Rabbit Anti-RND1 Polyclonal Antibody – Cat# MBS1750909

Figure 1. Western blot analysis of RND1 using anti-RND1 antibody (MBS1750909).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RND1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RND1 at approximately 26KD. The expected band size for RND1 is at 26KD.

RND1 Antibody – Rabbit Polyclonal

Name: RND1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750909
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750909

Description: Rnd1 is a small (~21 kDa) signaling G protein (to be specific, a GTPase), and is a member of the Rndsubgroup of the Rho family of GTPases. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RND1, Polyclonal Antibody

Full Product Name

Anti-RND1 Picoband Antibody

Product Gene Name

anti-RND1 antibody

Product Synonym Names

Rho-related GTP-binding protein Rho6; Rho family GTPase 1; Rnd1; RND1; RHO6

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

26056 MW

OMIM

Y07923 mRNA

3D Structure

ModBase 3D Structure for Q92730

Immunogen

E Coli-derived human RND1 recombinant protein (Position: K105-C229). Human RND1 shares 95.2% amino acid (aa) sequence identity with mouse RND1.

Subcellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton.

Tissue Specificity

Mostly expressed in brain and liver.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-RND1 antibody

Signal Transduction; Cytoskeleton/ECM; Cytoskeleton; microfilaments; actin etc; actin assembly; Signal Transduction; Signaling Pathway; G Protein Signaling; Small G Proteins; ras family

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-RND1 antibody

Western Blot (WB)

Application Notes for anti-RND1 antibody

WB: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-RND1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RND1 purchase

MBS1750909 is an antibody targeting RND1. Also known as Anti-RND1 Picoband Antibody. Reactive in Human, Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RND1. It may not necessarily be applicable to this product.

NCBI GI #

7657514

NCBI GeneID

27289

NCBI Accession #

NP_055285.1

UniProt Primary Accession #

Q92730

UniProt Secondary Accession #

A8K9P7

UniProt Related Accession #

Q92730

GenBank Nucleotide #

NM_014470.3

NCBI Official Full Name

rho-related GTP-binding protein Rho6

NCBI Official Synonym Full Names

Rho family GTPase 1

NCBI Official Symbol

RND1

NCBI Official Synonym Symbols

ARHS; RHO6; RHOS

UniProt Protein Name

Rho-related GTP-binding protein Rho6

UniProt Synonym Protein Names

Rho family GTPase 1; Rnd1

Protein Family

Rho-related GTP-binding protein

UniProt Gene Name

RND1

UniProt Synonym Gene Names

RHO6

NCBI Protein Information

rho-related GTP-binding protein Rho6

NCBI Summary for RND1

This gene encodes a protein that belongs to the Rho GTPase family. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. A similar protein in rat interacts with a microtubule regulator to control axon extension. [provided by RefSeq, Apr 2014]

UniProt Comments for RND1

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments.

Research Articles on RND1

1. Analysis of the interaction of Plexin-B1 and Plexin-B2 with Rnd family proteins shows lack of binding specificity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RND1 antibody

Reference Product	PubMed Publications
PLXNB1 antibody	>6 publications with RND1 and PLXNB1
SEMA4D antibody	>4 publications with RND1 and SEMA4D
SEMA3A antibody	>3 publications with RND1 and SEMA3A
PLXNA1 antibody	>2 publications with RND1 and PLXNA1
ROCK2 antibody	>1 publications with RND1 and ROCK2
GRLF1 antibody	>1 publications with RND1 and GRLF1
FRS2 antibody	>1 publications with RND1 and FRS2
GRB7 antibody	>1 publications with RND1 and GRB7

Pathways associated with anti-RND1 antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway antibodies	SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway Diagram
Sema4D In Semaphorin Signaling Pathway antibodies	Sema4D In Semaphorin Signaling Pathway Diagram
Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies	Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway antibodies	Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with anti-RND1 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>5 publications with RND1 and Necrosis
Nervous System Diseases Antibodies	>3 publications with RND1 and Nervous System Diseases
Inflammation Antibodies	>3 publications with RND1 and Inflammation
Pain Antibodies	>1 publications with RND1 and Pain
Abnormalities, Multiple Antibodies	>1 publications with RND1 and Abnormalities, Multiple
Disease Models, Animal Antibodies	>1 publications with RND1 and Disease Models, Animal
Drug-Related Side Effects and Adverse Reactions Antibodies	>1 publications with RND1 and Drug-Related Side Effects and Adverse Reactions
Neoplasms, Experimental Antibodies	>1 publications with RND1 and Neoplasms, Experimental
Heart Diseases Antibodies	>1 publications with RND1 and Heart Diseases
Kidney Diseases Antibodies	>1 publications with RND1 and Kidney Diseases

Organs/Tissues associated with anti-RND1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with RND1 and Brain
Embryonic Tissue Antibodies	>4 publications with RND1 and Embryonic Tissue
Bone Antibodies	>3 publications with RND1 and Bone
Testis Antibodies	>2 publications with RND1 and Testis
Lung Antibodies	>2 publications with RND1 and Lung
Heart Antibodies	>1 publications with RND1 and Heart
Liver Antibodies	>1 publications with RND1 and Liver