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TNF Receptor I/TNFRSF1A (TNFRSF1A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751231

Background: Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), also known as TNFR1, is a protein that in humans is encoded by the TNFRSF1A gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily,… More details

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Product Overview

Online Datasheet in PDF
Product Name
TNF Receptor I/TNFRSF1A, Polyclonal Antibody
Full Product Name
Anti-TNF Receptor I/TNFRSF1A Antibody
Product Gene Name
anti-TNFRSF1A antibody
Product Synonym Names
Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD120a; Tumor necrosis factor receptor superfamily member 1A, membrane form; Tumor necrosis factor-binding protein 1; TBPI; TNFRSF1A; TNFAR; TNFR1; TNF receptor superfamily member 1A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for 6 months.
Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P19438
Immunogen
E. coli-derived human TNF Receptor I recombinant protein (Position: I22-T211).
Human TNF Receptor I shares 70.5% and 69.3% amino acid (aa) sequence identity with mouse and rat TNF Receptor I, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC-P, IHC-F and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TNFRSF1A antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC), ELISA Capture (EIA)
Application Notes for anti-TNFRSF1A antibody
WB: concentration: 0.1-0.5 ug/ml
IHC-P: concentration: 0.5-1 ug/ml
IHC-F: concentration: 0.5-1 ug/ml
ICC: concentration: 0.5-1 ug/ml
FC: 1-3ug/1x106 cells
ELISA (Cap): concentration: 1-5 ug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-TNFRSF1A antibody

Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human K562 whole cell lysate,
Lane 3: human Caco-2 whole cell lysate,
Lane 4: rat liver tissue lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Rabbit Anti-TNFRSF1A Polyclonal Antibody – Cat# MBS1751231

Immunohistochemistry (IHC) of anti-TNFRSF1A antibody

IHC analysis of TNF Receptor I using anti-TNF Receptor I antibody.
TNF Receptor I was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF Receptor I antibody.
TNF Receptor I was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF Receptor I antibody.
TNF Receptor I was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF Receptor I antibody.
Overlay histogram showing U937 cells stained with MBS1751231 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Receptor I Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-TNFRSF1A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751231

Product References and Citations for anti-TNFRSF1A antibody

1. Baker E, Chen LZ, Smith CA, Callen DF, Goodwin R, Sutherland GR (Nov 1991). "Chromosomal location of the human tumor necrosis factor receptor genes". Cytogenet Cell Genet 57 (2-3): 117-8. 2. Schall TJ, Lewis M, Koller KJ, Lee A, Rice GC, Wong GH, Gatanaga T, Granger GA, Lentz R, Raab H, et al. (Jun 1990). "Molecular cloning and expression of a receptor for human tumor necrosis factor". Cell 61 (2): 361-70.

Other Notes

Small volumes of anti-TNFRSF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFRSF1A purchase

MBS1751231 is an antibody targeting TNFRSF1A. Also known as Anti-TNF Receptor I/TNFRSF1A Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFRSF1A. It may not necessarily be applicable to this product.

NCBI GI #
4507575
NCBI GeneID
7132
NCBI Accession #
NP_001056.1
UniProt Primary Accession #
P19438
UniProt Secondary Accession #
Q9UCA4; A8K4X3; B2RDE4; B3KPQ1; B4DQB7; B4E309; B5M0B5; D3DUR1
UniProt Related Accession #
P19438
GenBank Nucleotide #
NM_001065.3
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 1A isoform 1
NCBI Official Synonym Full Names
TNF receptor superfamily member 1A
NCBI Official Symbol
TNFRSF1A
NCBI Official Synonym Symbols
FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1A
UniProt Synonym Protein Names
Tumor necrosis factor receptor 1; TNF-R1
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
TNFRSF1A
UniProt Synonym Gene Names
TNFAR; TNFR1; TNF-R1; TNF-RI; TNFR-I; TBPI
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1A

NCBI Summary for TNFRSF1A

This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]
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UniProt Comments for TNFRSF1A

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
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Research Articles on TNFRSF1A

1. by restraining TNFR1 at the cell surface via sialylation, ST6Gal-I acts as a functional switch to divert signaling toward survival. These collective findings point to a novel glycosylation-dependent mechanism that regulates the cellular response to TNF and may promote cancer cell survival within TNF-rich tumor microenvironments.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNFRSF1A antibody

 Reference Product  PubMed Publications
 TNF antibody  >351 publications with TNFRSF1A and TNF
 TRADD antibody  >12 publications with TNFRSF1A and TRADD
 TRAF2 antibody  >10 publications with TNFRSF1A and TRAF2
 IKBKB antibody  >9 publications with TNFRSF1A and IKBKB
 LTA antibody  >6 publications with TNFRSF1A and LTA
 TNFRSF25 antibody  >5 publications with TNFRSF1A and TNFRSF25
 RIPK1 antibody  >5 publications with TNFRSF1A and RIPK1
 MAPK1 antibody  >5 publications with TNFRSF1A and MAPK1
 RALBP1 antibody  >5 publications with TNFRSF1A and RALBP1
 ERAP1 antibody  >5 publications with TNFRSF1A and ERAP1

Pathways associated with anti-TNFRSF1A antibody

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 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram

Diseases associated with anti-TNFRSF1A antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >500 publications with TNFRSF1A and Necrosis
 Inflammation Antibodies  >163 publications with TNFRSF1A and Inflammation
 Fever Antibodies  >140 publications with TNFRSF1A and Fever
 Autoimmune Diseases Antibodies  >77 publications with TNFRSF1A and Autoimmune Diseases
 Pain Antibodies  >54 publications with TNFRSF1A and Pain
 Cardiovascular Diseases Antibodies  >53 publications with TNFRSF1A and Cardiovascular Diseases
 Neoplasms Antibodies  >37 publications with TNFRSF1A and Neoplasms
 Heart Diseases Antibodies  >32 publications with TNFRSF1A and Heart Diseases
 Drug Toxicity Antibodies  >30 publications with TNFRSF1A and Drug Toxicity
 Lung Diseases Antibodies  >28 publications with TNFRSF1A and Lung Diseases

Organs/Tissues associated with anti-TNFRSF1A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >184 publications with TNFRSF1A and Blood
 Brain Antibodies  >46 publications with TNFRSF1A and Brain
 Liver Antibodies  >45 publications with TNFRSF1A and Liver
 Skin Antibodies  >42 publications with TNFRSF1A and Skin
 Vascular Antibodies  >40 publications with TNFRSF1A and Vascular
 Muscle Antibodies  >38 publications with TNFRSF1A and Muscle
 Bone Antibodies  >38 publications with TNFRSF1A and Bone
 Lung Antibodies  >33 publications with TNFRSF1A and Lung
 Bone Marrow Antibodies  >26 publications with TNFRSF1A and Bone Marrow
 Heart Antibodies  >26 publications with TNFRSF1A and Heart
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