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Cytochrome P450 17a1/Cyp17a1 (CYP17A1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751260

Description: Rabbit IgG polyclonal antibody for Cyp17a1 detection. Tested with WB, IHC-P, Direct ELISA in Mouse; Rat.Background: Cytochrome P450 17A1, also called steroid 17alpha-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cytochrome P450 17A1/Cyp17a1, Polyclonal Antibody
Full Product Name
Anti-Cytochrome P450 17A1/Cyp17a1 Antibody
Product Gene Name
anti-Cyp17a1 antibody
Product Synonym Names
Steroid 17-alpha-hydroxylase/17, 20 lyase; Cytochrome P450, family 17, subfamily a, polypeptide 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
57,638 Da
3D Structure
ModBase 3D Structure for P27786
Immunogen
E Coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Cyp17a1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-Cyp17a1 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-Cyp17a1 antibody

Figure 1. Western blot analysis of Cyp17a1 using anti-Cyp17a1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: mouse testis tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyp17a1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyp17a1 at approximately 57KD. The expected band size for Cyp17a1 is at 57KD.
Rabbit Anti-CYP17A1 Polyclonal Antibody – Cat# MBS1751260

Immunohistochemistry (IHC) of anti-Cyp17a1 antibody

Figure 3. IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody.
Cyp17a1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cyp17a1 antibody.
Cyp17a1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cyp17a1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CYP17A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751260

Product References and Citations for anti-Cyp17a1 antibody

1. DeVore NM, Scott EE (February 2012). "Structures of cytochrome P450 17A1 with prostate cancer drugs abiraterone and TOK-001". Nature. 482 (7383): 116-9. 2. Estrada DF, Laurence JS, Scott EE (February 2016). "Cytochrome P450 17A1 Interactions with the FMN Domain of Its Reductase as Characterized by NMR". The Journal of Biological Chemistry. 291 (8): 3990-4003. 3. Petrunak EM, DeVore NM, Porubsky PR, Scott EE (November 2014). "Structures of human steroidogenic cytochrome P450 17A1 with substrates".The Journal of Biological Chemistry. 289 (47): 32952-64.

Other Notes

Small volumes of anti-Cyp17a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cyp17a1 purchase

MBS1751260 is an antibody targeting Cyp17a1. Also known as Anti-Cytochrome P450 17A1/Cyp17a1 Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cyp17a1. It may not necessarily be applicable to this product.

NCBI GI #
160948601
NCBI GeneID
13074
NCBI Accession #
NP_031835.3
UniProt Primary Accession #
P27786
UniProt Related Accession #
P27786
GenBank Nucleotide #
NM_007809.3
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450, family 17, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp17a1
NCBI Official Synonym Symbols
Cyp17; p450c17
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17
Protein Family
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Gene Name
Cyp17a1
UniProt Synonym Gene Names
Cyp17; Cytochrome P450c17
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase

UniProt Comments for Cyp17a1

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Research Articles on Cyp17a1

1. through mouse-to-human search and validation, we found that CYP17A1 is overexpressed in hepatocellular carcinomas and it has great potentiality as a noninvasive marker for hepatocellular carcinoma detection

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cyp17a1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 Cytochrome P450 Pathway antibodies  Cytochrome P450 Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Endogenous Sterols Pathway antibodies  Endogenous Sterols Pathway Diagram
 Glucocorticoid & Mineralcorticoid Metabolism Pathway antibodies  Glucocorticoid & Mineralcorticoid Metabolism Pathway Diagram

Diseases associated with anti-Cyp17a1 antibody

 Disease Name  Pubmed Publications
 Hyperplasia Antibodies  >317 publications with Cyp17a1 and Hyperplasia
 Adrenal Hyperplasia, Congenital Antibodies  >268 publications with Cyp17a1 and Adrenal Hyperplasia, Congenital
 Genital Diseases, Male Antibodies  >261 publications with Cyp17a1 and Genital Diseases, Male
 Prostatic Neoplasms Antibodies  >207 publications with Cyp17a1 and Prostatic Neoplasms
 Hypertension Antibodies  >169 publications with Cyp17a1 and Hypertension
 Breast Neoplasms Antibodies  >110 publications with Cyp17a1 and Breast Neoplasms
 Ovarian Cysts Antibodies  >103 publications with Cyp17a1 and Ovarian Cysts
 Cardiovascular Diseases Antibodies  >94 publications with Cyp17a1 and Cardiovascular Diseases
 Amenorrhea Antibodies  >60 publications with Cyp17a1 and Amenorrhea
 Hypogonadism Antibodies  >47 publications with Cyp17a1 and Hypogonadism

Organs/Tissues associated with anti-Cyp17a1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >583 publications with Cyp17a1 and Testis
 Liver Antibodies  >112 publications with Cyp17a1 and Liver
 Embryonic Tissue Antibodies  >60 publications with Cyp17a1 and Embryonic Tissue
 Muscle Antibodies  >28 publications with Cyp17a1 and Muscle
 Skin Antibodies  >20 publications with Cyp17a1 and Skin
 Uncharacterized Tissue Antibodies  >1 publications with Cyp17a1 and Uncharacterized Tissue
 Extraembryonic Tissue Antibodies  >1 publications with Cyp17a1 and Extraembryonic Tissue
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