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MAD Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750912

Description: MAD protein is a protein that in humans is encoded by the MXD1 gene. This gene encodes a member of the MYC/MAX/MAD network of basic helix-loop-helix leucine zipper transcription factors. The MYC/MAX/MAD transcription factors mediate cellular… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mad, Polyclonal Antibody
Full Product Name
Anti-Mad Picoband antibody
Product Gene Name
anti-Mad antibody
Product Synonym Gene Name
MXD1
Product Synonym Names
Max dimerization protein 1; Max dimerizer 1; Protein MAD; MXD1; MAD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
24,027 Da
OMIM
L06895 mRNA
3D Structure
ModBase 3D Structure for Q05195
Immunogen
E Coli-derived human Mad recombinant protein (Position: M1-L221).
Subcellular Localization
Nucleus.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Mad antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-Mad antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Western Blot (WB) of anti-Mad antibody

Figure 1. Western blot analysis of Mad using anti-Mad antibody (MBS1750912).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse ovary tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mad antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mad at approximately 28KD. The expected band size for Mad is at 25KD.
Rabbit Anti-MAD Polyclonal Antibody – Cat# MBS1750912

Other Notes

Small volumes of anti-Mad antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mad purchase

MBS1750912 is an antibody targeting Mad. Also known as Anti-Mad Picoband antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Mad. It may not necessarily be applicable to this product.

NCBI GI #
321400051
NCBI GeneID
4084
NCBI Accession #
NP_001189442.1
UniProt Primary Accession #
Q05195
UniProt Secondary Accession #
Q6FI41; B2R6V8; B7ZLI6; D6W5G2
UniProt Related Accession #
Q05195
GenBank Nucleotide #
NM_001202513.1
NCBI Official Full Name
max dimerization protein 1 isoform 2
NCBI Official Synonym Full Names
MAX dimerization protein 1
NCBI Official Symbol
MXD1
NCBI Official Synonym Symbols
MAD; MAD1; BHLHC58
UniProt Protein Name
Max dimerization protein 1
UniProt Synonym Protein Names
Protein MAD
Protein Family
Protein mothers against dpp
UniProt Gene Name
MXD1
UniProt Synonym Gene Names
MAD; Max dimerizer 1
NCBI Protein Information
max dimerization protein 1

NCBI Summary for Mad

This gene encodes a member of the MYC/MAX/MAD network of basic helix-loop-helix leucine zipper transcription factors. The MYC/MAX/MAD transcription factors mediate cellular proliferation, differentiation and apoptosis. The encoded protein antagonizes MYC-mediated transcriptional activation of target genes by competing for the binding partner MAX and recruiting repressor complexes containing histone deacetylases. Mutations in this gene may play a role in acute leukemia, and the encoded protein is a potential tumor suppressor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]
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UniProt Comments for Mad

Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX.

Research Articles on Mad

1. Study revealed that HIF-1alpha-induced Mxd1 contributes to cisplatin-resistance in hypoxic osteosarcoma cells by directly repressing PTEN, which leads to the activation of PI3K/AKT antiapoptotic and survival pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mad antibody

 Reference Product  PubMed Publications
 MAX antibody  >70 publications with Mad and MAX
 SIN3A antibody  >4 publications with Mad and SIN3A
 HDAC1 antibody  >3 publications with Mad and HDAC1
 HDAC2 antibody  >2 publications with Mad and HDAC2

Pathways associated with anti-Mad antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with anti-Mad antibody

 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >6 publications with Mad and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >4 publications with Mad and Nervous System Diseases
 Chromosome Aberrations Antibodies  >3 publications with Mad and Chromosome Aberrations
 Atherosclerosis Antibodies  >2 publications with Mad and Atherosclerosis
 Ovarian Neoplasms Antibodies  >2 publications with Mad and Ovarian Neoplasms
 Liver Neoplasms Antibodies  >2 publications with Mad and Liver Neoplasms
 Lung Neoplasms Antibodies  >2 publications with Mad and Lung Neoplasms
 Breast Neoplasms Antibodies  >2 publications with Mad and Breast Neoplasms
 Liver Diseases Antibodies  >2 publications with Mad and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with Mad and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-Mad antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with Mad and Blood
 Skin Antibodies  >5 publications with Mad and Skin
 Lung Antibodies  >4 publications with Mad and Lung
 Brain Antibodies  >3 publications with Mad and Brain
 Testis Antibodies  >2 publications with Mad and Testis
 Intestine Antibodies  >2 publications with Mad and Intestine
 Pancreas Antibodies  >2 publications with Mad and Pancreas
 Liver Antibodies  >2 publications with Mad and Liver
 Placenta Antibodies  >2 publications with Mad and Placenta
 Heart Antibodies  >2 publications with Mad and Heart
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