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CD5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751251

Description: Rabbit IgG polyclonal antibody for CD5 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human; Mouse; Rat.Background: CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD5, Polyclonal Antibody
Full Product Name
Anti-CD5 Antibody
Product Gene Name
anti-CD5 antibody
Product Synonym Names
T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1; CD5 molecule
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
54,578 Da
3D Structure
ModBase 3D Structure for P06127
Immunogen
E Coli-derived human CD5 recombinant protein (Position: R25-L495).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P, IHC-F and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-CD5 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
IHC-F: 0.5-1 mug/ml
ICC: 0.5-1 mug/ml
FC/FACS: 1-3ug/1x106 cells
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-CD5 antibody

Figure 1. Western blot analysis of CD5 using anti-CD5 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.
Rabbit Anti-CD5 Polyclonal Antibody – Cat# MBS1751251

Immunohistochemistry (IHC) of anti-CD5 antibody

Figure 3. IHC analysis of CD5 using anti-CD5 antibody.
CD5 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-CD5 antibody.
CD5 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-CD5 antibody.
CD5 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD5 antibody.
Overlay histogram showing EL-4 degree Cells stained with A00480-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD5 Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-CD5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751251

Product References and Citations for anti-CD5 antibody

1. Lundell, A.-C., Johansen, S., Adlerberth, I., Wold, A. E., Hesselmar, B., Rudin, A. High proportion of CD5+ B cells in infants predicts development of allergic disease. J. Immun. 193: 510-518, 2014. 2. Zhang, C., Xin, H., Zhang, W., Yazaki, P. J., Zhang, Z., Le, K., Li, W., Lee, H., Kwak, L., Forman, S., Jove, R., Yu, H. CD5 binds to interleukin-6 and induces a feed-forward loop with the transcription factor STAT3 in B cells to promote cancer. Immunity 44: 913-923, 2016.

Other Notes

Small volumes of anti-CD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD5 purchase

MBS1751251 is an antibody targeting CD5. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD5. It may not necessarily be applicable to this product.

NCBI GI #
1080078688
NCBI GeneID
921
NCBI Accession #
NP_001333385.1
UniProt Primary Accession #
P06127
UniProt Secondary Accession #
A0N0P4; A8K9I3
UniProt Related Accession #
P06127
GenBank Nucleotide #
NM_001346456.1
NCBI Official Full Name
T-cell surface glycoprotein CD5 isoform 2
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
UniProt Protein Name
T-cell surface glycoprotein CD5
UniProt Synonym Protein Names
Lymphocyte antigen T1/Leu-1; CD_antigen: CD5
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5

NCBI Summary for CD5

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
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UniProt Comments for CD5

May act as a receptor in regulating T-cell proliferation.

Research Articles on CD5

1. the present results support the notion that CD5 is not only a phenotypic marker but a relevant player in CLL cell biology, as demonstrated by the influence of germline-defined functional CD5 variants on CLL outcome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD5 antibody

 Reference Product  PubMed Publications
 CD19 antibody  >1241 publications with CD5 and CD19
 CD2 antibody  >799 publications with CD5 and CD2
 CD79A antibody  >211 publications with CD5 and CD79A
 CD6 antibody  >113 publications with CD5 and CD6
 BCL6 antibody  >91 publications with CD5 and BCL6
 ZAP70 antibody  >59 publications with CD5 and ZAP70
 LCK antibody  >32 publications with CD5 and LCK
 PTPRC antibody  >9 publications with CD5 and PTPRC
 CD40LG antibody  >1 publications with CD5 and CD40LG

Pathways associated with anti-CD5 antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD5 antibody

 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >102 publications with CD5 and Thrombocytopenia
 Depressive Disorder Antibodies  >2 publications with CD5 and Depressive Disorder
 Cognition Disorders Antibodies  >1 publications with CD5 and Cognition Disorders
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