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CD47 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751237

Description: Rabbit IgG polyclonal antibody for CD47 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.Background: CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene.… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD47, Polyclonal Antibody
Full Product Name
Anti-CD47 Antibody
Product Gene Name
anti-CD47 antibody
Product Synonym Names
Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; MER6; CD47 molecule
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
31,743 Da
3D Structure
ModBase 3D Structure for Q08722
Immunogen
E Coli-derived human CD47 recombinant protein (Position: Q19-E323).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P), IHC(F) and ICC.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD47 antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-CD47 antibody
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml
ICC/IF: 2ug/ml
FC/FACS: 1-3mug/1x106
Direct ELISA: 0.1-0.5ug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.

Product References and Citations for anti-CD47 antibody

1. Bras, M., Yuste, V. J., Roue, G., Barbier, S., Sancho, P., Virely, C., Rubio, M., Baudet, S., Esquerda, J. E., Merle-Beral, H., Sarfati, M., Susin, S. A. Drp1 mediates caspase-independent type III cell death in normal and leukemic cells. Molec. Cell. Biol. 27: 7073-7088, 2007. 2. Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., Lindberg, F. P., Vignery, A. CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. J. Biol. Chem. 275: 37984-37992, 2000. 3. Oldenborg, P.-A., Zheleznyak, A., Fang, Y.-F., Lagenaur, C. F., Gresham, H. D., Lindberg, F. P. Role of CD47 as a marker of self on red blood cells. Science 288: 2051-2054, 2000.

Other Notes

Small volumes of anti-CD47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD47 purchase

MBS1751237 is an antibody targeting CD47. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.

NCBI GI #
4502673
NCBI GeneID
961
NCBI Accession #
NP_001768.1
UniProt Primary Accession #
Q08722
UniProt Secondary Accession #
Q53Y71; Q96A60; A8K198; D3DN59
UniProt Related Accession #
Q08722
GenBank Nucleotide #
NM_001777.3
NCBI Official Full Name
leukocyte surface antigen CD47 isoform 1
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Synonym Protein Names
Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47
Protein Family
Leukocyte surface antigen
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
NCBI Protein Information
leukocyte surface antigen CD47

NCBI Summary for CD47

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
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UniProt Comments for CD47

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
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Research Articles on CD47

1. overexpression of human CD200 in donor pigs could constitute a promising strategy for overcoming xenograft rejection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD47 antibody

 Reference Product  PubMed Publications
 THBS1 antibody  >176 publications with CD47 and THBS1
 SIRPA antibody  >83 publications with CD47 and SIRPA
 PTPN11 antibody  >4 publications with CD47 and PTPN11
 SIRPG antibody  >4 publications with CD47 and SIRPG
 ITGB3 antibody  >2 publications with CD47 and ITGB3
 ITGAV antibody  >2 publications with CD47 and ITGAV
 PTK2B antibody  >1 publications with CD47 and PTK2B

Pathways associated with anti-CD47 antibody

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-CD47 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >104 publications with CD47 and Inflammation
 Immune System Diseases Antibodies  >92 publications with CD47 and Immune System Diseases
 Disease Models, Animal Antibodies  >57 publications with CD47 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >48 publications with CD47 and Cardiovascular Diseases
 Anemia Antibodies  >47 publications with CD47 and Anemia
 Necrosis Antibodies  >44 publications with CD47 and Necrosis
 Nervous System Diseases Antibodies  >40 publications with CD47 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >28 publications with CD47 and Neoplasms, Experimental
 Lung Diseases Antibodies  >16 publications with CD47 and Lung Diseases
 Breast Neoplasms Antibodies  >13 publications with CD47 and Breast Neoplasms

Organs/Tissues associated with anti-CD47 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >483 publications with CD47 and Blood
 Vascular Antibodies  >112 publications with CD47 and Vascular
 Bone Antibodies  >85 publications with CD47 and Bone
 Bone Marrow Antibodies  >69 publications with CD47 and Bone Marrow
 Brain Antibodies  >64 publications with CD47 and Brain
 Muscle Antibodies  >57 publications with CD47 and Muscle
 Liver Antibodies  >37 publications with CD47 and Liver
 Lung Antibodies  >33 publications with CD47 and Lung
 Spleen Antibodies  >32 publications with CD47 and Spleen
 Lymph Node Antibodies  >31 publications with CD47 and Lymph Node
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