Figure 4. IHC analysis of COPE using anti-COPE antibody (MBS1750876).
COPE was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COPE Antibody (MBS1750876) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 2. IHC analysis of COPE using anti-COPE antibody (MBS1750876).
COPE was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COPE Antibody (MBS1750876) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 3. IHC analysis of COPE using anti-COPE antibody (MBS1750876).
COPE was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COPE Antibody (MBS1750876) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Figure 1. Western blot analysis of COPE using anti-COPE antibody (MBS1750876).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysate,
Lane 2: rat small intestine tissue lysate,
Lane 3: rat pancreas tissue lysate,
Lane 4: mouse stomach tissue lysate,
Lane 5: mouse small intestine tissue lysate,
Lane 6: mouse pancreas tissue lysate,
Lane 7: human MCF-7 whole Cell lysatem,
Lane 8: human Hela whole Cell lysate,
Lane 9: human 22RV1 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
ELISA Kit
CLIA Kit
Assay Kit
Antibodies
Polyclonal Antibody
Secondary Antibody
Antigen
Biochemical
cDNA Clone
siRNA
Peptide
Rec./Purified Protein
Custom ELISA Kit
Custom Protein
Custom Antibody
Antibody Matched Pairs
Antibody Peptide Pairs
Phospho Antibodies
Products by Disease
Products by Pathway
Products by Tissue