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CHX10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750894

Description: Visual system homeobox 2 is a protein that in humans is encoded by the VSX2 gene. This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia,… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHX10, Polyclonal Antibody
Full Product Name
Anti-CHX10 Picoband Antibody
Product Gene Name
anti-CHX10 antibody
Product Synonym Gene Name
VSX2
Product Synonym Names
Visual system homeobox 2; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; VSX2; CHX10; HOX10
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
39411 MW
OMIM
AY336059 mRNA
3D Structure
ModBase 3D Structure for P58304
Immunogen
E Coli-derived human CHX10 recombinant protein (Position: A249-A361). Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10.
Subcellular Localization
Nucleus.
Tissue Specificity
Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-CHX10 antibody

Neuroscience; Sensory System; Visual System; Neuroscience; Neurology Process; Neurogenesis; Epigenetics and Nuclear Signaling; ChIP'ing Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CHX10 antibody
Western Blot (WB)
Application Notes for anti-CHX10 antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-CHX10 antibody

Figure 1. Western blot analysis of CHX10 using anti-CHX10 antibody (MBS1750894).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse eye ball tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHX10 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHX10 at approximately 43KD. The expected band size for CHX10 is at 39KD.
Rabbit Anti-CHX10 Polyclonal Antibody – Cat# MBS1750894

Other Notes

Small volumes of anti-CHX10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHX10 purchase

MBS1750894 is an antibody targeting CHX10. Also known as Anti-CHX10 Picoband Antibody. Reactive in Human, Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHX10. It may not necessarily be applicable to this product.

NCBI GI #
34365783
NCBI GeneID
338917
NCBI Accession #
NP_878314.1
UniProt Primary Accession #
P58304
UniProt Secondary Accession #
A1A4X6
UniProt Related Accession #
P58304
GenBank Nucleotide #
NM_182894.2
NCBI Official Full Name
visual system homeobox 2
NCBI Official Synonym Full Names
visual system homeobox 2
NCBI Official Symbol
VSX2
NCBI Official Synonym Symbols
RET1; CHX10; HOX10; MCOP2; MCOPCB3
UniProt Protein Name
Visual system homeobox 2
UniProt Synonym Protein Names
Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10
Protein Family
Visual system homeobox
UniProt Gene Name
VSX2
UniProt Synonym Gene Names
CHX10; HOX10
NCBI Protein Information
visual system homeobox 2

NCBI Summary for CHX10

This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities. [provided by RefSeq, Oct 2009]

UniProt Comments for CHX10

Plays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells ().

Research Articles on CHX10

1. Regulation of WNT signaling by VSX2 during optic vesicle patterning in human induced pluripotent stem cells has been described.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHX10 antibody

 Reference Product  PubMed Publications
 PAX6 antibody  >24 publications with CHX10 and PAX6
 NES antibody  >6 publications with CHX10 and NES
 NEUROD4 antibody  >3 publications with CHX10 and NEUROD4
 SIX3 antibody  >3 publications with CHX10 and SIX3
 POU4F2 antibody  >3 publications with CHX10 and POU4F2
 CDKN1B antibody  >3 publications with CHX10 and CDKN1B
 GDF3 antibody  >1 publications with CHX10 and GDF3
 MFRP antibody  >1 publications with CHX10 and MFRP
 NXNL1 antibody  >1 publications with CHX10 and NXNL1

Diseases associated with anti-CHX10 antibody

 Disease Name  Pubmed Publications
 Embryo Loss Antibodies  >8 publications with CHX10 and Embryo Loss
 Disease Models, Animal Antibodies  >6 publications with CHX10 and Disease Models, Animal
 Microphthalmia, Isolated 2 Antibodies  >4 publications with CHX10 and Microphthalmia, Isolated 2
 Premature Birth Antibodies  >1 publications with CHX10 and Premature Birth
 Uterine Diseases Antibodies  >1 publications with CHX10 and Uterine Diseases
 Atrophy Antibodies  >1 publications with CHX10 and Atrophy
 Uterine Neoplasms Antibodies  >1 publications with CHX10 and Uterine Neoplasms
 Weight Loss Antibodies  >1 publications with CHX10 and Weight Loss

Organs/Tissues associated with anti-CHX10 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >89 publications with CHX10 and Eye
 Brain Antibodies  >10 publications with CHX10 and Brain
 Muscle Antibodies  >3 publications with CHX10 and Muscle
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