PDK2

UniProt Synonym Gene Names

PDHK2; PDH kinase 2; PDKII

NCBI Protein Information

pyruvate dehydrogenase kinase, isozyme 2

NCBI Summary for PDK2

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

UniProt Comments for PDK2

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.

Research Articles on PDK2

1. PDK2/PARL senses defects in mitochondrial bioenergetics.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDK2 antibody

Reference Product	PubMed Publications
PDK1 antibody	>51 publications with PDK2 and PDK1
AKT1 antibody	>10 publications with PDK2 and AKT1
SGK1 antibody	>2 publications with PDK2 and SGK1

Pathways associated with anti-PDK2 antibody

Products by Pathway	Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram
Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway antibodies	Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway Diagram
Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway antibodies	Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway Diagram
The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies	The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram

Diseases associated with anti-PDK2 antibody

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>8 publications with PDK2 and Fatty Liver
Nervous System Diseases Antibodies	>5 publications with PDK2 and Nervous System Diseases
Liver Diseases Antibodies	>4 publications with PDK2 and Liver Diseases
Cardiovascular Diseases Antibodies	>4 publications with PDK2 and Cardiovascular Diseases
Drug Toxicity Antibodies	>3 publications with PDK2 and Drug Toxicity
Hyperglycemia Antibodies	>3 publications with PDK2 and Hyperglycemia
Kidney Diseases Antibodies	>3 publications with PDK2 and Kidney Diseases
Breast Neoplasms Antibodies	>2 publications with PDK2 and Breast Neoplasms
Inflammation Antibodies	>2 publications with PDK2 and Inflammation
Neoplasms, Experimental Antibodies	>2 publications with PDK2 and Neoplasms, Experimental

Organs/Tissues associated with anti-PDK2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>29 publications with PDK2 and Muscle
Liver Antibodies	>16 publications with PDK2 and Liver
Blood Antibodies	>14 publications with PDK2 and Blood
Heart Antibodies	>14 publications with PDK2 and Heart
Kidney Antibodies	>9 publications with PDK2 and Kidney
Pancreas Antibodies	>7 publications with PDK2 and Pancreas
Brain Antibodies	>6 publications with PDK2 and Brain
Adipose Tissue Antibodies	>4 publications with PDK2 and Adipose Tissue
Testis Antibodies	>3 publications with PDK2 and Testis
Lung Antibodies	>3 publications with PDK2 and Lung