Rabbit Anti-MED20 Polyclonal Antibody – Cat# MBS1750937

Figure 1. Western blot analysis of TRFP / MED20 using anti-TRFP / MED20 antibody (MBS1750937).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRFP / MED20 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRFP / MED20 at approximately 23KD. The expected band size for TRFP / MED20 is at 23KD.

TRFP/MED20 (MED20) Antibody – Rabbit Polyclonal

Name: MED20 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1750937
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750937

Description: MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TRFP/MED20, Polyclonal Antibody

Full Product Name

Anti-TRFP/MED20 Picoband Antibody

Product Gene Name

anti-MED20 antibody

Product Synonym Names

Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

13,540 Da

OMIM

AF097725 mRNA

3D Structure

ModBase 3D Structure for Q9H944

Immunogen

E Coli-derived human TRFP/MED20 recombinant protein (Position: M1-R212).

Subcellular Localization

Nucleus.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-MED20 antibody

Epigenetics and Nuclear Signaling; Transcription; mediator complex

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MED20 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-MED20 antibody

WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml

Other Notes

Small volumes of anti-MED20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED20 purchase

MBS1750937 is an antibody targeting MED20. Also known as Anti-TRFP/MED20 Picoband Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MED20. It may not necessarily be applicable to this product.

NCBI GI #

768711647

NCBI GeneID

9477

NCBI Accession #

NP_001292384.1

UniProt Primary Accession #

Q9H944

UniProt Secondary Accession #

O95821; Q5T8J4; Q9Y429; B4DE08

UniProt Related Accession #

Q9H944

GenBank Nucleotide #

NM_001305455.1

NCBI Official Full Name

mediator of RNA polymerase II transcription subunit 20 isoform 2

NCBI Official Synonym Full Names

mediator complex subunit 20

NCBI Official Symbol

MED20

NCBI Official Synonym Symbols

SRB2; TRFP; PRO0213

UniProt Protein Name

Mediator of RNA polymerase II transcription subunit 20

UniProt Synonym Protein Names

Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP

Protein Family

Putative mediator of RNA polymerase II transcription

UniProt Gene Name

MED20

UniProt Synonym Gene Names

TRFP; hTRFP

NCBI Protein Information

mediator of RNA polymerase II transcription subunit 20

NCBI Summary for MED20

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

UniProt Comments for MED20

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Research Articles on MED20

1. Our analyses revealed no apparent differences in protein expression profiles of SRBs in central and peripheral regions of human donor tissues, indicating that carotenoid-binding proteins rather than transporters are likely to mediate selective accumulation of carotenoids into the macula.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MED20 antibody

Reference Product	PubMed Publications
MED18 antibody	>9 publications with MED20 and MED18
MED17 antibody	>2 publications with MED20 and MED17
CDK8 antibody	>2 publications with MED20 and CDK8

Pathways associated with anti-MED20 antibody

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Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
PPARA Activates Gene Expression Pathway antibodies	PPARA Activates Gene Expression Pathway Diagram
Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies	Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies	Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-MED20 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>3 publications with MED20 and Adenocarcinoma
Breast Neoplasms Antibodies	>3 publications with MED20 and Breast Neoplasms
Atrophy Antibodies	>1 publications with MED20 and Atrophy

Organs/Tissues associated with anti-MED20 antibody

Organ/Tissue Name	Pubmed Publications
Lymph Node Antibodies	>1 publications with MED20 and Lymph Node
Lymph Antibodies	>1 publications with MED20 and Lymph
Brain Antibodies	>1 publications with MED20 and Brain