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SCA1/Ly6A/E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750955

Description: Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Sca1/Ly6A/E, Polyclonal Antibody
Full Product Name
Anti-Sca1/Ly6A/E Picoband Antibody
Product Gene Name
anti-Sca1/Ly6A/E antibody
Product Synonym Gene Name
Ly6a
Product Synonym Names
Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; SCA-1; T-cell-activating protein; TAP; Ly6a; Ly6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
14377 MW
3D Structure
ModBase 3D Structure for P05533
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).
Subcellular Localization
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity
Widely expressed.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Sca1/Ly6A/E antibody
Western Blot (WB)
Application Notes for anti-Sca1/Ly6A/E antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-Sca1/Ly6A/E antibody

Figure 1. Western blot analysis of Sca1/Ly6A/E using anti- Sca1/Ly6A/E antibody (MBS1750955).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Sca1/Ly6A/E antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sca1/Ly6A/E at approximately 17KD. The expected band size for Sca1/Ly6A/E is at 14KD.
Rabbit Anti-SCA1/Ly6A/E Polyclonal Antibody – Cat# MBS1750955

Other Notes

Small volumes of anti-Sca1/Ly6A/E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Sca1/Ly6A/E purchase

MBS1750955 is an antibody targeting Sca1/Ly6A/E. Also known as Anti-Sca1/Ly6A/E Picoband Antibody. Reactive in Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Sca1/Ly6A/E. It may not necessarily be applicable to this product.

NCBI GI #
406855394
NCBI GeneID
110454
NCBI Accession #
NP_001258345.1
UniProt Primary Accession #
P05533
UniProt Secondary Accession #
Q8BPD0
UniProt Related Accession #
P05533
GenBank Nucleotide #
NM_001271416.1
NCBI Official Full Name
lymphocyte antigen 6A-2/6E-1
NCBI Official Synonym Full Names
lymphocyte antigen 6 complex, locus A
NCBI Official Symbol
Ly6a
NCBI Official Synonym Symbols
TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1
UniProt Protein Name
Lymphocyte antigen 6A-2/6E-1
UniProt Synonym Protein Names
Stem cell antigen 1; SCA-1; T-cell-activating protein; TAP
UniProt Gene Name
Ly6a
UniProt Synonym Gene Names
Ly6; Ly-6A.2/Ly-6E.1; SCA-1; TAP
NCBI Protein Information
lymphocyte antigen 6A-2/6E-1

UniProt Comments for Sca1/Ly6A/E

T-cell activation.

Research Articles on Sca1/Ly6A/E

1. Sca-1 is a novel cancer stem cells enrichment marker.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Sca1/Ly6A/E antibody

 Reference Product  PubMed Publications
 Cd34 antibody  >77 publications with Sca1/Ly6A/E and Cd34
 Ptprc antibody  >64 publications with Sca1/Ly6A/E and Ptprc
 Cd4 antibody  >21 publications with Sca1/Ly6A/E and Cd4
 Cd44 antibody  >20 publications with Sca1/Ly6A/E and Cd44
 Flt3 antibody  >12 publications with Sca1/Ly6A/E and Flt3
 Cd5 antibody  >3 publications with Sca1/Ly6A/E and Cd5

Diseases associated with anti-Sca1/Ly6A/E antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >63 publications with Sca1/Ly6A/E and Disease Models, Animal
 Inflammation Antibodies  >25 publications with Sca1/Ly6A/E and Inflammation
 Fibrosis Antibodies  >16 publications with Sca1/Ly6A/E and Fibrosis
 Arteriosclerosis Antibodies  >14 publications with Sca1/Ly6A/E and Arteriosclerosis
 Neoplasms, Experimental Antibodies  >14 publications with Sca1/Ly6A/E and Neoplasms, Experimental
 Necrosis Antibodies  >11 publications with Sca1/Ly6A/E and Necrosis
 Carcinoma Antibodies  >9 publications with Sca1/Ly6A/E and Carcinoma
 Hyperplasia Antibodies  >8 publications with Sca1/Ly6A/E and Hyperplasia
 Hypertrophy Antibodies  >6 publications with Sca1/Ly6A/E and Hypertrophy
 Lung Injury Antibodies  >6 publications with Sca1/Ly6A/E and Lung Injury

Organs/Tissues associated with anti-Sca1/Ly6A/E antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >256 publications with Sca1/Ly6A/E and Bone Marrow
 Blood Antibodies  >231 publications with Sca1/Ly6A/E and Blood
 Muscle Antibodies  >114 publications with Sca1/Ly6A/E and Muscle
 Spleen Antibodies  >71 publications with Sca1/Ly6A/E and Spleen
 Heart Antibodies  >68 publications with Sca1/Ly6A/E and Heart
 Liver Antibodies  >43 publications with Sca1/Ly6A/E and Liver
 Lung Antibodies  >36 publications with Sca1/Ly6A/E and Lung
 Embryonic Tissue Antibodies  >30 publications with Sca1/Ly6A/E and Embryonic Tissue
 Connective Tissue Antibodies  >23 publications with Sca1/Ly6A/E and Connective Tissue
 Skin Antibodies  >20 publications with Sca1/Ly6A/E and Skin
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