• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

C Reactive Protein/CRP (CRP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751226

Description: Rabbit IgG polyclonal antibody for C Reactive Protein detection. Tested with WB, IHC-P, Direct ELISA in Rat.Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
C Reactive Protein/CRP, Polyclonal Antibody
Full Product Name
Anti-C Reactive Protein/CRP Antibody
Product Gene Name
anti-CRP antibody
Product Synonym Names
C-reactive protein; Crp; Ptx1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Lyophilized; Each vial contains 4mg Trehalose , 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P48199
Immunogen
E Coli-derived rat C Reactive Protein recombinant protein (Position: H20-S230).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CRP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-CRP antibody
WB: 0.1-0.5 ug/ml
IHC-P: 0.5-1ug/ml; Antigen Retrieval: By Heat
Direct ELISA: 0.1-0.5 ug/ml
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-CRP antibody

Figure 1. Western blot analysis of C Reactive Protein using anti-C Reactive Protein antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C Reactive Protein antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C Reactive Protein at approximately 28KD. The expected band size for C Reactive Protein is at 25KD.
Rabbit Anti-CRP Polyclonal Antibody – Cat# MBS1751226

Immunohistochemistry (IHC) of anti-CRP antibody

Figure 3. IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody.
C Reactive Protein was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-C Reactive Protein antibody.
C Reactive Protein was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-C Reactive Protein antibody.
C Reactive Protein was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C Reactive Protein Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CRP Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751226

Product References and Citations for anti-CRP antibody

1. Kilpatrick, J. M.; Volanakis, J. E.: Molecular genetics, structure, and function of C-reactive protein. Immun. Res. 10: 43-53, 1991. 2. Kuta, A. E.; Baum, L. L.: C-reactive protein is produced by a small number of normal human peripheral blood lymphocytes. J. Exp. Med. 164: 321-326, 1986. 3. Oliveira, E. B.; Gotshlich, E. C.; Liu, T.: Primary structure of human C-reactive protein. J. Biol. Chem. 254: 489-502, 1979.

Other Notes

Small volumes of anti-CRP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRP purchase

MBS1751226 is an antibody targeting CRP. Also known as Anti-C Reactive Protein/CRP Antibody. Reactive in Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CRP. It may not necessarily be applicable to this product.

NCBI GI #
8393197
NCBI GeneID
25419
NCBI Accession #
NP_058792.1
UniProt Primary Accession #
P48199
UniProt Secondary Accession #
Q5BK94
UniProt Related Accession #
P48199
GenBank Nucleotide #
NM_017096.3
NCBI Official Full Name
C-reactive protein
NCBI Official Synonym Full Names
C-reactive protein
NCBI Official Symbol
Crp
NCBI Official Synonym Symbols
Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
UniProt Protein Name
C-reactive protein
Protein Family
C-reactive protein
UniProt Gene Name
Crp
UniProt Synonym Gene Names
Ptx1
NCBI Protein Information
C-reactive protein

NCBI Summary for CRP

glycoprotein of the acute phase response [RGD, Feb 2006]

UniProt Comments for CRP

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells ().
Show more

Research Articles on CRP

1. coordinated upregulation of genes encoding CRP, interleukin-6 (IL-6), HNF1alpha, and HNF4alpha in the livers and white adipose tissue (WAT) of chronic renal failure rats, as compared to the pair-fed and control animals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CRP antibody

 Reference Product  PubMed Publications
 C1qa antibody  >2 publications with CRP and C1qa

Diseases associated with anti-CRP antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >13276 publications with CRP and Inflammation
 Cardiovascular Diseases Antibodies  >9356 publications with CRP and Cardiovascular Diseases
 Infection Antibodies  >5163 publications with CRP and Infection
 Heart Diseases Antibodies  >4468 publications with CRP and Heart Diseases
 Metabolic Diseases Antibodies  >4328 publications with CRP and Metabolic Diseases
 Necrosis Antibodies  >3657 publications with CRP and Necrosis
 Myocardial Ischemia Antibodies  >3542 publications with CRP and Myocardial Ischemia
 Atherosclerosis Antibodies  >3124 publications with CRP and Atherosclerosis
 Obesity Antibodies  >2320 publications with CRP and Obesity
 Arthritis, Rheumatoid Antibodies  >2285 publications with CRP and Arthritis, Rheumatoid

Organs/Tissues associated with anti-CRP antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1640 publications with CRP and Liver
 Spleen Antibodies  >138 publications with CRP and Spleen
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000