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Mannose 6 Phosphate Receptor/Igf2r (IGF2R) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751289

Description: Rabbit IgG polyclonal antibody for Igf2r detection. Tested with WB, IHC-P in Mouse; Rat.Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to… More details

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Product Overview

Online Datasheet in PDF
Product Name
Mannose 6 Phosphate Receptor/Igf2r, Polyclonal Antibody
Full Product Name
Anti-Mannose 6 Phosphate Receptor/Igf2r Antibody
Product Gene Name
anti-Igf2r antibody
Product Synonym Names
Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; M6PR; 300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD222; Igf2r
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
273,815 Da
3D Structure
ModBase 3D Structure for Q07113
Immunogen
A synthetic peptide corresponding to a sequence of mouse Igf2r (EKARKGKFRP GQRKPTAPAKLVSFHDDSDEDLLH).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Igf2r antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-Igf2r antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Immunohistochemistry (IHC) of anti-Igf2r antibody

Figure 2. IHC analysis of Igf2r using anti-Igf2r antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: mouse NIH3T3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat stomach tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Igf2r antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Igf2r at approximately 300KD. The expected band size for Igf2r is at 274KD.
Rabbit Anti-IGF2R Polyclonal Antibody – Cat# MBS1751289

Western Blot (WB) of anti-Igf2r antibody

Figure 1. Western blot analysis of Igf2r using anti-Igf2r antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: mouse NIH3T3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat stomach tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Igf2r antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Igf2r at approximately 300KD. The expected band size for Igf2r is at 274KD.
Rabbit Anti-IGF2R Polyclonal Antibody, Western Blot (WB) – Cat# MBS1751289

Product References and Citations for anti-Igf2r antibody

1. Killian, J. K., Oka, Y., Jang, H.-S., Fu, X., Waterland, R. A., Sohda, T., Sakaguchi, S., Jirtle, R. L. Mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) variants in American and Japanese populations. Hum. Mutat. 18: 25-31, 2001. 2. Latos, P. A., Pauler, F. M., Koerner, M. V., Senergin, H. B., Hudson, Q. J., Stocsits, R. R., Allhoff, W., Stricker, S. H., Klement, R. M., Warczok, K. E., Aumayr, K., Pasierbek, P., Barlow, D. P. Airn transcriptional overlap, but not its IncRNA products, induces imprinted Igf2r silencing. Science 338: 1469-1472, 2012.

Other Notes

Small volumes of anti-Igf2r antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Igf2r purchase

MBS1751289 is an antibody targeting Igf2r. Also known as Anti-Mannose 6 Phosphate Receptor/Igf2r Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Igf2r. It may not necessarily be applicable to this product.

NCBI GI #
133778978
NCBI GeneID
16004
NCBI Accession #
NP_034645.2
UniProt Primary Accession #
Q07113
UniProt Secondary Accession #
Q61822; Q6LED1
UniProt Related Accession #
Q07113
GenBank Nucleotide #
NM_010515.2
NCBI Official Full Name
cation-independent mannose-6-phosphate receptor
NCBI Official Synonym Full Names
insulin-like growth factor 2 receptor
NCBI Official Symbol
Igf2r
NCBI Official Synonym Symbols
CD222; CI-MPR; Mpr300; AI661837; M6P/IGF2R
UniProt Protein Name
Cation-independent mannose-6-phosphate receptor
UniProt Synonym Protein Names
300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD_antigen: CD222
Protein Family
Cation-independent mannose-6-phosphate receptor
UniProt Gene Name
Igf2r
UniProt Synonym Gene Names
CI Man-6-P receptor; CI-MPR; M6PR; MPR 300; IGF-II receptor; M6P/IGF2R
NCBI Protein Information
cation-independent mannose-6-phosphate receptor

UniProt Comments for Igf2r

Acts as a positive regulator of T-cell coactivation, by binding DPP4 (). Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.
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Research Articles on Igf2r

1. After 6 weeks of environmental tobacco smoke exposure, the Sirt-1 protein level was decreased and cardiac autophagy was increased in C57BL mice. Furthermore, the IGF2R cardiac hypertrophy signaling pathway was also triggered, although cardiac apoptosis and hypertrophy were not induced.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Igf2r antibody

 Products by Pathway  Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 PI3K/Akt/mTOR Signaling Pathway antibodies  PI3K/Akt/mTOR Signaling Pathway Diagram
 Trans-Golgi Network Vesicle Budding Pathway antibodies  Trans-Golgi Network Vesicle Budding Pathway Diagram

Diseases associated with anti-Igf2r antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >97 publications with Igf2r and Neoplasms
 Carcinoma Antibodies  >47 publications with Igf2r and Carcinoma
 Adenocarcinoma Antibodies  >31 publications with Igf2r and Adenocarcinoma
 Liver Neoplasms Antibodies  >27 publications with Igf2r and Liver Neoplasms
 Liver Diseases Antibodies  >25 publications with Igf2r and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >21 publications with Igf2r and Carcinoma, Hepatocellular
 Skin Diseases Antibodies  >20 publications with Igf2r and Skin Diseases
 Breast Neoplasms Antibodies  >20 publications with Igf2r and Breast Neoplasms
 Death Antibodies  >19 publications with Igf2r and Death
 Cardiovascular Diseases Antibodies  >11 publications with Igf2r and Cardiovascular Diseases

Organs/Tissues associated with anti-Igf2r antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >56 publications with Igf2r and Liver
 Embryonic Tissue Antibodies  >54 publications with Igf2r and Embryonic Tissue
 Blood Antibodies  >32 publications with Igf2r and Blood
 Muscle Antibodies  >32 publications with Igf2r and Muscle
 Brain Antibodies  >26 publications with Igf2r and Brain
 Heart Antibodies  >25 publications with Igf2r and Heart
 Lung Antibodies  >20 publications with Igf2r and Lung
 Ovary Antibodies  >15 publications with Igf2r and Ovary
 Bone Antibodies  >12 publications with Igf2r and Bone
 Skin Antibodies  >10 publications with Igf2r and Skin
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