Paxillin (PXN) Antibody – Mouse Monoclonal

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Adaptor/scaffold; Cytoskeletal; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; cell cortex; cytosol

Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: lamellipodium biogenesis; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; focal adhesion formation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; cell adhesion; vascular endothelial growth factor receptor signaling pathway