Rabbit Anti-VEGFB Polyclonal Antibody – Cat# MBS1750875

Figure 1. Western blot analysis of VEGFB using anti-VEGFB antibody (MBS1750875).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human 22RV1 whole cell lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.

VEGFB Antibody – Rabbit Polyclonal

Name: VEGFB Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750875
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750875

Description: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VEGFB, Polyclonal Antibody

Full Product Name

Anti-VEGFB Picoband antibody

Product Gene Name

anti-VEGFB antibody

Product Synonym Names

Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; VEGFB; VRF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

21,261 Da

OMIM

U43368 mRNA

3D Structure

ModBase 3D Structure for P49765

Immunogen

E Coli-derived human VEGFB recombinant protein (Position: P22-A207).

Subcellular Localization

Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Tissue Specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-VEGFB antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-VEGFB antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-VEGFB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VEGFB purchase

MBS1750875 is an antibody targeting VEGFB. Also known as Anti-VEGFB Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VEGFB. It may not necessarily be applicable to this product.

NCBI GI #

344179103

NCBI GeneID

7423

NCBI Accession #

NP_001230662.1

UniProt Primary Accession #

P49765

UniProt Secondary Accession #

Q16528

UniProt Related Accession #

P49765

GenBank Nucleotide #

NM_001243733.1

NCBI Official Full Name

vascular endothelial growth factor B isoform VEGFB-167

NCBI Official Synonym Full Names

vascular endothelial growth factor B

NCBI Official Symbol

VEGFB

NCBI Official Synonym Symbols

VRF; VEGFL

UniProt Protein Name

Vascular endothelial growth factor B

UniProt Synonym Protein Names

VEGF-related factor; VRF

Protein Family

Vascular endothelial growth factor

UniProt Gene Name

VEGFB

UniProt Synonym Gene Names

VRF; VEGF-B; VRF

NCBI Protein Information

vascular endothelial growth factor B

NCBI Summary for VEGFB

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

UniProt Comments for VEGFB

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Research Articles on VEGFB

1. Cardiac transgenic vascular endothelial growth factor-B overexpression failed to protect heart transplants from ischemia-reperfusion injury.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VEGFB antibody

Reference Product	PubMed Publications
VEGFA antibody	>38 publications with VEGFB and VEGFA
FLT1 antibody	>8 publications with VEGFB and FLT1
NRP1 antibody	>2 publications with VEGFB and NRP1

Pathways associated with anti-VEGFB antibody

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram

Diseases associated with anti-VEGFB antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>32 publications with VEGFB and Neoplasms
Heart Diseases Antibodies	>11 publications with VEGFB and Heart Diseases
Nervous System Diseases Antibodies	>7 publications with VEGFB and Nervous System Diseases
Hypertrophy Antibodies	>6 publications with VEGFB and Hypertrophy
Edema Antibodies	>5 publications with VEGFB and Edema
Liver Neoplasms Antibodies	>5 publications with VEGFB and Liver Neoplasms
Liver Diseases Antibodies	>5 publications with VEGFB and Liver Diseases
Drug Toxicity Antibodies	>5 publications with VEGFB and Drug Toxicity
Hypertension Antibodies	>5 publications with VEGFB and Hypertension
Myocardial Ischemia Antibodies	>5 publications with VEGFB and Myocardial Ischemia

Organs/Tissues associated with anti-VEGFB antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>74 publications with VEGFB and Vascular
Blood Antibodies	>34 publications with VEGFB and Blood
Heart Antibodies	>15 publications with VEGFB and Heart
Muscle Antibodies	>13 publications with VEGFB and Muscle
Brain Antibodies	>9 publications with VEGFB and Brain
Liver Antibodies	>7 publications with VEGFB and Liver
Lung Antibodies	>7 publications with VEGFB and Lung
Placenta Antibodies	>6 publications with VEGFB and Placenta
Kidney Antibodies	>5 publications with VEGFB and Kidney
Connective Tissue Antibodies	>5 publications with VEGFB and Connective Tissue