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SPARC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751281

Description: Rabbit IgG polyclonal antibody for SPARC detection. Tested with WB, Direct ELISA in Human; Mouse; Rat.Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SPARC, Polyclonal Antibody
Full Product Name
Anti-SPARC Antibody
Product Gene Name
anti-SPARC antibody
Product Synonym Names
SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine; Sparc; Secreted acidic cysteine rich glycoprotein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
34,450 Da
3D Structure
ModBase 3D Structure for P07214
Immunogen
E Coli-derived mouse SPARC recombinant protein (Position: H123-L300).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SPARC antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes for anti-SPARC antibody
WB: 0.1-0.5 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Western Blot (WB) of anti-SPARC antibody

Figure 1. Western blot analysis of SPARC using anti-SPARC antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SPARC at approximately 37KD. The expected band size for SPARC is at 35KD.
Rabbit Anti-SPARC Polyclonal Antibody – Cat# MBS1751281

Product References and Citations for anti-SPARC antibody

1. Gilmour, D. T., Lyon, G. J., Carlton, M. B. L., Sanes, J. R., Cunningham, J. M., Anderson, J. R., Hogan, B. L. M., Evans, M. J., Colledge, W. H.Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens.EMBO J. 17: 1860-1870, 1998. 2. Goldblum, S. E., Ding, X., Funk, S. E., Sage, E. H.SPARC (secreted protein acidic and rich in cysteine) regulates endothelial cell shape and barrier function.Proc. Nat. Acad. Sci. 91: 3448-3452, 1994. 3. Minn, A. J., Gupta, G. P., Siegel, P. M., Bos, P. D., Shu, W., Giri, D. D., Viale, A., Olshen, A. B., Gerald, W. L., Massague, J.Genes that mediate breast cancer metastasis to lung.Nature 436: 518-524, 2005.

Other Notes

Small volumes of anti-SPARC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPARC purchase

MBS1751281 is an antibody targeting SPARC. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPARC. It may not necessarily be applicable to this product.

NCBI GI #
6678077
NCBI GeneID
20692
NCBI Accession #
NP_033268.1
UniProt Primary Accession #
P07214
UniProt Related Accession #
P07214
GenBank Nucleotide #
NM_009242.5
NCBI Official Full Name
SPARC
NCBI Official Synonym Full Names
secreted acidic cysteine rich glycoprotein
NCBI Official Symbol
Sparc
NCBI Official Synonym Symbols
ON; BM-40
UniProt Protein Name
SPARC
UniProt Synonym Protein Names
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Protein Family
SPARC
UniProt Gene Name
Sparc
UniProt Synonym Gene Names
BM-40; ON
NCBI Protein Information
SPARC

UniProt Comments for SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
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Research Articles on SPARC

1. It is a matricellular glycol that regulates cell proliferation, tissue repair, and tumorigenesis and a potential therapeutic target for conditions involving intestinal inflammation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPARC antibody

 Reference Product  PubMed Publications
 Tgfb1 antibody  >77 publications with SPARC and Tgfb1
 Col1a1 antibody  >44 publications with SPARC and Col1a1
 Col1a2 antibody  >43 publications with SPARC and Col1a2
 Col3a1 antibody  >25 publications with SPARC and Col3a1
 Tgfb2 antibody  >19 publications with SPARC and Tgfb2
 Fn1 antibody  >17 publications with SPARC and Fn1
 Vegfa antibody  >10 publications with SPARC and Vegfa
 Timp1 antibody  >10 publications with SPARC and Timp1
 Serpine1 antibody  >5 publications with SPARC and Serpine1
 Plg antibody  >2 publications with SPARC and Plg

Pathways associated with anti-SPARC antibody

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Vesicle-mediated Transport Pathway antibodies  Vesicle-mediated Transport Pathway Diagram

Diseases associated with anti-SPARC antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >137 publications with SPARC and Adenocarcinoma
 Disease Models, Animal Antibodies  >124 publications with SPARC and Disease Models, Animal
 Inflammation Antibodies  >120 publications with SPARC and Inflammation
 Fibrosis Antibodies  >111 publications with SPARC and Fibrosis
 Breast Neoplasms Antibodies  >94 publications with SPARC and Breast Neoplasms
 Necrosis Antibodies  >71 publications with SPARC and Necrosis
 Lung Neoplasms Antibodies  >59 publications with SPARC and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >56 publications with SPARC and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >52 publications with SPARC and Cell Transformation, Neoplastic
 Colorectal Neoplasms Antibodies  >48 publications with SPARC and Colorectal Neoplasms

Organs/Tissues associated with anti-SPARC antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1186 publications with SPARC and Bone
 Connective Tissue Antibodies  >484 publications with SPARC and Connective Tissue
 Blood Antibodies  >369 publications with SPARC and Blood
 Bone Marrow Antibodies  >229 publications with SPARC and Bone Marrow
 Embryonic Tissue Antibodies  >184 publications with SPARC and Embryonic Tissue
 Brain Antibodies  >168 publications with SPARC and Brain
 Skin Antibodies  >134 publications with SPARC and Skin
 Heart Antibodies  >134 publications with SPARC and Heart
 Eye Antibodies  >130 publications with SPARC and Eye
 Lung Antibodies  >123 publications with SPARC and Lung
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